Protein Overview: PHO2

General Information:
Name(s) found:	PHO2 / YDL106C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|A25872 transcription regulator GRF10 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320097\|ref\|NP_010177.1\| Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; Pho2p [Saccharo [NCBI NR] gi\|4144\|emb\|CAA28729.1\| PHO2 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|172152\|gb\|AAA34866.1\| PHO2 protein [NCBI NR] gi\|1431150\|emb\|CAA98673.1\| GRF10 [Saccharomyces cerevisiae] [NCBI NR] gi\|130116\|sp\|P07269.1\|PHO2_YEAST RecName: Full=Regulatory protein PHO2; AltName: Full=General regulatory factor 10 [NCBI NR] gi\|1199542\|emb\|CAA64906.1\| PHO2 [Saccharomyces cerevisiae] [NCBI NR] Regulatory protein PHO2 OS=Saccharomyces cerevisiae GN=PHO2 PE=1 SV=1 [Swiss-Prot] PHO2 Homeodomain protein [mips] Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p [SGD] PHO2, Chr IV from 270221-271900, reverse complement [yeast_orfs2] Chr IV from 270221-271900, reverse complement [yeastorf3.fasta] Chr IV from 271900-270221, reverse complement [yeastorf5.fasta] (P07269) Regulatory protein PHO2 (General regulatory factor 10) [4932.FASTACCERV.txt] PHO2 SGDID:S0002264, Chr IV from 271901-270222, reverse complement, Verified ORF [yeastflysequest.fasta] Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p [Saccharomyces cerevisiae]gi\|1199542\|emb\|CA [nrdb_11152004_Chlamydomonas] PHO2 SGDID:S000002264, Chr IV from 271901-270222, reverse complement, Verified ORF, "Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PHO2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GRF10 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PHO2 protein [nr-271106-contam.fasta] Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; Pho2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Regulatory protein PHO2 (General regulatory factor 10) [nr-271106-contam.fasta] PHO2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PHO2 SGDID:S000002264, Chr IV from 271901-270222, reverse complement, Verified ORF, "Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Regulatory protein PHO2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PHO2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PHO2 SGDID:S000002264, Chr IV from 271901-270222, Genome Release 64-1-1, reverse complement, Verified ORF, "Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p" [yeast-030211-contam.fasta] PHO2 SGDID:S000002264, Chr IV from 271901-270222, Genome Release 64-2-1, reverse complement, Verified ORF, "Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] YDL106C SGDID:S000002264, Chr IV from 271901-270222, Genome Release 64-1-1, reverse complement, Verified ORF, "Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	559 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	transcription [IGI] histidine biosynthetic process [TAS] purine base biosynthetic process [IDA] cellular response to phosphate starvation [TAS]
Molecular Function:	transcription factor activity [IGI][IMP][IPI][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMEEFSYDHD FNTHFATDLD YLQHDQQQQQ QQQHDQQHNQ QQQPQPQPIQ TQNLEHDHDQ  60
   61 HTNDMSASSN ASDSGPQRPK RTRAKGEALD VLKRKFEINP TPSLVERKKI SDLIGMPEKN 120
  121 VRIWFQNRRA KLRKKQHGSN KDTIPSSQSR DIANDYDRGS TDNNLVTTTS TSSIFHDEDL 180
  181 TFFDRIPLNS NNNYYFFDIC SITVGSWNRM KSGALQRRNF QSIKELRNLS PIKINNIMSN 240
  241 ATDLMVLISK KNSEINYFFS AMANNTKILF RIFFPLSSVT NCSLTLETDD DIINSNNTSD 300
  301 KNNSNTNNDD DNDDNSNEDN DNSSEDKRNA KDNFGELKLT VTRSPTFAVY FLNNAPDEDP 360
  361 NLNNQWSICD DFSEGRQVND AFVGGSNIPH TLKGLQKSLR FMNSLILDYK SSNEILPTIN 420
  421 TAIPTAAVPQ QNIAPPFLNT NSSATDSNPN TNLEDSLFFD HDLLSSSITN TNNGQGSNNG 480
  481 RQASKDDTLN LLDTTVNSNN NHNANNEENH LAQEHLSNDA DIVANPNDHL LSLPTDSELP 540
  541 NTPDFLKNTN ELTDEHRWI

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HRT1	PHO2	2	Unpublished Fields Lab Data
View Screen	CDC23	PHO2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PHO2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..138]	PSI-BLAST	83.529817	Paired protein
2	View Details	[139..295] [329..340]	ORFEUS	7.23	Adenovirus hexon
3	View Details	[296..328] [341..559]	ORFEUS	7.23	Adenovirus hexon
4	View Details	[458..559]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	5.13863038737912	bayes_pls_golite062009
DNA binding	4.41876985649951	bayes_pls_golite062009
nucleic acid binding	4.09724849396574	bayes_pls_golite062009
transcription factor activity	3.86239731611043	bayes_pls_golite062009
binding	3.55096238224843	bayes_pls_golite062009
sequence-specific DNA binding	2.32406445003946	bayes_pls_golite062009
RNA polymerase II transcription factor activity	2.26183812259792	bayes_pls_golite062009
transcription repressor activity	1.91962622287073	bayes_pls_golite062009
transcription activator activity	1.77700860354597	bayes_pls_golite062009
transcription factor binding	1.40653841901929	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	1.04108499638264	bayes_pls_golite062009
protein binding	0.891078200474841	bayes_pls_golite062009
transcription cofactor activity	0.84983974758882	bayes_pls_golite062009
chromatin binding	0.821515245941799	bayes_pls_golite062009
transcription corepressor activity	0.12996981272574	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2