Protein Overview: YEF1

General Information:
Name(s) found:	YEF1 / YEL041W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320794\|ref\|NP_010873.1\| ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes; Yef1p [Saccharomyces cerevis [NCBI NR] gi\|603638\|gb\|AAB65001.1\| Yel041wp [Saccharomyces cerevisiae] [NCBI NR] gi\|418405\|sp\|P32622.1\|YEF1_YEAST RecName: Full=Uncharacterized kinase YEL041W [NCBI NR] pir\|\|S30838 hypothetical protein YEL041w - yeast (Saccharomyces cerevisiae) [NCBI NR] Uncharacterized kinase YEL041W OS=Saccharomyces cerevisiae GN=YEL041W PE=1 SV=1 [Swiss-Prot] YEF1 ATP-NADH kinase [mips] ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes [SGD] YEL041W, Chr V from 75944-77431 [yeast_orfs2] Chr V from 75944-77431 [yeastorf3.fasta] (P32622) Hypothetical 55.9 kDa protein in GDA1-UTR2 intergenic region [4932.FASTACCERV.txt] YEL041W SGDID:S0000767, Chr V from 75944-77431, Uncharacterized ORF [yeastflysequest.fasta] Yel041wp [Saccharomyces cerevisiae]gi\|418405\|sp\|P32622\|YEF1_YEAST Hypothetical 55.9 kDa protein in GDA1-UTR2 intergenic regiongi\|320711\|pir\|\|S30838 hypothetical protein YEL041w - yeast (Saccharomyces cerevisiae)gi\|603638\|gb\|AAB65001.1\| Yel041wp [Saccha [nrdb_11152004_Chlamydomonas] YEL041W SGDID:S000000767, Chr V from 75944-77431, Uncharacterized ORF, "Hypothetical protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Verified ORF, "ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenot [yeast-contam.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Verified ORF, "ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenoty [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Uncharacterized kinase YEL041W [nr-271106-contam.fasta] Yel041wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes; Yef1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Verified ORF, "ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes" [yeast-bovrabprots.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Verified ORF, "ATP-NADH kinase; phosophorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes" [yeast-contam_custom.fasta] [chemostatdb.fasta] ATP-NADH kinase YEF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YEF1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Genome Release 64-1-1, Verified ORF, "ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes" [yeast-030211-contam.fasta] YEF1 SGDID:S000000767, Chr V from 75944-77431, Genome Release 64-2-1, Verified ORF, "ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YEL041W SGDID:S000000767, Chr V from 75944-77431, Genome Release 64-1-1, Verified ORF, "ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; sequence similarity to Utr1p and Pos5p; overexpression complements certain pos5 phenotypes" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	495 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	NADP biosynthetic process [IDA]
Molecular Function:	NAD+ kinase activity [IDA] NADH kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKTDRLLINA SPETCTKGDA EMDTMDTIDR MTSVKVLAEG KVLSNFEEPG LMRCGYHDAK  60
   61 NWVRRLSSET IVGEDTSNLY PFYVDTAYDV RRLRKDLINA KVDLQVENLI IICNINDIST 120
  121 VFLMREVVEW ILRNFHSITV YVQDIFKKST QFAVGDLCKD SNCSKNRVKY WSKEFVKKHD 180
  181 SFFDLMITLG GDGTVLFASS IFTKDVPPIV PFALGSLGFL TNFEFQNFKE TLKHILTDEV 240
  241 RINLRMRLQC KLYRRNKPEI DAATGRKICY IDFISEHHVL NEVTIDRGPA PCLSLLELYG 300
  301 NDSLMTKVQG DGLIVATPTG STAYSLSAGG SLISPSVNAI AVTPICPHTL SFRPIILPDS 360
  361 MELKVRVDMN SRGTSWVNFD GKDRVELKQG DYVVITASPY SVPTIESSAS EFFESISKNL 420
  421 NWNDREEQKP FAHILSPKNQ EKYRLDSSKN GNDTISNPLE SSCISSDAQD EERKSVTETE 480
  481 TEIVVERTRQ AHFAI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		AVT1 DOA4 MPC54 YEF1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	DMA1	YEF1	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..99]	deduced	N/A	No confident structure predictions are available.
2	View Details	[100..443]	Pfam	138.387216	ATP-NAD kinase No confident structure predictions are available.
3	View Details	[444..495]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.89

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2