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Protein Overview: LEU4

General Information:
Name(s) found: LEU4 / YNL104C [SGD]
Description(s) found:
Found 26 descriptions.
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  • Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 619 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed articleLink to PubMed articleLink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed articleLink to PubMed articleLink to PubMed articleLink to PubMed article]
    Biological Process: leucine biosynthetic process [IMP]
    Molecular Function: 2-isopropylmalate synthase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKESIIALA EHAASRASRV IPPVKLAYKN MLKDPSSKYK PFNAPKLSNR KWPDNRITRA  60
   61 PRWLSTDLRD GNQSLPDPMS VEQKKEYFHK LVNIGFKEIE VSFPSASQTD FDFTRYAVEN 120
  121 APDDVSIQCL VQSREHLIKR TVEALTGAKK ATIHTYLATS DMFREIVFNM SREEAISKAV 180
  181 EATKLVRKLT KDDPSQQATR WSYEFSPECF SDTPGEFAVE ICEAVKKAWE PTEENPIIFN 240
  241 LPATVEVASP NVYADQIEYF ATHITEREKV CISTHCHNDR GCGVAATELG MLAGADRVEG 300
  301 CLFGNGERTG NVDLVTVAMN MYTQGVSPNL DFSDLTSVLD VVERCNKIPV SQRAPYGGDL 360
  361 VVCAFSGSHQ DAIKKGFNLQ NKKRAQGETQ WRIPYLPLDP KDIGRDYEAV IRVNSQSGKG 420
  421 GAAWVILRSL GLDLPRNMQI EFSSAVQDHA DSLGRELKSD EISKLFKEAY NYNDEQYQAI 480
  481 SLVNYNVEKF GTERRVFTGQ VKVGDQIVDI EGTGNGPISS LVDALSNLLN VRFAVANYTE 540
  541 HSLGSGSSTQ AASYIHLSYR RNADNEKAYK WGVGVSEDVG DSSVRAIFAT INNIIHSGDV 600
  601 SIPSLAEVEG KNAAASGSA

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) LEU4 LEU9
    View Details Krogan NJ, et al. (2006) LEU4 LEU9

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data LEU4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..476] ORFEUS 7.73 Methylmalonyl-CoA mutase alpha subunit, domain 1; Methylmalonyl-CoA mutase alpha subunit, C-terminal domain
    2 View Details [477..619] MSA 2.118979 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • 2-isopropylmalate synthase activity
    		•  3.07962096729216 bayes_pls_golite062009
  • transferase activity, transferring aldehyde or ketonic groups
    		•  2.96685031644778 bayes_pls_golite062009
  • catalytic activity
    		•  2.67031204377352 bayes_pls_golite062009
  • lyase activity
    		•  0.740424046054758 bayes_pls_golite062009
  • porphobilinogen synthase activity
    		•  0.658378297259497 bayes_pls_golite062009
  • transaldolase activity
    		•  0.184100272651834 bayes_pls_golite062009
  • binding
    		•  0.155633578635864 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.87

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle