YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: PIF1

General Information:
Name(s) found: PIF1 / YML061C [SGD]
Description(s) found:
SHOW ONLY BEST
  • gi|6323579|ref|NP_013650.1| DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA; Pif1p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|193806354|sp|P07271.2|PIF1_YEAST RecName: Full=DNA repair and recombination protein PIF1; AltName: Full=ATP-dependent helicase PIF1; AltName: Full=Telomere stability protein 1; Flags: Precursor [NCBI NR]
  • DNA repair and recombination protein PIF1 OS=Saccharomyces cerevisiae GN=PIF1 PE=1 SV=2 [Swiss-Prot]
  • PIF1 DNA helicase involved in mitochondrial DNA repair and telomere length control [mips]
  • DNA helicase; exists in two forms; the nuclear form is involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; the mitochondrial form is involved in repair and recombination of mitochondrial DNA [SGD]
  • PIF1, Chr XIII from 148953-151532, reverse complement [yeast_orfs2]
  • Chr XIII from 148953-151532, reverse complement [yeastorf3.fasta]
  • Chr XIII from 151532-148953, reverse complement [yeastorf5.fasta]
  • PIF1 SGDID:S0004526, Chr XIII from 151532-148953, reverse complement, Verified ORF [yeastflysequest.fasta]
  • Pif1p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, reverse complement, Verified ORF, "DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA; Pif1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, reverse complement, Verified ORF, "DNA helicase; exists in two forms; the nuclear form is involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; the mitochondrial for [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, reverse complement, Verified ORF, "DNA helicase involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; also plays a role in repair and recombination of mitochondrial DNA" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, reverse complement, Verified ORF, "DNA helicase; exists in two forms; the nuclear form is involved in telomere formation and elongation; acts as a catalytic inhibitor of telomerase; the mitochondrial form is involved in repair and recombination of mitochondrial DNA" [SGDyeast.20101221.fasta]
  • DNA repair and recombination protein PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIF1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, Genome Release 64-1-1, reverse complement, Verified ORF, "DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis" [yeast-030211-contam.fasta]
  • PIF1 SGDID:S000004526, Chr XIII from 151532-148953, Genome Release 64-2-1, reverse complement, Verified ORF, "DNA helicase, potent G-quadruplex DNA binder/unwinder; promotes DNA synthesis during break-induced replication (BIR); important for crossover recombination; translated from different start sites for two forms localized to either mitochondria or nucleus; nuclear form is catalytic inhibitor of telomerase; mitochondrial form involved in mitochondrial DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells" [20141208_orf_trans.fasta]
  • ATP-dependent DNA helicase PIF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIF1 PE=1 SV=2 [ACHE_BCHE_APH_CES_allEnz_HUMAN.fasta]
  • YML061C SGDID:S000004526, Chr XIII from 151532-148953, Genome Release 64-1-1, reverse complement, Verified ORF, "DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 859 amino acids

    Gene Ontology:
    Cellular Component: nucleus [ICLink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed articleLink to PubMed article]
    Biological Process: DNA recombination [IMPLink to PubMed article]
    telomere maintenance [IMPLink to PubMed articleLink to PubMed article]
    mitochondrial genome maintenance [IMPLink to PubMed article]
    Molecular Function: DNA helicase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF IQAQLKHPSI  60
   61 LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED PVDKKPMRDK NVMNFINKDS 120
  121 PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK KSRSTGFKNP LRPALKKESS FDELQNNSIS 180
  181 QERSLEMINE NEKKKMQFGE KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE 240
  241 QESIIKLAEN GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI 300
  301 TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL LDKLDFIARK 360
  361 IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK AWKEGVKMTI MLQKVFRQRG 420
  421 DVKFIDMLNR MRLGNIDDET EREFKKLSRP LPDDEIIPAE LYSTRMEVER ANNSRLSKLP 480
  481 GQVHIFNAID GGALEDEELK ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI 540
  541 EFMDPETYFC YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE 600
  601 GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS AGKRRLPLVR 660
  661 FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW SLSIHKSQGQ TLPKVKVDLR 720
  721 RVFEKGQAYV ALSRAVSREG LQVLNFDRTR IKAHQKVIDF YLTLSSAESA YKQLEADEQV 780
  781 KKRKLDYAPG PKYKAKSKSK SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV 840
  841 HSLVSDEPRG QDTEDHILE

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run NOP9 No Comments Hazbun TR, et al. (2003)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PIF1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..133] deduced N/A No confident structure predictions are available.
    2 View Details [134..236] PSI-BLAST 3.221849 Signal recognition particle receptor, FtsY; GTPase domain of the signal recognition particle receptor FtsY
    3 View Details [237..515] PSI-BLAST 37.69897 DEXX box DNA helicase
    4 View Details [516..634] PSI-BLAST 37.69897 DEXX box DNA helicase
    5 View Details [635..778] MSA 26.132995 View MSA. No confident structure predictions are available.
    6 View Details [779..859] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • binding
    		•  2.5815673740955 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.18361843860268 bayes_pls_golite062009
  • DNA binding
    		•  1.93283541470819 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.86711849769952 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  1.22434861137232 bayes_pls_golite062009
  • DNA-dependent ATPase activity
    		•  1.00145424382707 bayes_pls_golite062009
  • catalytic activity
    		•  0.942640875796745 bayes_pls_golite062009
  • helicase activity
    		•  0.930824665371921 bayes_pls_golite062009
  • transcription factor activity
    		•  0.737538356360809 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.723025725174193 bayes_pls_golite062009
  • DNA helicase activity
    		•  0.525155854231881 bayes_pls_golite062009
  • ATP-dependent helicase activity
    		•  0.364091846246035 bayes_pls_golite062009
  • purine NTP-dependent helicase activity
    		•  0.364091846246035 bayes_pls_golite062009
  • protein binding
    		•  0.201869978778282 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.164280007805333 bayes_pls_golite062009
  • ATP-dependent DNA helicase activity
    		•  0.0142902906813618 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle