Protein Overview: NGL3

General Information:
Name(s) found:	NGL3 / YML118W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323517\|ref\|NP_013588.1\| Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p; Ngl3p [Saccharomyces cerevisiae] [NCBI NR] gi\|2497064\|sp\|Q03210.1\|NGL3_YEAST RecName: Full=Probable RNA exonuclease NGL3 [NCBI NR] pir\|\|S54066 hypothetical protein YML118w - yeast (Saccharomyces cerevisiae) [NCBI NR] (Q03210) Hypothetical 57.7 kDa protein in NDI1-ATR1 intergenic region [Swiss-Prot] Probable RNA exonuclease NGL3 OS=Saccharomyces cerevisiae GN=NGL3 PE=1 SV=1 [Swiss-Prot] NGL3 DNase/RNase (putative) [mips] Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p [SGD] NGL3, Chr XIII from 32334-33851 [yeast_orfs2] Chr XIII from 32334-33851 [yeastorf3.fasta] NGL3 SGDID:S0004587, Chr XIII from 32334-33851, Verified ORF [yeastflysequest.fasta] DNase/RNase (putative); CCR4 C-terminal homolog; displays homology to drosophila Angel gene; homolog to ngl1 and ngl2 [Saccharomyces cerevisiae]gi\|2497064\|sp\|Q03210\|YML8_YEAST Hypothetical 57.7 kDa protein in NDI1-ATR1 intergenic regiongi\|2131829\|pir\|\|S [nrdb_11152004_Chlamydomonas] NGL3 SGDID:S000004587, Chr XIII from 32334-33851, Verified ORF, "Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Probable RNA exonuclease NGL3 [nr-271106-contam.fasta] Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p; Ngl3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Probable RNA exonuclease NGL3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NGL3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NGL3 SGDID:S000004587, Chr XIII from 32334-33851, Genome Release 64-1-1, Verified ORF, "Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p" [yeast-030211-contam.fasta] NGL3 SGDID:S000004587, Chr XIII from 32334-33851, Genome Release 64-2-1, Verified ORF, "3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YML118W SGDID:S000004587, Chr XIII from 32334-33851, Genome Release 64-1-1, Verified ORF, "Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	505 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	biological_process [ND]
Molecular Function:	endonuclease activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSQVEGKIS PSQKESSSTS GLVSPSEDGP AHQKIHRDQL SVDQIKKIRE ERAQKRQVRR  60
   61 NSLISQGKDP DFPTPDLQFI ERPFLPINHD NSKGLTPATI QVTQDSLDVK IMTYNTLAQT 120
  121 LIRRDFFPES GPALKWHKRS KVLVHELKKY RPDVVSLQEV DYNELNFWQE NFHKLGFDVI 180
  181 FKRHEGKTHG LLVAWNNKKF QLDNDWMLDY DNILAGNVIS ARTRTKNIAL IISLYFKGIT 240
  241 DSSSRGIIVA NTHLFWHPFG VFERLRQSYL VLQKIQEIKA CSKYNGWHSL LMGDFNTEPE 300
  301 EPPYLAITKR PLILKGPIRA MVECSLAYRY SKKRNGEESD QDDEECDEKS RGEGHSDQPQ 360
  361 NPKPESFTAT KEEKALVNQL VALHNSLHVK GVSLYGIGYG KVHPENANGS HGEPGLSNWA 420
  421 NTWCGLLDYI FYIEGDHNQD TRQKEPLNAF EGNNNVKIIG YLRMPCAQEM PKHSQPFEGE 480
  481 YASDHISLMC QIRLFFGGEK VHSLK

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PRR1	NGL3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..407]	PSI-BLAST	50.0	DNA repair endonuclease Hap1
2	View Details	[408..505]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
hydrolase activity, acting on ester bonds	1.79712722403488	bayes_pls_golite062009
catalytic activity	1.76511376991978	bayes_pls_golite062009
DNA binding	1.37658575624902	bayes_pls_golite062009
nuclease activity	1.32173587797854	bayes_pls_golite062009
nucleic acid binding	1.27522193028878	bayes_pls_golite062009
transcription regulator activity	1.27444635886918	bayes_pls_golite062009
hydrolase activity	1.26623219005539	bayes_pls_golite062009
binding	1.15309487870289	bayes_pls_golite062009
exonuclease activity	1.00241970456165	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.921825229691632	bayes_pls_golite062009
deoxyribonuclease activity	0.918937034405287	bayes_pls_golite062009
phosphoric ester hydrolase activity	0.483115797781113	bayes_pls_golite062009
phosphatase activity	0.384661571898828	bayes_pls_golite062009
protein binding	0.245431403492424	bayes_pls_golite062009
exodeoxyribonuclease activity	0.234646673057692	bayes_pls_golite062009
inositol or phosphatidylinositol phosphatase activity	0.228910502815798	bayes_pls_golite062009
exodeoxyribonuclease activity, producing 5'-phosphomonoesters	0.218294880470342	bayes_pls_golite062009
transcription activator activity	0.110687210838864	bayes_pls_golite062009
transcription cofactor activity	0.0749803326585898	bayes_pls_golite062009
transcription factor binding	0.0509794286384297	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2