Protein Overview: ASP1

General Information:
Name(s) found:	ASP1 / YDR321W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|914995\|gb\|AAB64757.1\| Asp1p: Asparaginase I [Saccharomyces cerevisiae] [NCBI NR] gi\|728900\|sp\|P38986.1\|ASPG1_YEAST RecName: Full=L-asparaginase 1; AltName: Full=L-asparaginase I; AltName: Full=L-asparagine amidohydrolase I; Short=ASP I [NCBI NR] gi\|6320527\|ref\|NP_010607.1\| Cytosolic L-asparaginase, involved in asparagine catabolism; Asp1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48513 asparaginase (EC 3.5.1.1), cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|510198\|emb\|CAA81794.1\| intracellular isozyme of L-asparaginase [Saccharomyces cerevisiae] [NCBI NR] L-asparaginase 1 OS=Saccharomyces cerevisiae GN=ASP1 PE=1 SV=1 [Swiss-Prot] (P38986) L-asparaginase I (EC 3.5.1.1) (L-asparagine amidohydrolase I) (ASP I) [Swiss-Prot] ASP1 Asparaginase [mips] Cytosolic L-asparaginase, involved in asparagine catabolism [SGD] ASP1, Chr IV from 1108694-1109839 [yeast_orfs2] Chr IV from 1108694-1109839 [yeastorf3.fasta] ASP1 SGDID:S0002729, Chr IV from 1108695-1109840, Verified ORF [yeastflysequest.fasta] ASP1 SGDID:S0002729, Chr IV from 1108697-1109842, Verified ORF [10112005cervSGD.fasta] Asp1p [Saccharomyces cerevisiae]gi\|914995\|gb\|AAB64757.1\| Asp1p: Asparaginase I [Saccharomyces cerevisiae]gi\|510198\|emb\|CAA81794.1\| intracellular isozyme of L-asparaginase [Saccharomyces cerevisiae]gi\|728900\|sp\|P38986\|ASG1_YEAST L-asparaginase I (L-aspa [nrdb_11152004_Chlamydomonas] ASP1 SGDID:S000002729, Chr IV from 1108697-1109842, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ASP1 SGDID:S000002729, Chr IV from 1108699-1109844, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism" [yeast-contam.fasta] ASP1 SGDID:S000002729, Chr IV from 1108700-1109845, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Cytosolic L-asparaginase, involved in asparagine catabolism; Asp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Asp1p: Asparaginase I [Saccharomyces cerevisiae] [nr-271106-contam.fasta] L-asparaginase 1 (L-asparaginase I) (L-asparagine amidohydrolase I) (ASP I) [nr-271106-contam.fasta] intracellular isozyme of L-asparaginase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] L-asparaginase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ASP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ASP1 SGDID:S000002729, Chr IV from 1108702-1109847, Genome Release 64-1-1, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism" [yeast-030211-contam.fasta] ASP1 SGDID:S000002729, Chr IV from 1108702-1109847, Genome Release 64-2-1, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively" [20141208_orf_trans.fasta] TR1532\|c0_g1_i1\|g.1084 ORF TR1532\|c0_g1_i1\|g.1084 TR1532\|c0_g1_i1\|m.1084 type:complete len:382 (+) TR1532\|c0_g1_i1:104-1249(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDR321W SGDID:S000002729, Chr IV from 1108702-1109847, Genome Release 64-1-1, Verified ORF, "Cytosolic L-asparaginase, involved in asparagine catabolism" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_158\|g.211 ORF ID=asmbl_158\|g.211 ID=asmbl_158\|m.211 type:complete len:382 (+) ID=asmbl_158:57-1202(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	381 amino acids
Gene Ontology:
Cellular Component:	cytosol [TAS] intracellular [IDA]
Biological Process:	asparagine catabolic process [IGI][IMP]
Molecular Function:	asparaginase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKSDSVEITT ICPDVENSQF VVQSNCPETI PEILKSQNAA VNGSGIACQQ RSLPRIKILG  60
   61 TGGTIASKAI DSSQTAGYHV DLTIQDLLDA IPDISKVCDI EYEQLCNVDS KDINEDILYK 120
  121 IYKGVSESLQ AFDGIVITHG TDTLSETAFF IESTIDAGDV PIVFVGSMRP STSVSADGPM 180
  181 NLYQAICIAS NPKSRGRGVL VSLNDQISSG YYITKTNANS LDSFNVRQGY LGNFVNNEIH 240
  241 YYYPPVKPQG CHKFKLRVDG KHFKLPEVCI LYAHQAFPPA IVNLVADKYD GIVLATMGAG 300
  301 SLPEEVNETC MKLSLPIVYS KRSMDGMVPI ANVPKKGSKE DNLIASGYLS PEKSRILLQL 360
  361 CLAGNYTLEE IKHVFTGVYG G

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HMT1	ASP1	1	Unpublished Fields Lab Data
View Screen	LIA1	ASP1	1	Unpublished Fields Lab Data
View Screen	NSE5	ASP1	1	Hazbun TR, et al. (2003)
View Screen	RRB1	ASP1	1	Unpublished Fields Lab Data
View Screen	NAF1	ASP1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..264]	PSI-BLAST	609.9897	Asparaginase type II
2	View Details	[265..381]	PSI-BLAST	609.9897	Asparaginase type II

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
asparaginase activity	5.31552786314061	bayes_pls_golite062009
hydrolase activity	4.24082880889802	bayes_pls_golite062009
catalytic activity	1.35076351157997	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.739997194025722	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	0.584091474722373	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2