Protein Overview: MET8

General Information:
Name(s) found:	MET8 / YBR213W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319690\|ref\|NP_009772.1\| Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis; Met8p [Saccharomyces cerevisi [NCBI NR] gi\|536598\|emb\|CAA85177.1\| MET8 [Saccharomyces cerevisiae] [NCBI NR] gi\|3934\|emb\|CAA35173.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|127028\|sp\|P15807.1\|MET8_YEAST RecName: Full=Siroheme biosynthesis protein MET8; Includes: RecName: Full=Precorrin-2 dehydrogenase; Includes: RecName: Full=Sirohydrochlorin ferrochelatase [NCBI NR] pir\|\|S20155 MET8 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] Siroheme biosynthesis protein MET8 OS=Saccharomyces cerevisiae GN=MET8 PE=1 SV=1 [Swiss-Prot] MET8 Siroheme synthase [mips] Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis [SGD] MET8, Chr II from 650326-651150 [yeast_orfs2] Chr II from 650326-651150 [yeastorf3.fasta] Chr II from 650325-651149 [yeastorf4.fasta] Chr II from 650323-651147 [yeastorf5.fasta] (P15807) Siroheme biosynthesis protein MET8 [Includes: Precorrin-2 oxidase (EC 1.-.-.-); Ferrochelatase (EC 4.99.1.-)] [4932.FASTACCERV.txt] MET8 SGDID:S0000417, Chr II from 650325-651149, Verified ORF [yeastflysequest.fasta] MET8 SGDID:S0000417, Chr II from 650363-651187, Verified ORF [10112005cervSGD.fasta] Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme; also involved in the expression of PAPS reductase and sulfite reductase [Saccharomyces cerevisiae]gi\|3934\|emb\|CAA35173.1\| unnamed protein product [Saccharomyces cere [nrdb_11152004_Chlamydomonas] (P15807) Siroheme biosynthesis protein MET8 [Includes: Precorrin-2 dehydrogenase (EC 1.3.1.76); Sirohydrochlorin ferrochelatase (EC 4.99.1.4)] [cerv4932.FASTAC] MET8 SGDID:S000000417, Chr II from 650363-651187, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme; also involved in the expression of PAPS reductase and sulfite reductase" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MET8 SGDID:S000000417, Chr II from 650363-651187, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosyn [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme; also involved in the expression of PAPS reductase and sulfite reductase; Met8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Siroheme biosynthesis protein MET8 [Includes: Precorrin-2 dehydrogenase ; Sirohydrochlorin ferrochelatase ] [nr-271106-contam.fasta] MET8 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MET8 SGDID:S000000417, Chr II from 650363-651187, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Siroheme biosynthesis protein MET8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MET8 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MET8 SGDID:S000000417, Chr II from 650368-651192, Genome Release 64-1-1, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis" [yeast-030211-contam.fasta] MET8 SGDID:S000000417, Chr II from 650368-651192, Genome Release 64-2-1, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis" [20141208_orf_trans.fasta] YBR213W SGDID:S000000417, Chr II from 650368-651192, Genome Release 64-1-1, Verified ORF, "Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	274 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	response to drug [IMP] siroheme biosynthetic process [IMP] sulfate assimilation [IMP]
Molecular Function:	ferrochelatase activity [IDA] precorrin-2 dehydrogenase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKSLQLAHQ LKDKKILLIG GGEVGLTRLY KLIPTGCKLT LVSPDLHKSI IPKFGKFIQN  60
   61 EDQPDYREDA KRFINPNWDP TKNEIYEYIR SDFKDEYLDL EDENDAWYII MTCIPDHPES 120
  121 ARIYHLCKER FGKQQLVNVA DKPDLCDFYF GANLEIGDRL QILISTNGLS PRFGALVRDE 180
  181 IRNLFTQMGD LALEDAVVKL GELRRGIRLL APDDKDVKYR MDWARRCTDL FGIQHCHNID 240
  241 VKRLLDLFKV MFQEQNCSLQ FPPRERLLSE YCSS

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GBP2	MET8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..179]	PSI-BLAST	1608.221849	Bifunctional dehydrogenase/ferrochelatase Met8p, N-terminal domain; Bifunctional dehydrogenase/ferrochelatase Met8p, dimerisation and C-terminal domains
2	View Details	[180..274]	PSI-BLAST	1608.221849	Bifunctional dehydrogenase/ferrochelatase Met8p, N-terminal domain; Bifunctional dehydrogenase/ferrochelatase Met8p, dimerisation and C-terminal domains

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	1.95832530533882	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
DNA binding	1.70073234884672	bayes_pls_golite062009
binding	1.50616426395139	bayes_pls_golite062009
nucleic acid binding	1.47211855405194	bayes_pls_golite062009
transcription factor activity	0.9895904275677	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2