Protein Overview: SPO1

General Information:
Name(s) found:	SPO1 / YNL012W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|9755342\|ref\|NP_014386.2\| Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation; Spo1p [Saccharomyces cerevisiae] [NCBI NR] gi\|114152880\|sp\|P53541.2\|SPO1_YEAST RecName: Full=Putative meiotic phospholipase SPO1; AltName: Full=Sporulation-specific protein 1; Flags: Precursor [NCBI NR] Putative meiotic phospholipase SPO1 OS=Saccharomyces cerevisiae GN=SPO1 PE=1 SV=2 [Swiss-Prot] SPO1 Transcriptional regulator involved in sporulation [mips] Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B [SGD] SPO1, Chr XIV from 609682-609786,609871-611661 [yeast_orfs2] Chr XIV from 609682-609786,609871-611661 [yeastorf3.fasta] SPO1 SGDID:S0004957, Chr XIV from 609684-609788,609873-611663, Verified ORF [yeastflysequest.fasta] SPO1 SGDID:S0004957, Chr XIV from 609685-609789,609874-611664, Verified ORF [10112005cervSGD.fasta] Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] SPO1 SGDID:S000004957, Chr XIV from 609687-609791,609876-611666, Verified ORF, "Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SPO1 SGDID:S000004957, Chr XIV from 609687-609791,609876-611666, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Putative meiotic phospholipase SPO1 (Sporulation-specific protein 1) [nr-271106-contam.fasta] Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation; Spo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SPO1 SGDID:S000004957, Chr XIV from 609688-609792,609877-611667, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] SPO1 SGDID:S000004957, Chr XIV from 609687-609791,609876-611666, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B" [scerevisihumanrev.fasta] [chemostatdb.fasta] SPO1 SGDID:S000004957, Chr XIV from 609688-609792,609877-611667, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B" [SGDyeast.20101221.fasta] Putative meiotic phospholipase SPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPO1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPO1 SGDID:S000004957, Chr XIV from 609686-609790,609875-611665, Genome Release 64-1-1, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B" [yeast-030211-contam.fasta] SPO1 SGDID:S000004957, Chr XIV from 609686-609790,609875-611665, Genome Release 64-2-1, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B" [20141208_orf_trans.fasta] YNL012W SGDID:S000004957, Chr XIV from 609686-609790,609875-611665, Genome Release 64-1-1, Verified ORF, "Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	631 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] endoplasmic reticulum [IDA]
Biological Process:	meiosis [IMP]
Molecular Function:	phospholipase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQKLLFVFSV LLTVVLATAP FQVQCPSSPL IREAKHELCP EETLYLKKKK IKTKNKLIQF  60
   61 LKSLTEAKFS SKFYKRVLKD PPKIGIAISG GGYRSMLVGT GFISQMNDYG LFEYSDYIAG 120
  121 LSGGSWILMD LVVQNFEVKS LLQEWDLEED LLLGIPEFDI SEEEIVTNAK KEYNDNDLKM 180
  181 KKRQGGSLIT SSSNFYEQIE EIMNSIEEIP EDYMITKRNL NPLARLKKIF FPNNTFTGTD 240
  241 AKIETFKKVL DFYKSLHLKI KPKKMEGFQI SFTDYWGKAI VQRLKKNFDD DPNHSFSFSK 300
  301 LVNSSKKFKE CSVPIPIFVA NCKNGLLSNV IFEFTPFEFG SWENILRLFV KLPYLGSKIV 360
  361 SGKAEKCINN FDDLGFITAT SSSIFNNVLI FIWNLASQSS REAMKALNMV MGIFGLGKEE 420
  421 IFSISKDSSR LETDYAVYQP NPFYLYPEKD NVLTNKNHLY LVDGGEDGEN IPLRTLVIPE 480
  481 RELDVIFVLD SSSDIDNYPN GSKLKRIFEK LDEENVHYQF PNNVKTFTHP IVIGCNATKR 540
  541 TGHDSFLPII IYHANANHGN ASNTSTFKIT YNQSEVSSML PTGRGVFSND YDLYYKNCLG 600
  601 CILTKRTMDR LPRKKKFSPF CLQCFKDYCY S

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPO1	DDR2	2	Unpublished Fields Lab Data
View Screen	SPO1	SPO23	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..631]	PSI-BLAST	93.522879	Domain from cytosolic phospholipase A2; Cytosolic phospholipase A2 catalytic domain
2	View Details	[563..631]	FFAS03	1.74	Domain from cytosolic phospholipase A2; Cytosolic phospholipase A2 catalytic domain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2