YRC Public Data Repository - Protein Structure Prediction

Protein:	BPL1
Organism:	Saccharomyces cerevisiae
Length:	690 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BPL1.

Description	E-value	Query Range	Subject Range
BPL1 - Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for ace... BPL1_YEAST - Biotin--protein ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BPL1 PE=1 SV=1	0.0	[1..690]	[1..690]
BPL1_SCHPO - Biotin--protein ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=bpl1 PE=3 SV=1 SPBC30D10.07c - biotin-protein ligase	0.0	[1..687]	[1..630]
gi\|1813424 - gi\|1813424\|dbj\|BAA13332.1\| HCS [Homo sapiens]	0.0	[2..689]	[163..726]
BPL1_MOUSE - Biotin--protein ligase OS=Mus musculus GN=Hlcs PE=1 SV=1	0.0	[177..689]	[290..722]
Y305_CHLMU - Uncharacterized protein TC_0305 OS=Chlamydia muridarum GN=TC_0305 PE=3 SV=1 Y305_CHLMU - Uncharacterized protein TC_0305 OS=Chlamydia muridarum (strain MoPn / Nigg) GN=TC_0305 PE=3 SV=1	0.0	[3..255]	[6..251]
gi\|231273703, gi... - gi\|237802469\|ref\|YP_002887663.1\| putative protein ligase [Chlamydia trachomatis], gi\|231273703\|emb\|C... Y035_CHLTR - Uncharacterized protein CT_035 OS=Chlamydia trachomatis (strain D/UW-3/Cx) GN=CT_035 PE=3 SV=1 Y035_CHLTR - Uncharacterized protein CT_035 OS=Chlamydia trachomatis GN=CT_035 PE=3 SV=1 gi\|76167277, gi\|... - gi\|76788747\|ref\|YP_327833.1\| biotin--protein ligase [Chlamydia trachomatis A/HAR-13], gi\|76167277\|gb...	0.0	[3..255]	[4..249]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	2.0E-96	[224..687]	[1802..2240]
gi\|7189558, gi\|1... - gi\|7189558\|gb\|AAF38458.1\| biotin apo-protein ligase-related protein [Chlamydophila pneumoniae AR39],... gi\|8978502, gi\|1... - gi\|8978502\|dbj\|BAA98339.1\| biotin protein ligase [Chlamydophila pneumoniae J138], gi\|15835664\|ref\|NP... gi\|15618052, gi\|... - gi\|4376392\|gb\|AAD18281.1\| Biotin Protein Ligase [Chlamydophila pneumoniae CWL029], gi\|15618052\|ref\|N... gi\|33241465, gi\|... - gi\|33241465\|ref\|NP_876406.1\| biotin--[acetyl-CoA-carboxylase] ligase [Chlamydophila pneumoniae TW-18... Y128_CHLPN - Uncharacterized protein CPn_0128/CP_0643/CPj0128/CpB0130 OS=Chlamydia pneumoniae GN=CPn_0128 PE=3 SV...	3.0E-94	[3..274]	[6..255]
gi\|15619878, gi\|... - gi\|15892704\|ref\|NP_360418.1\| putative N-terminal of biotin-protein ligase [Rickettsia conorii str. M... gi\|25350801 - pir\|\|E97797 hypothetical protein RC0781 [imported] - Rickettsia conorii (strain Malish 7) gi\|34580415, gi\|... - gi\|34580415\|ref\|ZP_00141895.1\| hypothetical N-terminal of biotin-protein ligase [Rickettsia sibirica...	1.0E-84	[1..258]	[1..242]
gi\|19698367 - gi\|19698367\|gb\|AAL93109.1\|AF414938_1 holocarboxylase synthetase hcs2.a [Arabidopsis thaliana]	2.0E-71	[364..688]	[57..329]

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Predicted Domain #1

Region A:
Residues: [1-263]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNVLVYNGPG TTPGSVKHAV ESLRDFLEPY YAVSTVNVKV LQTEPWMSKT SAVVFPGGAD  60
   61 LPYVQACQPI ISRLKHFVSK QGGVFIGFCA GGYFGTSRVE FAQGDPTMEV SGSRDLRFFP 120
  121 GTSRGPAYNG FQYNSEAGAR AVKLNLPDGS QFSTYFNGGA VFVDADKFDN VEILATYAEH 180
  181 PDVPSSDSGK GQSENPAAVV LCTVGRGKVL LTGPHPEFNV RFMRKSTDKH FLETVVENLK 240
  241 AQEIMRLKFM RTVLTKTGLN CNN

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [264-348]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DFNYVRAPNL TPLFMASAPN KRNYLQEMEN NLAHHGMHAN NVELCSELNA ETDSFQFYRG  60
   61 YRASYDAASS SLLHKEPDEV PKTVI

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3

Region A:
Residues: [349-622]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FPGVDEDIPP FQYTPNFDMK EYFKYLNVQN TIGSLLLYGE VVTSTSTILN NNKSLLSSIP  60
   61 ESTLLHVGTI QVSGRGRGGN TWINPKGVCA STAVVTMPLQ SPVTNRNISV VFVQYLSMLA 120
  121 YCKAILSYAP GFSDIPVRIK WPNDLYALSP TYYKRKNLKL VNTGFEHTKL PLGDIEPAYL 180
  181 KISGLLVNTH FINNKYCLLL GCGINLTSDG PTTSLQTWID ILNEERQQLH LDLLPAIKAE 240
  241 KLQALYMNNL EVILKQFINY GAAEILPSYY ELWL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	85.68867
Match:	1bia__
Description:	Biotin repressor, N-terminal domain; Biotin repressor/biotin holoenzyme synthetase, C-terminal domain; Biotin repressor/biotin holoenzyme synthetase, catalytic (central) domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
aminoacyl-tRNA ligase activity	4.83057889638134	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	4.8294067084702	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	4.8294067084702	bayes_pls_golite062009
ligase activity	3.75598318664255	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	1.35137682451633	bayes_pls_golite062009
binding	0.904147650999471	bayes_pls_golite062009
threonine-tRNA ligase activity	0.793195358470619	bayes_pls_golite062009
glycine-tRNA ligase activity	0.788242088202643	bayes_pls_golite062009
serine-tRNA ligase activity	0.681379128250628	bayes_pls_golite062009
transcription regulator activity	0.63339258210663	bayes_pls_golite062009
nucleic acid binding	0.609853081467937	bayes_pls_golite062009
DNA binding	0.48413861319521	bayes_pls_golite062009
alanine-tRNA ligase activity	0.435766663027965	bayes_pls_golite062009
histidine-tRNA ligase activity	0.334773354741163	bayes_pls_golite062009
lysine-tRNA ligase activity	0.333541891861949	bayes_pls_golite062009
ATP binding	0.18403217430648	bayes_pls_golite062009
adenyl ribonucleotide binding	0.145955341735342	bayes_pls_golite062009
adenyl nucleotide binding	0.127823774098159	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [623-690]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 HSNQIVTLPD HGNTQAMITG ITEDYGLLIA KELVSGSSTQ FTGNVYNLQP DGNTFDIFKS  60
   61 LIAKKVQS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	85.68867
Match:	1bia__
Description:	Biotin repressor, N-terminal domain; Biotin repressor/biotin holoenzyme synthetase, C-terminal domain; Biotin repressor/biotin holoenzyme synthetase, catalytic (central) domain
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: