Protein Overview: WHI4

General Information:
Name(s) found:	WHI4 / YDL224C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74676444\|sp\|Q07655.1\|WHI4_YEAST RecName: Full=Protein WHI4 [NCBI NR] gi\|6319977\|ref\|NP_010057.1\| Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division; Whi4p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S67787 hypothetical protein YDL224c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1431377\|emb\|CAA98803.1\| WHI4 [Saccharomyces cerevisiae] [NCBI NR] Protein WHI4 OS=Saccharomyces cerevisiae GN=WHI4 PE=1 SV=1 [Swiss-Prot] WHI4 Protein regulates the cell size requirement for passage through Start and commitment to cell division [mips] Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division [SGD] WHI4, Chr IV from 54398-56347, reverse complement [yeast_orfs2] Chr IV from 54398-56347, reverse complement [yeastorf3.fasta] Chr IV from 56347-54398, reverse complement [yeastorf5.fasta] >Q07655 (Q07655) Chromosome IV reading frame ORF YDL224C [4932.FASTACCERV.txt] WHI4 SGDID:S0002383, Chr IV from 56347-54398, reverse complement, Verified ORF [yeastflysequest.fasta] Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division [Saccharomyces cerevisiae]gi\|1431377\|emb\|CAA98803.1\| WHI4 [Saccharomyces cerevisiae]gi\| [nrdb_11152004_Chlamydomonas] (Q07655) WHI4 protein [cerv4932.FASTAC] WHI4 SGDID:S000002383, Chr IV from 56347-54398, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division; Whi4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] WHI4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Protein WHI4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=WHI4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] WHI4 SGDID:S000002383, Chr IV from 56346-54397, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" [yeast-030211-contam.fasta] WHI4 SGDID:S000002383, Chr IV from 56346-54397, Genome Release 64-2-1, reverse complement, Verified ORF, "Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR434\|c0_g1_i1\|g.335 ORF TR434\|c0_g1_i1\|g.335 TR434\|c0_g1_i1\|m.335 type:complete len:650 (+) TR434\|c0_g1_i1:368-2317(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDL224C SGDID:S000002383, Chr IV from 56346-54397, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_1148\|g.113 ORF asmbl_1148\|g.113 asmbl_1148\|m.113 type:complete len:650 (+) asmbl_1148:365-2314(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_1153\|g.775 ORF ID=asmbl_1153\|g.775 ID=asmbl_1153\|m.775 type:complete len:650 (+) ID=asmbl_1153:365-2314(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	649 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	regulation of cell size [IGI]
Molecular Function:	RNA binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLVHNQTNL NESKFLIERA FSSSSETVPL SKEATYPMPT AYSFSAVRSN SETNIKRENP  60
   61 QGFAKEPIMT SMLHNLTMST GKGNGNDVNS LAPHDVDVGP YCLLLRNLPK DITLRECYCI 120
  121 FSLATGVSSI ELKRDDREPF NDNEKVVVVK FGSLSLVTHY ANILNSKSEI FGPSFPFRSH 180
  181 IDVVNEQTQL PVSFQEHVSS GTTNSSPKNY QLSSSAQNEI QNQSFNTISY GKTSSSPLGP 240
  241 SAAKPRPSLL SERSLRFSFN DPFGLETISQ RKESVPFLRN SISQHDLSNV TTTPVPAGMP 300
  301 PQKDAGKSLL LLEKDEINES IWNGDELVND VGNSSFGASL QEPPMSSTPV MEWNASSTAN 360
  361 IPLFQLSSQE NHQSNLLPPS HHSISQDVPH IQSQPNLNNS GVIHSATSLP HYHLLNQINA 420
  421 STKTQSIQQS VSNVPSNLDL NLQTENGHPQ SSAPNGSSIF NNQKVNQGFL VSEQDTSTIS 480
  481 RQKECSSTAS ASAFSKNNET NVAGSTTISQ ADLSLLAKVP PPANPADQNP PCNTLYVGNL 540
  541 PPDATEQELR QLFSNQQGFR RLSFRNKMNS HGHGNGHGHG PICFVEFEDV SFATRALAEL 600
  601 YGSQLPHPRP SLNNKGGIRL SFSKNPLGVR GSNSRSKSGY SFNGSYGKS

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CAB3	WHI4	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	WHI4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..326]	deduced	N/A	No confident structure predictions are available.
2	View Details	[327..447]	MSA	4.225997	View MSA. No confident structure predictions are available.
3	View Details	[448..511]	deduced	N/A	No confident structure predictions are available.
4	View Details	[512..649]	PSI-BLAST	37.232149	Splicesomal U1A protein

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.80563734517858	bayes_pls_golite062009
nucleic acid binding	2.10800250002791	bayes_pls_golite062009
RNA binding	1.27892507616795	bayes_pls_golite062009
protein binding	1.02232785292059	bayes_pls_golite062009
mRNA binding	0.358102192108578	bayes_pls_golite062009

Term	Confidence	Notes
binding	2.80563734517858	bayes_pls_golite062009
nucleic acid binding	2.10800250002791	bayes_pls_golite062009
RNA binding	1.27892507616795	bayes_pls_golite062009
protein binding	1.02232785292059	bayes_pls_golite062009
mRNA binding	0.358102192108578	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2