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Protein Overview: PEF1

General Information:
Name(s) found: PEF1 / YGR058W [SGD]
Description(s) found:
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  • gi|6321495|ref|NP_011572.1| Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; Pef1p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S64352 hypothetical protein YGR058w - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1723672|sp|P53238.1|CPNSH_YEAST RecName: Full=Calpain small subunit homolog protein YGR058W [NCBI NR]
  • gi|1323073|emb|CAA97059.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • (P53238) Hypothetical 38.4 kDa protein in MUP1-SPR3 intergenic region [Swiss-Prot]
  • Peflin OS=Saccharomyces cerevisiae GN=PEF1 PE=1 SV=1 [Swiss-Prot]
  • Calpain small subunit homolog protein YGR058W OS=Saccharomyces cerevisiae GN=YGR058W PE=1 SV=1 [Swiss-Prot]
  • Protein of unknown function localised to cytoplasm and nucleus [mips]
  • Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2 [SGD]
  • YGR058W, Chr VII from 606130-607137 [yeast_orfs2]
  • Chr VII from 606130-607137 [yeastorf3.fasta]
  • Chr VII from 606137-607144 [yeastorf4.fasta]
  • YGR058W SGDID:S0003290, Chr VII from 606138-607145, Uncharacterized ORF [yeastflysequest.fasta]
  • YGR058W SGDID:S0003290, Chr VII from 606140-607147, Uncharacterized ORF [10112005cervSGD.fasta]
  • Ygr058wp [Saccharomyces cerevisiae]gi|1323073|emb|CAA97059.1| unnamed protein product [Saccharomyces cerevisiae]gi|2131660|pir||S64352 hypothetical protein YGR058w - yeast (Saccharomyces cerevisiae)gi|1723672|sp|P53238|YG25_YEAST Hypothetical 38.4 kDa [nrdb_11152004_Chlamydomonas]
  • YGR058W SGDID:S000003290, Chr VII from 606140-607147, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • YGR058W SGDID:S000003290, Chr VII from 606140-607147, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus" [yeast-contam.fasta]
  • PEF1 SGDID:S000003290, Chr VII from 606140-607147, Verified ORF, "Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • Calpain small subunit homolog protein YGR058W [nr-271106-contam.fasta]
  • Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; Ygr058wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 335 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    Biological Process: biological_process [ND]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCAKKLKYAA GDDFVRYATP KEAMEETRRE FEKEKQRQQQ IKVTQAQTPN TRVHSAPIPL  60
   61 QTQYNKNRAE NGHHSYGSPQ SYSPRHTKTP VDPRYNVIAQ KPAGRPIPPA PTHYNNLNTS 120
  121 AQRIASSPPP LIHNQAVPAQ LLKKVAPASF DSREDVRDMQ VATQLFHNHD VKGKNRLTAE 180
  181 ELQNLLQNDD NSHFCISSVD ALINLFGASR FGTVNQAEFI ALYKRVKSWR KVYVDNDING 240
  241 SLTISVSEFH NSLQELGYLI PFEVSEKTFD QYAEFINRNG TGKELKFDKF VEALVWLMRL 300
  301 TKLFRKFDTN QEGIATIQYK DFIYATLYLG RFLPH

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen LSM8 PEF1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data PEF1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..160] PSI-BLAST 106.519517 Sorcin
    2 View Details [161..245] PSI-BLAST 106.519517 Sorcin
    3 View Details [246..335] PSI-BLAST 179.68867 Apoptosis-linked protein alg-2

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • calcium ion binding
    		•  3.79609619542776 bayes_pls_golite062009
  • binding
    		•  3.17556402087346 bayes_pls_golite062009
  • protein binding
    		•  2.21291432166051 bayes_pls_golite062009
  • cation binding
    		•  1.58747127156827 bayes_pls_golite062009
  • metal ion binding
    		•  1.58747127156827 bayes_pls_golite062009
  • ion binding
    		•  1.57990289083749 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.450279748770607 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.0981928858355918 bayes_pls_golite062009
  • DNA binding
    		•  0.0960817636106102 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle