YRC Public Data Repository - Protein Structure Prediction

Protein:	GEF1
Organism:	Saccharomyces cerevisiae
Length:	779 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GEF1.

Description	E-value	Query Range	Subject Range
GEF1_YEAST - Anion/proton exchange transporter GEF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=G... GEF1 - Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, an...	0.0	[1..779]	[1..779]
CLCN3_RABIT - H(+)/Cl(-) exchange transporter 3 OS=Oryctolagus cuniculus GN=CLCN3 PE=2 SV=3 CLCN3 - chloride channel 3 gi\|114596820, gi... - gi\|114596820\|ref\|XP_001153970.1\| PREDICTED: chloride channel 3 isoform 5 [Pan troglodytes], gi\|11459...	0.0	[4..741]	[51..808]
CLCN3_MOUSE - H(+)/Cl(-) exchange transporter 3 OS=Mus musculus GN=Clcn3 PE=1 SV=2	0.0	[4..741]	[51..808]
CLCN3_RAT - H(+)/Cl(-) exchange transporter 3 OS=Rattus norvegicus GN=Clcn3 PE=2 SV=2	0.0	[4..741]	[51..808]
gi\|5923861 - gi\|5923861\|gb\|AAD56388.1\|AF182215_1 chloride channel CLC-3 [Oreochromis mossambicus]	0.0	[13..739]	[4..747]
CLCN3_CAVPO - H(+)/Cl(-) exchange transporter 3 OS=Cavia porcellus GN=CLCN3 PE=2 SV=1	0.0	[13..741]	[3..750]

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Predicted Domain #1

Region A:
Residues: [1-97]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPTTYVPINQ PIGDGEDVID TNRFTNIPET QNFDQFVTID KIAEENRPLS VDSDREFLNS  60
   61 KYRHYREVIW DRAKTFITLS STAIVIGCIA GFLQVFT

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [189-209]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SEIKVWVSGF EYNKEFLGFL T

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [232-325]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PSVHYATCCG YLLTKWLLRD TLTYSSQYEY ITAASGAGVA VAFGAPIGGV LFGLEEIASA  60
   61 NRFNSSTLWK SYYVALVAIT TLKYIDPFRN GRVI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	224.221849
Match:	1kplA_
Description:	Clc chloride channel
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [98-188]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ETLVNWKTGH CQRNWLLNKS FCCNGVVNEV TSTSNLLLKR QEFECEAQGL WIAWKGHVSP  60
   61 FIIFMLLSVL FALISTLLVK YVAPMATGSG I

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [210-231]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LVIKSVALPL AISSGLSVGK EG

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [326-571]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LFNVTYDRDW KVQEIPIFIA LGIFGGLYGK YISKWNINFI HFRKMYLSSW PVQEVLFLAT  60
   61 LTALISYFNE FLKLDMTESM GILFHECVKN DNTSTFSHRL CQLDENTHAF EFLKIFTSLC 120
  121 FATVIRALLV VVSYGARVPA GIFVPSMAVG ATFGRAVSLL VERFISGPSV ITPGAYAFLG 180
  181 AAATLSGITN LTLTVVVIMF ELTGAFMYII PLMIVVAITR IILSTSGISG GIADQMIMVN 240
  241 GFPYLE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	224.221849
Match:	1kplA_
Description:	Clc chloride channel
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
anion channel activity	6.77290758049983	bayes_pls_golite062009
chloride channel activity	6.77290758049983	bayes_pls_golite062009
anion transmembrane transporter activity	6.08111514985329	bayes_pls_golite062009
voltage-gated chloride channel activity	5.99544422864122	bayes_pls_golite062009
voltage-gated anion channel activity	5.99544422864122	bayes_pls_golite062009
ion transmembrane transporter activity	5.07840957725271	bayes_pls_golite062009
ion channel activity	4.93164650815682	bayes_pls_golite062009
substrate-specific channel activity	4.81149777353187	bayes_pls_golite062009
channel activity	4.78965126968592	bayes_pls_golite062009
passive transmembrane transporter activity	4.78965126968592	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	4.67227438984104	bayes_pls_golite062009
voltage-gated ion channel activity	4.27956097534048	bayes_pls_golite062009
voltage-gated channel activity	4.255638017629	bayes_pls_golite062009
gated channel activity	4.1072949331811	bayes_pls_golite062009
transporter activity	2.94995161862691	bayes_pls_golite062009
transmembrane transporter activity	2.84443650880263	bayes_pls_golite062009
substrate-specific transporter activity	2.82470397063503	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [572-779]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DEQDEEEEET LEKYTAEQLM SSKLITINET IYLSELESLL YDSASEYSVH GFPITKDEDK  60
   61 FEKEKRCIGY VLKRHLASKI MMQSVNSTKA QTTLVYFNKS NEELGHRENC IGFKDIMNES 120
  121 PISVKKAVPV TLLFRMFKEL GCKTIIVEES GILKGLVTAK DILRFKRIKY REVHGAKFTY 180
  181 NEALDRRCWS VIHFIIKRFT TNRNGNVI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	27.154902
Match:	1b3oA_
Description:	Inosine monophosphate dehydrogenase (IMPDH)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: