Protein Overview: ASK1

General Information:
Name(s) found:	ASK1 / YKL052C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322799\|ref\|NP_012872.1\| Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kina [NCBI NR] gi\|549679\|sp\|P35734\|ASK1_YEAST DASH complex subunit ASK1 (Outer kinetochore protein ASK1) (Associated with spindles and kinetochores protein 1) [NCBI NR] pir\|\|S37874 hypothetical protein YKL052c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|51472787\|tpg\|DAA01815.1\| TPA_exp: Ask1p [Saccharomyces cerevisiae] [NCBI NR] gi\|486073\|emb\|CAA81888.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|433644\|emb\|CAA53419.1\| E292 [Saccharomyces cerevisiae] [NCBI NR] gi\|1587566\|prf\|\|2206495Q ORF [NCBI NR] (P35734) Hypothetical 32.1 kDa protein in NUP120-CSE4 intergenic region [Swiss-Prot] DASH complex subunit ASK1 - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] ASK1 Outer kinetochore protein - part of Dam1 complex [mips] Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases [SGD] ASK1, Chr XI from 339310-340188, reverse complement [yeast_orfs2] Chr XI from 339310-340188, reverse complement [yeastorf3.fasta] Chr XI from 339309-340187, reverse complement [yeastorf4.fasta] Chr XI from 340187-339309, reverse complement [yeastorf5.fasta] ASK1 SGDID:S0001535, Chr XI from 340191-339313, reverse complement, Verified ORF [yeastflysequest.fasta] Ask1p [Saccharomyces cerevisiae]gi\|51472787\|tpg\|DAA01815.1\| TPA: Ask1p [Saccharomyces cerevisiae]gi\|433644\|emb\|CAA53419.1\| E292 [Saccharomyces cerevisiae]gi\|486073\|emb\|CAA81888.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|539164\|pir\|\|S37874 [nrdb_11152004_Chlamydomonas] ASK1 SGDID:S000001535, Chr XI from 340190-339312, reverse complement, Verified ORF, "Component of the DASH complex that binds to microtubules and kinetochores and is essential for chromosome segregation; phosphorylated during the cell cycle by cyclin-depe [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ASK1 SGDID:S000001535, Chr XI from 340190-339312, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phos [yeast-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DASH complex subunit ASK1 (Outer kinetochore protein ASK1) (Associated with spindles and kinetochores protein 1) [nr-271106-contam.fasta] TPA_exp: Ask1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORF [nr-271106-contam.fasta] Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; Ask1p [Saccharomyces ce [nr-271106-contam.fasta] E292 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ASK1 SGDID:S000001535, Chr XI from 340190-339312, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases" [yeast-contam_custom.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	292 amino acids
Gene Ontology:
Cellular Component:	condensed nuclear chromosome, centromeric region [IDA] DASH complex [IDA ][IPI ] spindle [IDA] condensed nuclear chromosome kinetochore [IDA]
Biological Process:	mitotic spindle organization in nucleus [IMP] regulation of microtubule polymerization or depolymerization [IDA][IPI]
Molecular Function:	structural constituent of cytoskeleton [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSASKEETL EKLVQEITVN LQKIDSNLSF CFHKITQDII PHVATYSEIC ERIMDSTEWL  60
   61 GTMFQETGLV NLQANAAAPV GNAPVKSLVS NNVGIFPTSA EEASRQSQTD NGPNEADSAV 120
  121 HVNRDVHSMF NNDSIDDFHT ANITSTGQIL KLPDSSDEDT GSEAVPSREQ TDLTGEGHGG 180
  181 ADDEQDESTI QRQSRKRKIS LLLQQQYGSS SSMVPSPIVP NKMRKQLAHE EHINNDGDND 240
  241 DENSNNIESS PLKQGHHHPK GQADDNNEGP DEEESTKEVP KPGTIIHFST NR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ASK1 DAD1 DAD2 DAM1 DUO1 SPC19 SPC34
View Details	(MIPS) Mewes HW, et al. (2004)		ASK1 DAD1 DAD2 DAD3 DAD4 DAM1 DUO1 SPC19 SPC34
View Details	Krogan NJ, et al. (2006)		ASK1 DAD1 DAD4 DAM1 DUO1 HSK3 NCA2 SPC19 SPC34
View Details	Qiu et al. (2008)		ASK1 DAD1 DAD2 DAD3 DAD4 DAM1 DUO1 HSK3 SPC19 SPC25 SPC34

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	No Comments	Cheeseman IM, et al. (2002)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GBP2	ASK1	1	Unpublished Fields Lab Data
View Screen	YLL037W	ASK1	1	Hazbun TR, et al. (2003)
View Screen	PAM18	ASK1	1	Unpublished Fields Lab Data
View Screen	HOF1	ASK1	1	Unpublished Fields Lab Data
View Screen	UBC11	ASK1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ASK1	Huh WK, et al. (2003)
View Data	ASK1 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..96]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[97..233]	MSA	1.003974	View MSA. No confident structure predictions are available.
3	View Details	[234..292]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2