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Protein Overview: NNF2

General Information:
Name(s) found: NNF2 / YGR089W [SGD]
Description(s) found:
Found 27 descriptions.
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  • Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 936 amino acids

    Gene Ontology:
    Cellular Component: membrane fraction [IDALink to PubMed article]
    Biological Process: chromosome segregation [RCALink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEEQFTNQKK VSHLQSLMNT KRSEQPTEFA KKHRFKDTLA LFLVFLSFNH FTSLCLLVSF  60
   61 IVATKCKDFL ANCFIILFLS KKPSRHIGEV AHIDISTSKV TNGSSNRKSN SRFFGNSKNS 120
  121 FVIPIPVLIC EILFAMLLKI YGGDYFVKPI KNLAISIIAS FLINDPSDCL SYATSCSVLY 180
  181 AVSTNTFQRV SHFFDIIQLF DMSLRGTGQS IKLFTVFRKY SQFFKKLFSL FLPMPFKMLG 240
  241 KHSDSMIYYL SFHILFFSFA SSLLHPHRQT AENKPLKKGF NSTKPDVARV QGLQKMRISS 300
  301 SSSVSADSNT LEDQSPMIPN DPGGLSSSNQ TIHPSQQNNS PVPLSSHSNI LNPAASYPTD 360
  361 ATSSFPYFTS MVKEYKSYQP SVISAEGSNS QAVTTTTSTT TSPTTFNFSG DNTSLSNEIS 420
  421 LSDSSNGNSK KDSDFFSPSN DKYTNQLFEL NVDFGNMFSS SKLSSDISVT SNLENFIRLL 480
  481 FRRKNQHLIA PLWSMVVTLK TTNFEKKYLQ ETSENSLTPT NSNTSYVSNQ EKHDKDLDTI 540
  541 NTHSVSSRIS FTHAGKFKKS VFNNFEPSNT MALIAKTTSD DYNLLNLVST NENIFNRNDN 600
  601 DYKVCIIDIS TNSITFHIEN LHDGELIVLV NGVIWSEVSC ALILEHVGEE YVVVNGLVPS 660
  661 CSYDIQFINR LNHRDDYLVS DLIVRTCGNN NAIAGKFENL DFSFPSYYHR KFLSPLLTLK 720
  721 HSVLTTNANL SDERTKLKKT KKEFSKKLSL LRQEIDYFKG RISQNATHDE KSTLKVENLK 780
  781 VALQQSETAV NKLEMQLKTL TEKELELEEE YLKKKDLHLK NQLEFSKLEE SLSKDLKNSE 840
  841 GRFQKVNQEL VQLGSKLDKL NARNEKLQKE VDQNAEEIEK FSTQFLSKRE KDRFRRKEYR 900
  901 IREANKFELT IKGLEQDINR LENENENIHS LIGNSY

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data NNF2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..208] deduced N/A No confident structure predictions are available.
    2 View Details [209..589] MSA 13.437997 View MSA. No confident structure predictions are available.
    3 View Details [590..657] deduced N/A No confident structure predictions are available.
    4 View Details [658..711] PSI-BLAST 53.69897 Tropomyosin
    5 View Details [712..789] PSI-BLAST 53.69897 Tropomyosin
    6 View Details [790..852] PSI-BLAST 53.69897 Tropomyosin
    7 View Details [853..936] PSI-BLAST 53.69897 Tropomyosin

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • binding
    		•  1.00940588691795 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.516346942965208 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.513833930010015 bayes_pls_golite062009
  • DNA binding
    		•  0.475884563138359 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle