Protein Overview: YEL047C

General Information:
Name(s) found:	YEL047C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320788\|ref\|NP_010867.1\| Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throu [NCBI NR] gi\|603632\|gb\|AAB64995.1\| Yel047cp [Saccharomyces cerevisiae] [NCBI NR] gi\|418423\|sp\|P32614.1\|FRDS_YEAST RecName: Full=Fumarate reductase; AltName: Full=NADH; AltName: Full=NADH-dependent fumarate reductase; AltName: Full=FAD-dependent oxidoreductase FRDS [NCBI NR] pir\|\|S30830 fumarate reductase (EC 1.3.99.1) - yeast (Saccharomyces cerevisiae) [NCBI NR] (P32614) Hypothetical FAD-dependent oxidoreductase YEL047C [Swiss-Prot] Fumarate reductase OS=Saccharomyces cerevisiae GN=YEL047C PE=1 SV=1 [Swiss-Prot] Soluble fumarate reductase, cytoplasmic [mips] Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies [SGD] YEL047C, Chr V from 65385-66797, reverse complement [yeast_orfs2] Chr V from 65385-66797, reverse complement [yeastorf3.fasta] Chr V from 66797-65385, reverse complement [yeastorf5.fasta] YEL047C SGDID:S0000773, Chr V from 66797-65385, reverse complement, Uncharacterized ORF [yeastflysequest.fasta] Fumurate ReDuctase Soluble [Saccharomyces cerevisiae]gi\|418423\|sp\|P32614\|YEF7_YEAST Hypothetical FAD-dependent oxidoreductase YEL047Cgi\|320733\|pir\|\|S30830 fumarate reductase (EC 1.3.99.1) - yeast (Saccharomyces cerevisiae)gi\|603632\|gb\|AAB64995.1\| Yel04 [nrdb_11152004_Chlamydomonas] YEL047C SGDID:S000000773, Chr V from 66797-65385, reverse complement, Uncharacterized ORF, "Fumurate ReDuctase Soluble" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YEL047C SGDID:S000000773, Chr V from 66797-65385, reverse complement, Verified ORF, "Fumurate ReDuctase Soluble" [yeast-contam.fasta] YEL047C SGDID:S000000773, Chr V from 66797-65385, reverse complement, Verified ORF, "Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged p [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Fumarate reductase (NADH) (NADH-dependent fumarate reductase) (FAD-dependent oxidoreductase FRDS) [nr-271106-contam.fasta] Fumurate ReDuctase Soluble; Yel047cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Yel047cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YEL047C SGDID:S000000773, Chr V from 66797-65385, reverse complement, Verified ORF, "Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Fumarate reductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YEL047C PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YEL047C SGDID:S000000773, Chr V from 66797-65385, Genome Release 64-1-1, reverse complement, Verified ORF, "Soluble fumarate reductase, required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies" [yeast-030211-contam.fasta] FRD1 SGDID:S000000773, Chr V from 66797-65385, Genome Release 64-2-1, reverse complement, Verified ORF, "Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] Fumarate reductase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FRD1 PE=1 SV=1 [uniprot_sprot.fasta.20160308]
Organism:	Saccharomyces cerevisiae
Length:	470 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] cytosol [IDA] ribosome [IDA]
Biological Process:	metabolic process [TAS]
Molecular Function:	fumarate reductase (NADH) activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLSPVVVIG TGLAGLAAAN ELVNKYNIPV TILEKASSIG GNSIKASSGI NGACTETQRH  60
   61 FHIEDSPRLF EDDTIKSAKG KGVQELMAKL ANDSPLAIEW LKNEFDLKLD LLAQLGGHSV 120
  121 ARTHRSSGKL PPGFEIVSAL SNNLKKLAET KPELVKINLD SKVVDIHEKD GSISAVVYED 180
  181 KNGEKHMVSA NDVVFCSGGF GFSKEMLKEY APELVNLPTT NGQQTTGDGQ RLLQKLGADL 240
  241 IDMDQIQVHP TGFIDPNDRS SSWKFLAAES LRGLGGILLN PITGRRFVNE LTTRDVVTAA 300
  301 IQKVCPQEDN RALLVMGEKM YTDLKNNLDF YMFKKLVQKL TLSQVVSEYN LPITVAQLCE 360
  361 ELQTYSSFTT KADPLGRTVI LNEFGSDVTP ETVVFIGEVT PVVHFTMGGA RINVKAQVIG 420
  421 KNDERLLKGL YAAGEVSGGV HGANRLGGSS LLECVVFGRT AAESIANDRK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		SHM2 YEL047C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YKL036C	YEL047C	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	YEL047C	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..248] [403..470]	PSI-BLAST	635.045757	Flavocytochrome c3 (respiratory fumarate reductase), N-terminal domain; Flavocytochrome c3 (respiratory fumarate reductase)
2	View Details	[249..402]	PSI-BLAST	635.045757	Flavocytochrome c3 (respiratory fumarate reductase), N-terminal domain; Flavocytochrome c3 (respiratory fumarate reductase)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
succinate dehydrogenase activity	4.66525249592263	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor	3.70791057697947	bayes_pls_golite062009
succinate dehydrogenase (ubiquinone) activity	3.70791057697947	bayes_pls_golite062009
oxidoreductase activity	3.25470541847124	bayes_pls_golite062009
electron carrier activity	2.8553929424231	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	2.84060092432585	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	2.78793325009909	bayes_pls_golite062009
L-aspartate oxidase activity	2.52327499022066	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor	2.26549080531565	bayes_pls_golite062009
aspartate oxidase activity	2.16464783424214	bayes_pls_golite062009
oxidoreductase activity, acting on sulfur group of donors	2.11247286526577	bayes_pls_golite062009
catalytic activity	1.75556359035561	bayes_pls_golite062009
dihydrolipoyl dehydrogenase activity	1.73872135285388	bayes_pls_golite062009
binding	1.41440014472974	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor	0.932244769173724	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-NH2 group of donors	0.798505263716232	bayes_pls_golite062009
oxygen-dependent protoporphyrinogen oxidase activity	0.71497408952162	bayes_pls_golite062009
fumarate reductase (NADH) activity	0.65260793370113	bayes_pls_golite062009
succinate-CoA ligase activity	0.468246082354334	bayes_pls_golite062009
coenzyme binding	0.44036421821989	bayes_pls_golite062009
protein binding	0.159209309982354	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2