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View Structure Prediction Details

Protein: TfIIFbeta-PA
Organism: Drosophila melanogaster
Length: 277 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TfIIFbeta-PA.

Description E-value Query
Range
Subject
Range
gi|450771 - gi|450771|gb|AAA81888.1| dTFIIF30
367.0 [0..1] [277..1]
gi|54639748|gb|E... - GA19669-PA [Drosophila pseudoobscura], GA19669 [Drosophila pseudoobscura pseudoobscura]
gi|54639748, gi|... - gi|54639748|gb|EAL29150.1| GA19669 [Drosophila pseudoobscura pseudoobscura], gi|125778480|ref|XP_001...
363.0 [0..1] [277..1]
gi|55245850, gi|... - gi|58376648|ref|XP_308768.2| ENSANGP00000015059 [Anopheles gambiae str. PEST], gi|55245850|gb|EAA040...
327.0 [0..1] [261..1]
gi|108869504, gi... - gi|157138343|ref|XP_001657254.1| hypothetical protein AaeL_AAEL013998 [Aedes aegypti], gi|108869504|...
325.0 [0..6] [277..4]
gi|50730835 - gi|50730835|ref|XP_417039.1| PREDICTED: similar to General transcription factor IIF, polypeptide 2 [...
317.0 [0..12] [266..81]
gi|73989304 - gi|73989304|ref|XP_851466.1| PREDICTED: similar to general transcription factor IIF, polypeptide 2 [...
317.0 [0..14] [266..112]
gi|38174399, gi|... - gi|45360629|ref|NP_988987.1| general transcription factor 2F, polypeptide 2, 30kDa [Xenopus (Siluran...
311.0 [0..12] [266..4]
T2FB_BOVIN - General transcription factor IIF subunit 2 OS=Bos taurus GN=GTF2F2 PE=2 SV=1
307.0 [0..14] [266..5]
gi|62901918 - gi|62901918|gb|AAY18910.1| RAP30 [synthetic construct]
307.0 [0..14] [266..29]

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Predicted Domain #1
Region A:
Residues: [1-137]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKEDKEKTQ IIDKDLDLSN AGRGVWLVKV PKYIAQKWEK APTNMDVGKL RINKTPGQKA  60
   61 QVSLSLTPAV LALDPEEKIP TEHILDVSQV TKQTLGVFSH MAPSDGKENS TTSAAQPDNE 120
  121 KLYMEGRIVQ KLECRPI

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 31.045757
Match: 1f3uA
Description: TFIIF beta subunit, Rap30
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
general RNA polymerase II transcription factor activity 10.2797850053222 bayes_pls_golite062009
transcription initiation factor activity 6.22439849848084 bayes_pls_golite062009
RNA polymerase II transcription factor activity 4.06672086326535 bayes_pls_golite062009
transcription regulator activity 3.89911747857156 bayes_pls_golite062009
binding 2.17316238057078 bayes_pls_golite062009
transcription activator activity 2.12958061949296 bayes_pls_golite062009
protein binding 1.90482509358938 bayes_pls_golite062009
transcription coactivator activity 1.30173573105398 bayes_pls_golite062009
transcription factor binding 0.677361513203971 bayes_pls_golite062009
transcription cofactor activity 0.43974291826617 bayes_pls_golite062009
catalytic activity 0.304516551414824 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [138-277]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ADNCYMKLKL ESIRKASEPQ RRVQPIDKIV QNFKPVKDHA HNIEYRERKK AEGKKARDDK  60
   61 NAVMDMLFHA FEKHQYYNIK DLVKITNQPI SYLKEILKDV CDYNMKNPHK NMWELKKEYR 120
  121 HYKTEEKKEE EHKSGSSDSE 

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 26.69897
Match: 1bbyA
Description: DNA-BINDING DOMAIN FROM HUMAN RAP30, NMR, MINIMIZED AVERAGE
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
general RNA polymerase II transcription factor activity 5.67074370996647 bayes_pls_golite062009
transcription regulator activity 5.55329976718261 bayes_pls_golite062009
DNA binding 5.08959163553491 bayes_pls_golite062009
nucleic acid binding 4.99681957904233 bayes_pls_golite062009
transcription factor activity 4.33152142980289 bayes_pls_golite062009
transcription activator activity 3.70589483230587 bayes_pls_golite062009
sequence-specific DNA binding 3.58179858678904 bayes_pls_golite062009
binding 3.52874320222492 bayes_pls_golite062009
RNA polymerase II transcription factor activity 2.60738445921725 bayes_pls_golite062009
transcription repressor activity 2.22485867684613 bayes_pls_golite062009
DNA-directed RNA polymerase activity 1.82365519759474 bayes_pls_golite062009
RNA polymerase activity 1.82365519759474 bayes_pls_golite062009
transcription elongation regulator activity 1.81270943531115 bayes_pls_golite062009
protein binding 1.80415286835431 bayes_pls_golite062009
RNA binding 0.992043085614693 bayes_pls_golite062009
transcription factor binding 0.85714789329232 bayes_pls_golite062009
specific RNA polymerase II transcription factor activity 0.66743181561551 bayes_pls_golite062009
nucleotidyltransferase activity 0.237727660028553 bayes_pls_golite062009
RNA polymerase II transcription factor activity, enhancer binding 0.23177057841536 bayes_pls_golite062009
positive transcription elongation factor activity 0.13524243942225 bayes_pls_golite062009
structural molecule activity 0.111941063082739 bayes_pls_golite062009

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