YRC Public Data Repository - Protein Structure Prediction

Protein:	KAR2
Organism:	Saccharomyces cerevisiae
Length:	682 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for KAR2.

Description	E-value	Query Range	Subject Range
HSP70_TRIRU - Heat shock 70 kDa protein OS=Trichophyton rubrum GN=HSP70 PE=2 SV=1	0.0	[49..654]	[1..610]
gi\|6969976 - gi\|6969976\|gb\|AAF34134.1\| high molecular weight heat shock protein [Malus x domestica]	0.0	[47..680]	[4..650]
gi\|2894792, gi\|1... - gi\|2894792\|gb\|AAC02807.1\| heat shock protein 70 [Cryptosporidium parvum], gi\|1616783\|gb\|AAB16853.1\| ...	0.0	[46..657]	[2..618]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[46..680]	[2..641]
HSP70_TRYCR - Heat shock 70 kDa protein OS=Trypanosoma cruzi GN=HSP70 PE=3 SV=1	0.0	[49..655]	[3..615]
HSP70_LEIDO - Heat shock 70 kDa protein OS=Leishmania donovani GN=HSP70 PE=3 SV=1	0.0	[52..656]	[6..615]
HSP7C_ICTPU - Heat shock cognate 71 kDa protein OS=Ictalurus punctatus GN=hsc70 PE=2 SV=1	0.0	[48..656]	[2..613]
gi\|1620381 - gi\|1620381\|emb\|CAA69891.1\| 70 kD heat shock protein [Takifugu rubripes]	0.0	[46..567]	[2..526]

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Predicted Domain #1

Region A:
Residues: [1-220]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG TVIGIDLGTT  60
   61 YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA AKNQVAANPQ NTIFDIKRLI 120
  121 GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE VSVKGEKKVF TPEEISGMIL GKMKQIAEDY 180
  181 LGTKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVLRIVN

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [412-428]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EAVAYGAAVQ AGVLSGE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2450.0
Match:	1hpm__
Description:	Heat shock protein 70kDa, ATPase fragment
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
unfolded protein binding	7.97191512372019	bayes_pls_golite062009
structural molecule activity	3.59589495899762	bayes_pls_golite062009
structural constituent of cytoskeleton	2.46121037473545	bayes_pls_golite062009
carbohydrate kinase activity	2.33338276303563	bayes_pls_golite062009
cytoskeletal protein binding	2.20983242331685	bayes_pls_golite062009
binding	2.11855816699924	bayes_pls_golite062009
protein binding	2.00711904074672	bayes_pls_golite062009
hydrolase activity	1.88613511243568	bayes_pls_golite062009
hexokinase activity	1.70516840340504	bayes_pls_golite062009
transcription regulator activity	1.68670098354938	bayes_pls_golite062009
nucleic acid binding	1.43834016708999	bayes_pls_golite062009
peptidase activity	1.4127499531612	bayes_pls_golite062009
DNA binding	1.36804228074586	bayes_pls_golite062009
transporter activity	1.2883466875237	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.16749728168925	bayes_pls_golite062009
kinase activity	1.11102445353265	bayes_pls_golite062009
actin binding	1.03829083423378	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.953663805249074	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.900931843471417	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.898645342556399	bayes_pls_golite062009
pyrophosphatase activity	0.875671218597808	bayes_pls_golite062009
substrate-specific transporter activity	0.796517590178929	bayes_pls_golite062009
transcription factor activity	0.783439770973386	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.78242344952806	bayes_pls_golite062009
protein transporter activity	0.780308299678282	bayes_pls_golite062009
ATPase activity	0.687771333492666	bayes_pls_golite062009
myosin binding	0.607451856096	bayes_pls_golite062009
glucose binding	0.53527958884988	bayes_pls_golite062009
glucokinase activity	0.47468456642723	bayes_pls_golite062009
catalytic activity	0.296484721581333	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.186218331473028	bayes_pls_golite062009
chromatin binding	0.155140608043269	bayes_pls_golite062009
protease binding	0.121259015422625	bayes_pls_golite062009
chaperone binding	0.00111313452804307	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [221-276]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EPTAAAIAYG LDKSDKEHQI IVYDLGGGTF DVSLLSIENG VFEVQATSGD THLGGE

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [352-411]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LDLFKKTLKP VEKVLQDSGL EKKDVDDIVL VGGSTRIPKV QQLLESYFDG KKASKGINPD  60
   61

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2450.0
Match:	1hpm__
Description:	Heat shock protein 70kDa, ATPase fragment
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [277-351]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DFDYKIVRQL IKAFKKKHGI DVSDNNKALA KLKREAEKAK RALSSQMSTR IEIDSFVDGI  60
   61 DLSETLTRAK FEELN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2450.0
Match:	1hpm__
Description:	Heat shock protein 70kDa, ATPase fragment
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [429-586]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EGVEDIVLLD VNALTLGIET TGGVMTPLIK RNTAIPTKKS QIFSTAVDNQ PTVMIKVYEG  60
   61 ERAMSKDNNL LGKFELTGIP PAPRGVPQIE VTFALDANGI LKVSATDKGT GKSESITITN 120
  121 DKGRLTQEEI DRMVEEAEKF ASEDASIKAK VESRNKLE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	668.201249
Match:	1ckrA_
Description:	DnaK
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [587-682]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NYAHSLKNQV NGDLGEKLEE EDKETLLDAA NDVLEWLDDN FETAIAEDFD EKFESLSKVA  60
   61 YPITSKLYGG ADGSGAADYD DEDEDDDGDY FEHDEL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	3.0
Match:	1i84S_
Description:	Heavy meromyosin subfragment
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: