YRC Public Data Repository - Protein Structure Prediction

Protein:	pus1
Organism:	Schizosaccharomyces pombe
Length:	534 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for pus1.

Description	E-value	Query Range	Subject Range
gi\|32414669, gi\|... - gi\|32414669\|ref\|XP_327814.1\| hypothetical protein [Neurospora crassa], gi\|28920438\|gb\|EAA29805.1\| hy... gi\|85091723 - gi\|85091723\|ref\|XP_959041.1\| hypothetical protein NCU07528 [Neurospora crassa OR74A]	393.0	[0..6]	[528..83]
gi\|42553476, gi\|... - gi\|46124417\|ref\|XP_386762.1\| hypothetical protein FG06586.1 [Gibberella zeae PH-1], gi\|42553476\|gb\|E...	374.0	[0..5]	[515..60]
PUS1_ASHGO - tRNA pseudouridine synthase 1 OS=Ashbya gossypii GN=PUS1 PE=3 SV=1 PUS1_ASHGO - tRNA pseudouridine synthase 1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL ...	370.0	[0..6]	[517..31]
gi\|88184224, gi\|... - gi\|88184224\|gb\|EAQ91692.1\| hypothetical protein CHGG_03627 [Chaetomium globosum CBS 148.51], gi\|1162...	369.0	[0..2]	[526..66]
gi\|40738761, gi\|... - gi\|67539990\|ref\|XP_663769.1\| hypothetical protein AN6165.2 [Aspergillus nidulans FGSC A4], ref\|XP_41...	355.0	[0..2]	[533..37]
gi\|90305863, gi\|... - gi\|90305863\|gb\|EAS35494.1\| tRNA pseudouridine synthase 1, putative [Coccidioides immitis RS], gi\|119...	346.0	[0..6]	[502..72]
gi\|115442996, gi... - gi\|115442996\|ref\|XP_001218305.1\| conserved hypothetical protein [Aspergillus terreus NIH2624], gi\|11...	344.0	[0..3]	[529..30]
PUS1_YEAST - tRNA pseudouridine synthase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS1 PE=1 ... gi\|151942589 - gi\|151942589\|gb\|EDN60935.1\| tRNA-pseudouridine synthase [Saccharomyces cerevisiae YJM789] PUS1 - tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA... gi\|190407751 - gi\|190407751\|gb\|EDV11016.1\| tRNA pseudouridine synthase [Saccharomyces cerevisiae RM11-1a]	341.0	[0..21]	[517..52]
PUS1_CANGA - tRNA pseudouridine synthase 1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622... PUS1_CANGA - tRNA pseudouridine synthase 1 OS=Candida glabrata GN=PUS1 PE=3 SV=1	336.0	[0..7]	[530..36]
gi\|71000998, gi\|... - gi\|71000998\|ref\|XP_755180.1\| tRNA pseudouridine synthase [Aspergillus fumigatus Af293], gi\|66852818\|... gi\|159129271 - gi\|159129271\|gb\|EDP54385.1\| tRNA pseudouridine synthase, putative [Aspergillus fumigatus A1163]	332.0	[0..3]	[529..38]

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Predicted Domain #1

Region A:
Residues: [1-289]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGRGGKRTWY NGDRREAKRN RPNSIYNGEG RPENLVVGEK KPKRKVACLV GYCGSGYHGM  60
   61 QLNPPSKTIE GDLFDAFVKA GAVSSYNADD PKKVALARAA RTDKGVHAAG NVISLKLIME 120
  121 DEKLIEKVNE HLPPSIRLWD VIRTINSFNP RTYCESRIYE YMVPTYAFVP PKPSSILGNC 180
  181 IMKNSPMPAE PINKENINQL SRSLFYEEGK EFWDDYDIAA KEILSLYEQD PEGFVNPYSK 240
  241 RGAAALANSE NNKGSEAGVS AKTNPDMDSD SSAIVNEFLK PDSVEDESA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	72.0
Match:	2nqpA
Description:	No description for 2nqpA was found.

Predicted functions:

Term	Confidence	Notes
pseudouridine synthase activity	5.19957135839682	bayes_pls_golite062009
intramolecular transferase activity	3.78688422405974	bayes_pls_golite062009
isomerase activity	2.91720316078381	bayes_pls_golite062009
catalytic activity	2.02180970970004	bayes_pls_golite062009
transcription regulator activity	1.21173188039059	bayes_pls_golite062009
DNA binding	0.950713703876672	bayes_pls_golite062009
nucleic acid binding	0.909704702595901	bayes_pls_golite062009
binding	0.730744558777463	bayes_pls_golite062009
RNA binding	0.706879359688019	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [290-438]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GSKIDPSYRL ERALKHIEVL KLKNYRISAD RLSVIRETLN QYVGVHNFHN FTVGQAFHQK  60
   61 NSNRVIRSFT ASDPFMIGDT EWISCKVHGQ SFMLHQIRKM IALAILVVRT GCPVERIQDA 120
  121 FKKTKINIPK GPGFGLLLES PFFKGYNEH

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	4.92
Match:	1vs3A
Description:	No description for 1vs3A was found.

Predicted Domain #3

Region A:
Residues: [439-534]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KAPENNRDPI DFTKYEQKIT AFKHAHIYDK IFLEEARKQV FHCFLSFIDS YNEEDFSYLS  60
   61 DIGITEKTQE VSSKLPDVLS SDEEEDSAEN KDDLEG

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

		MCM Score	GO Score	GO Term	SCOP Match	SCOP Description
View	Download	0.855	0.046	pseudouridylate synthase activity	a.60.6	DNA polymerase beta, N-terminal domain-like

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: