Protein Overview: CCZ1

General Information:
Name(s) found:	CCZ1 / YBR131W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319607\|ref\|NP_009689.1\| Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway; Ccz1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S46000 probable membrane protein YBR131w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|586541\|sp\|P38273.1\|CCZ1_YEAST RecName: Full=Vacuolar fusion protein CCZ1; AltName: Full=Calcium-caffeine-zinc sensitivity protein 1; AltName: Full=Autophagy-related protein 11; AltName: Full=Cytoplasm to vacuole targeting protein 16 [NCBI NR] gi\|536421\|emb\|CAA85088.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|51013867\|gb\|AAT93227.1\| YBR131W [Saccharomyces cerevisiae] [NCBI NR] gi\|496862\|emb\|CAA53490.1\| YBR1006 [Saccharomyces cerevisiae] [NCBI NR] gi\|1582518\|prf\|\|2118402F YBR1006 gene [NCBI NR] (P38273) Uncharacterized protein YBR131W [Swiss-Prot] Vacuolar fusion protein CCZ1 OS=Saccharomyces cerevisiae GN=CCZ1 PE=1 SV=1 [Swiss-Prot] CCZ1 Calcium Caffeine Zinc sensitivity [mips] Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway [SGD] CCZ1, Chr II from 497121-499235 [yeast_orfs2] Chr II from 497121-499235 [yeastorf3.fasta] Chr II from 497120-499234 [yeastorf4.fasta] Chr II from 497118-499232 [yeastorf5.fasta] (P38273) Hypothetical 80.7 kDa protein in OPY1-AGP2 intergenic region [4932.FASTACCERV.txt] CCZ1 SGDID:S0000335, Chr II from 497119-499233, Verified ORF [yeastflysequest.fasta] CCZ1 SGDID:S0000335, Chr II from 497157-499271, Verified ORF [10112005cervSGD.fasta] Calcium Caffeine Zinc sensitivity; involved in sporulation, caffeine, calcium, and zinc sensitivity [Saccharomyces cerevisiae]gi\|51013867\|gb\|AAT93227.1\| YBR131W [Saccharomyces cerevisiae]gi\|496862\|emb\|CAA53490.1\| YBR1006 [Saccharomyces cerevisiae]gi\|53 [nrdb_11152004_Chlamydomonas] CCZ1 SGDID:S000000335, Chr II from 497157-499271, Verified ORF, "Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YBR1006 gene [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized protein YBR131W [nr-271106-contam.fasta] YBR131W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway; Ccz1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YBR1006 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Vacuolar fusion protein CCZ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CCZ1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CCZ1 SGDID:S000000335, Chr II from 497163-499277, Genome Release 64-1-1, Verified ORF, "Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway" [yeast-030211-contam.fasta] CCZ1 SGDID:S000000335, Chr II from 497163-499277, Genome Release 64-2-1, Verified ORF, "Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy" [20141208_orf_trans.fasta] YBR131W SGDID:S000000335, Chr II from 497163-499277, Genome Release 64-1-1, Verified ORF, "Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	704 amino acids
Gene Ontology:
Cellular Component:	membrane [TAS] late endosome [TAS] vacuole [IDA]
Biological Process:	autophagy [IDA] vesicle docking [IMP] vacuolar transport [IGI][IMP] protein targeting to vacuole [IMP] autophagic vacuole fusion [IDA]
Molecular Function:	guanyl-nucleotide exchange factor activity [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRLHYITVFD PSRSTNENDT FKQLLLFHYF GTTDSIPSLN EKLSIIGVIQ GIWSLTSSCV  60
   61 NKDGEDLEKI IELNNDIIFC IKVESRFFIS LAISNISDDQ SAIPLQYLSA YLWLSYRFFK 120
  121 LLNGSFSGFN KDFRKLTDLL NEFVIPFWND IYLNLETVTN RSFTVMWPGF YKRANFQHSS 180
  181 YNPGEKNNVE ESWDAIILQN ILLDKKSYLG LKDILVYHLP KRTKAANRES MGTKTYGLVR 240
  241 NFTSDLNTLP DISNWLYHLH CTYGEISSHI LTGNVHFKEE LQVEEEQERS RDTNGRDEEE 300
  301 SQEQQRREHQ ETTQNNTSEL SLSERVIHNV TLPISFAYDA IHEVSTTTGV SGSLSMIMDY 360
  361 VPKPHWPFIS SSNKSADKNN YSNSNDNANS NAPLMAQSEA VGGTIGNSRF GFLISPLNSD 420
  421 LLPSSYQALK LNLNFENSKD KEDFYNCLFW YFDDFLIVIV CDPDFNKICE RDYLKDLSFQ 480
  481 LCQSMECLNN EILNSQNCDN VESFAYVIRD NVTKEIDSSV PFGSPKFTSD ESISTLQLAI 540
  541 NGIDQFINDN SNSLSLANWN PITIMGGSNA ISKKNTTEGF GNGVNDKTQK FKRKYLNFLN 600
  601 LMSAEKLWDL QVDVLQFLTS LQNSKRDPDY FQEERLLKLN NGVLCYIKEN NSNLIIIIKN 660
  661 WFQNNGTSKA AKQRNRFSSD SSKGSSLFQS LGRDVTDWWE SREI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		CCZ1 MON1 YAP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAS2	CCZ1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CCZ1 and SNF7	Huh WK, et al. (2003)
View Data	CCZ1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..704]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2