Protein Overview: MCM22

General Information:
Name(s) found:	MCM22 / YJR135C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322595\|ref\|NP_012669.1\| Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner; Mcm22p [Saccharomyces cerevisiae] [NCBI NR] gi\|1352932\|sp\|P47167.1\|MCM22_YEAST RecName: Full=Central kinetochore subunit MCM22; AltName: Full=Minichromosome maintenance protein 22 [NCBI NR] pir\|\|S57158 hypothetical protein YJR135c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1015872\|emb\|CAA89666.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P47167) Hypothetical 27.6 kDa protein in NMD5-HOM6 intergenic region [Swiss-Prot] Central kinetochore subunit MCM22 OS=Saccharomyces cerevisiae GN=MCM22 PE=1 SV=1 [Swiss-Prot] MCM22 Outer kinetochore protein [mips] Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner [SGD] MCM22, Chr X from 675752-676471, reverse complement [yeast_orfs2] Chr X from 675752-676471, reverse complement [yeastorf3.fasta] Chr X from 675753-676472, reverse complement [yeastorf4.fasta] Chr X from 676473-675754, reverse complement [yeastorf5.fasta] MCM22 SGDID:S0003896, Chr X from 676693-675974, reverse complement, Verified ORF [yeastflysequest.fasta] Mcm22p [Saccharomyces cerevisiae]gi\|1015872\|emb\|CAA89666.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1077917\|pir\|\|S57158 hypothetical protein YJR135c - yeast (Saccharomyces cerevisiae)gi\|1352932\|sp\|P47167\|YJ9D_YEAST Hypothetical 27.6 kDa pr [nrdb_11152004_Chlamydomonas] MCM22 SGDID:S000003896, Chr X from 676693-675974, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MCM22 SGDID:S000003896, Chr X from 676772-676053, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [yeast-contam.fasta] MCM22 SGDID:S000003896, Chr X from 676769-676050, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] MCM22 SGDID:S000003896, Chr X from 676768-676049, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [yeast-200209-contam.fasta] Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner; Mcm22p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Central kinetochore subunit MCM22 (Minichromosome maintenance protein 22) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Central kinetochore subunit MCM22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MCM22 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MCM22 SGDID:S000003896, Chr X from 676778-676059, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [yeast-030211-contam.fasta] MCM22 SGDID:S000003896, Chr X from 676778-676059, Genome Release 64-2-1, reverse complement, Verified ORF, "Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4" [20141208_orf_trans.fasta] TR1821\|c0_g1_i1\|g.1311 ORF TR1821\|c0_g1_i1\|g.1311 TR1821\|c0_g1_i1\|m.1311 type:complete len:240 (+) TR1821\|c0_g1_i1:81-800(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YJR135C SGDID:S000003896, Chr X from 676778-676059, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in minichromosome maintenance; component of the kinetochore; binds to centromeric DNA in a Ctf19p-dependent manner" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5452\|g.4008 ORF asmbl_5452\|g.4008 asmbl_5452\|m.4008 type:complete len:240 (+) asmbl_5452:97-816(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5454\|g.3514 ORF ID=asmbl_5454\|g.3514 ID=asmbl_5454\|m.3514 type:complete len:240 (+) ID=asmbl_5454:97-816(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	239 amino acids
Gene Ontology:
Cellular Component:	condensed nuclear chromosome kinetochore [IDA]
Biological Process:	chromosome segregation [IPI]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDVEKDVLDV YIKNLENQIG NKRYFLKQAQ GAIDEITKRS LDTEGKPVNS EVFTELLRKP  60
   61 MFFSERADPI GFSLTSNFLS LRAQSSSEWL SLMNDQSVDQ KAMLLLQNNI NSDLKELLRK 120
  121 LQHQMTIMDS KKQDHAHIRT RKARNKELWD SLADFLKGYL VPNLDDNDES IDSLTNEVML 180
  181 LMKRLIEHDL NLTLNDFSSK TIPIYRLLLR ANIITVIEGS TNPGTKYIKL IDFNETSLT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		AME1 CHL4 CTF19 CTF3 IML3 MCM21 MCM22 NKP1 NKP2 OKP1 PSH1
View Details	Hazbun TR, et al. (2003)		AME1 CHL4 CTF19 CTF3 DSN1 MCM22 MTW1 NKP1 NNF1 NSL1 OKP1
View Details	(MIPS) Mewes HW, et al. (2004)		CTF3 MCM16 MCM22
View Details	Krogan NJ, et al. (2006)		AME1 ARG5,6 CHL4 CTF19 CTF3 IML3 MCM16 MCM21 MCM22 NKP1 NKP2 OKP1 PRP18 SET4 YNR062C
View Details	Gavin AC, et al. (2006)		CHL4 CTF19 CTF3 MCM21 MCM22 OKP1
View Details	Gavin AC, et al. (2002)		AME1 CHL4 CTF19 CTF3 IML3 MCM21 MCM22 NKP1
View Details	Qiu et al. (2008)		MCM16 MCM22

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CHL4	No Comments	Cheeseman IM, et al. (2002)
View Run	MCM16	No Comments	Cheeseman IM, et al. (2002)
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CTF3	MCM22	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MCM22	Huh WK, et al. (2003)
View Data	MCM22 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..239]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2