Protein Overview: NFU1

General Information:
Name(s) found:	NFU1 / YKL040C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322811\|ref\|NP_012884.1\| Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria; Nfu1p [Saccharomyces cerevisiae] [NCBI NR] gi\|549668\|sp\|P32860.2\|NFU1_YEAST RecName: Full=NifU-like protein, mitochondrial; Flags: Precursor [NCBI NR] pir\|\|S37861 nitrogen fixation protein nifU homolog YKL040c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486050\|emb\|CAA81875.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|45269699\|gb\|AAS56230.1\| YKL040C [Saccharomyces cerevisiae] [NCBI NR] NifU-like protein, mitochondrial OS=Saccharomyces cerevisiae GN=NFU1 PE=1 SV=2 [Swiss-Prot] NFU1 Iron homeostasis [mips] Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria [SGD] NFU1, Chr XI from 360698-361468, reverse complement [yeast_orfs2] Chr XI from 360698-361468, reverse complement [yeastorf3.fasta] Chr XI from 361468-360698, reverse complement [yeastorf5.fasta] (P32860) NifU-like protein [4932.FASTACCERV.txt] NFU1 SGDID:S0001523, Chr XI from 361472-360702, reverse complement, Verified ORF [yeastflysequest.fasta] Nfu1p [Saccharomyces cerevisiae]gi\|486050\|emb\|CAA81875.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|539300\|pir\|\|S37861 nitrogen fixation protein nifU homolog YKL040c - yeast (Saccharomyces cerevisiae)gi\|45269699\|gb\|AAS56230.1\| YKL040C [Sacc [nrdb_11152004_Chlamydomonas] NFU1 SGDID:S000001523, Chr XI from 361471-360701, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixin [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YKL040C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NifU-like protein, mitochondrial precursor [nr-271106-contam.fasta] Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria; Nfu1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NFU1 SGDID:S000001523, Chr XI from 361471-360701, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" [yeast-bovrabprots.fasta] [chemostatdb.fasta] NifU-like protein, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NFU1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NFU1 SGDID:S000001523, Chr XI from 361828-361058, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" [yeast-030211-contam.fasta] NFU1 SGDID:S000001523, Chr XI from 361828-361058, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" [20141208_orf_trans.fasta] YKL040C SGDID:S000001523, Chr XI from 361828-361058, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	256 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [IDA] mitochondrion [IDA]
Biological Process:	cellular iron ion homeostasis [IMP][ISS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFKSVAKLGK SPIFYLNSQR LIHIKTLTTP NENALKFLST DGEMLQTRGS KSIVIKNTDE  60
   61 NLINHSKLAQ QIFLQCPGVE SLMIGDDFLT INKDRMVHWN SIKPEIIDLL TKQLAYGEDV 120
  121 ISKEFHAVQE EEGEGGYKIN MPKFELTEED EEVSELIEEL IDTRIRPAIL EDGGDIDYRG 180
  181 WDPKTGTVYL RLQGACTSCS SSEVTLKYGI ESMLKHYVDE VKEVIQIMDP EQEIALKEFD 240
  241 KLEKKLESSK NTSHEK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ISA1 NFU1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CTF13	NFU1	1	Unpublished Fields Lab Data
View Screen	RAD51	NFU1	1	Unpublished Fields Lab Data
View Screen	CFD1	NFU1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NFU1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..145]	MSA	2.07398	View MSA. Confident ab initio structure predictions are available.
2	View Details	[146..256]	Pfam	40.091515	NifU-like domain No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
catalytic activity	0.60409960326459	bayes_pls_golite062009

Term	Confidence	Notes
binding	1.71550813147686	bayes_pls_golite062009
2 iron, 2 sulfur cluster binding	1.15693786817459	bayes_pls_golite062009
iron-sulfur cluster binding	0.692336376163639	bayes_pls_golite062009
metal cluster binding	0.692336376163639	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2