Protein Overview: YGR125W

General Information:
Name(s) found:	YGR125W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|642349\|emb\|CAA58161.1\| G6362 [Saccharomyces cerevisiae] [NCBI NR] gi\|6321564\|ref\|NP_011641.1\| Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; Ygr125wp [Saccharomyces cerevisi [NCBI NR] gi\|1723705\|sp\|P53273.1\|YG35_YEAST RecName: Full=Uncharacterized vacuolar membrane protein YGR125W [NCBI NR] gi\|1323205\|emb\|CAA97136.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S55984 probable membrane protein YGR125w - yeast (Saccharomyces cerevisiae) [NCBI NR] Uncharacterized vacuolar membrane protein YGR125W OS=Saccharomyces cerevisiae GN=YGR125W PE=1 SV=1 [Swiss-Prot] Protein of unknown function localised to vacuole [mips] Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole [SGD] YGR125W, Chr VII from 742320-745430 [yeast_orfs2] Chr VII from 742320-745430 [yeastorf3.fasta] Chr VII from 742327-745437 [yeastorf4.fasta] (P53273) Hypothetical 117.0 kDa protein in ASN2-PHB1 intergenic region [4932.FASTACCERV.txt] YGR125W SGDID:S0003357, Chr VII from 742328-745438, Uncharacterized ORF [yeastflysequest.fasta] YGR125W SGDID:S0003357, Chr VII from 742330-745440, Uncharacterized ORF [10112005cervSGD.fasta] Ygr125wp [Saccharomyces cerevisiae]gi\|1323205\|emb\|CAA97136.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|642349\|emb\|CAA58161.1\| G6362 [Saccharomyces cerevisiae]gi\|1084830\|pir\|\|S55984 probable membrane protein YGR125w - yeast (Saccharomyces ce [nrdb_11152004_Chlamydomonas] YGR125W SGDID:S000003357, Chr VII from 742330-745440, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YGR125W SGDID:S000003357, Chr VII from 742330-745440, Uncharacterized ORF, "Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes [yeast-contam.fasta] G6362 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized vacuolar membrane protein YGR125W [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; Ygr125wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YGR125W SGDID:S000003357, Chr VII from 742330-745440, Uncharacterized ORF, "Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Uncharacterized vacuolar membrane protein YGR125W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YGR125W PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YGR125W SGDID:S000003357, Chr VII from 742325-745435, Genome Release 64-1-1, Uncharacterized ORF, "Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole" [yeast-030211-contam.fasta] YGR125W SGDID:S000003357, Chr VII from 742325-745435, Genome Release 64-2-1, Uncharacterized ORF, "Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole" [20141208_orf_trans.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1036 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole [IDA]
Biological Process:	biological_process [ND]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM SGFFTGGNSP  60
   61 MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS SSVEENSPTI KDYMDIIGNG 120
  121 DRKDDQSMRT IEENIDEEYS DEYSRLLLSP ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ 180
  181 SLRPSHNLRF RPRNLWHMCT SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS 240
  241 HLGPTGISMF YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE 300
  301 IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF LIITGIEVTT 360
  361 RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR YFKNSLVLPS FYILTLVLFH 420
  421 FIVAIIPTLS LDALRQAGWI FPIANSDSKW YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF 480
  481 GILHVPINVP ALAMSLQMDK YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA 540
  541 DSPFAGFLLI ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY 600
  601 LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR STVYRDYVQT 660
  661 KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK DATKRRIKYL ILDFKNINAD 720
  721 NIDYSAAEGF NRIKRFTETK RIKLIISSIK ERDRIYNAFN NVGLLNDVEL FADLNSALEW 780
  781 CENEFLFQYK QLRKKAKERL EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL 840
  841 MSLPTNTPRN YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV 900
  901 QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL PQGTLYEIFS 960
  961 NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN KLKEDNLALY VEVALMVMCI1020
 1021 KDTRFKELLG YTLVSA

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SNF1	YGR125W	1	Unpublished Fields Lab Data
View Screen	RAD54	YGR125W	1	Unpublished Fields Lab Data
View Screen	MPE1	YGR125W	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	YGR125W	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..76]	deduced	N/A	No confident structure predictions are available.
2	View Details	[77..202]	PSI-BLAST	6.154902	Erythrocite membrane Band 3
3	View Details	[203..524]	PSI-BLAST	3.69897	No description for 2yxqA was found.
4	View Details	[525..644]	MSA	3.998995	View MSA. No confident structure predictions are available.
5	View Details	[645..766]	PSI-BLAST	5.154902	Solution Structure of putative anti sigma factor antagonist from Thermotoga maritima (TM1442)
6	View Details	[767..880]	deduced	N/A	No confident structure predictions are available.
7	View Details	[881..1036]	FFAS03	4.28	No description for 2pqqA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2