Protein Overview: GGA2

General Information:
Name(s) found:	GGA2 / YHR108W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731696\|sp\|P38817.1\|GGA2_YEAST RecName: Full=ADP-ribosylation factor-binding protein GGA2; AltName: Full=Golgi-localized, gamma ear-containing, ARF-binding protein 2 [NCBI NR] gi\|6321900\|ref\|NP_011976.1\| Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; Gga2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48950 hypothetical protein YHR108w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|529121\|gb\|AAB68854.1\| Yhr108wp [Saccharomyces cerevisiae] [NCBI NR] ADP-ribosylation factor-binding protein GGA2 OS=Saccharomyces cerevisiae GN=GGA2 PE=1 SV=1 [Swiss-Prot] GGA2 Arf-binding protein [mips] Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi [SGD] GGA2, Chr VIII from 328305-330062 [yeast_orfs2] Chr VIII from 328305-330062 [yeastorf3.fasta] (P38817) ADP-ribosylation factor binding protein GGA2 (Golgi-localized, gamma ear-containing, ARF-binding protein 2) [4932.FASTACCERV.txt] GGA2 SGDID:S0001150, Chr VIII from 328305-330062, Verified ORF [yeastflysequest.fasta] Gga2p [Saccharomyces cerevisiae]gi\|731696\|sp\|P38817\|GGA2_YEAST ADP-ribosylation factor binding protein GGA2 (Golgi-localized, gamma ear-containing, ARF-binding protein 2)gi\|529121\|gb\|AAB68854.1\| Yhr108wp [Saccharomyces cerevisiae]gi\|630123\|pir\|\|S48950 [nrdb_11152004_Chlamydomonas] GGA2 SGDID:S000001150, Chr VIII from 328306-330063, Verified ORF, "Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; Gga2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ADP-ribosylation factor-binding protein GGA2 (Golgi-localized, gamma ear-containing, ARF-binding protein 2) [nr-271106-contam.fasta] Yhr108wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] ADP-ribosylation factor-binding protein GGA2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GGA2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GGA2 SGDID:S000001150, Chr VIII from 328303-330060, Genome Release 64-1-1, Verified ORF, "Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin" [yeast-030211-contam.fasta] GGA2 SGDID:S000001150, Chr VIII from 328303-330060, Genome Release 64-2-1, Verified ORF, "Protein that regulates Arf1p, Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YHR108W SGDID:S000001150, Chr VIII from 328303-330060, Genome Release 64-1-1, Verified ORF, "Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	585 amino acids
Gene Ontology:
Cellular Component:	trans-Golgi network [IDA]
Biological Process:	protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway [IMP] Golgi to endosome transport [IMP] Golgi to vacuole transport [IMP][IPI]
Molecular Function:	ubiquitin binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSHPHSHSIY LSELPVRKPQ ALGNPLLRKI QRACRMSLAE PDLALNLDIA DYINEKQGAA  60
   61 PRDAAIALAK LINNRESHVA IFALSLLDVL VKNCGYPFHL QISRKEFLNE LVKRFPGHPP 120
  121 LRYSKIQRLI LTAIEEWYQT ICKHSSYKND MGYIRDMHRL LKYKGYAFPK ISESDLAVLK 180
  181 PSNQLKTASE IQKEQEIAQA AKLEELIRRG KPEDLREANK LMKIMAGFKE DNAVQAKQAI 240
  241 SSELNKLKRK ADLLNEMLES PDSQNWDNET TQELHSALKV AQPKFQKIIE EEQEDDALVQ 300
  301 DLLKFNDTVN QLLEKFNLLK NGDSNAASQI HPSHVSAPLQ QSSGALTNEI NLIDFNDLDE 360
  361 APSQGNNNTN GTGTPAAAET SVNDLLGDLT DLSISNPSTA NQASFGLGGD IVLGSSQPAP 420
  421 PVTTTNNSNN TLDLLGLSTP QSPTNSQAVN SSGFDLLMGF NPTTGTTTAP ARTLVNQSPN 480
  481 LKIEFEISRE SNSVIRIKSF FTNLSSSPIS NLVFLLAVPK SMSLKLQPQS SNFMIGNAKD 540
  541 GISQEGTIEN APANPSKALK VKWKVNYSVN STQAEETAVF TLPNV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		CHC1 GCN1 GGA2 LYS12
View Details	Qiu et al. (2008)		GGA1 GGA2

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ARF3	GGA2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GGA2	Huh WK, et al. (2003)
View Data	GGA2 and CHC1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..96]	PSI-BLAST	177.626727	Tom1 protein
2	View Details	[97..196]	PSI-BLAST	177.626727	Tom1 protein
3	View Details	[197..337]	Pfam	3.0	GAT domain No confident structure predictions are available.
4	View Details	[338..445]	deduced	N/A	No confident structure predictions are available.
5	View Details	[446..585]	PSI-BLAST	36.522879	Gamma1-adaptin domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
clathrin binding	5.9355718186759	bayes_pls_golite062009
binding	2.36215929275587	bayes_pls_golite062009
phosphoinositide binding	1.69886980741044	bayes_pls_golite062009
protein binding	1.55859524125292	bayes_pls_golite062009
phospholipid binding	1.35873451348399	bayes_pls_golite062009
lipid binding	0.681936854618139	bayes_pls_golite062009
phosphatidylinositol-4,5-bisphosphate binding	0.2359206178887	bayes_pls_golite062009
phosphatidylinositol binding	0.10007172207713	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	1.93554535044651	bayes_pls_golite062009
protein binding	1.17960228368657	bayes_pls_golite062009
transporter activity	0.169557557237959	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.85

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2