YRC Public Data Repository - Protein Structure Prediction

Protein:	MEC1
Organism:	Saccharomyces cerevisiae
Length:	2368 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MEC1.

Description	E-value	Query Range	Subject Range
gi\|225000812 - gi\|225000812\|gb\|AAI72404.1\| Ataxia telangiectasia and Rad3 related [synthetic construct] ATR - ataxia telangiectasia and Rad3 related	0.0	[461..2367]	[710..2643]
rad3 - ATR checkpoint kinase RAD3_SCHPO - Protein kinase rad3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=rad3 PE=1 SV=2	0.0	[522..2368]	[534..2386]
ATM_MOUSE - Serine-protein kinase ATM OS=Mus musculus GN=Atm PE=1 SV=1	0.0	[698..2367]	[1226..3065]
gi\|506876 - gi\|506876\|dbj\|BAA01860.1\| Esr1 protein [Saccharomyces cerevisiae]	0.0	[1..2368]	[1..2368]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[820..2367]	[1..1707]
gi\|5853096 - gi\|5853096\|gb\|AAD54313.1\|AF176575_1 UVSB PI-3 kinase [Emericella nidulans]	0.0	[462..2368]	[520..2454]
ATR_ARATH - Serine/threonine-protein kinase ATR OS=Arabidopsis thaliana GN=ATR PE=2 SV=2	0.0	[480..2368]	[701..2702]
gi\|11385422 - gi\|11385422\|gb\|AAG34794.1\|AF223644_1 Atr protein [Xenopus laevis]	0.0	[618..2367]	[875..2653]

Back

Predicted Domain #1

Region A:
Residues: [1-555]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MESHVKYLDE LILAIKDLNS GVDSKVQIKK VPTDPSSSQE YAKSLKILNT LIRNLKDQRR  60
   61 NNIMKNDTIF SKTVSALALL LEYNPFLLVM KDSNGNFEIQ RLIDDFLNIS VLNYDNYHRI 120
  121 WFMRRKLGSW CKACVEFYGK PAKFQLTAHF ENTMNLYEQA LTEVLLGKTE LLKFYDTLKG 180
  181 LYILLYWFTS EYSTFGNSIA FLDSSLGFTK FDFNFQRLIR IVLYVFDSCE LAALEYAEIQ 240
  241 LKYISLVVDY VCNRTISTAL DAPALVCCEQ LKFVLTTMHH FLDNKYGLLD NDPTMAKGIL 300
  301 RLYSLCISND FSKCFVDHFP IDQWADFSQS EHFPFTQLTN KALSIVYFDL KRRSLPVEAL 360
  361 KYDNKFNIWV YQSEPDSSLK NVTSPFDDRY KQLEKLRLLV LKKFNKTERG TLLKYRVNQL 420
  421 SPGFFQRAGN DFKLILNEAS VSIQTCFKTN NITRLTSWTV ILGRLACLES EKFSGTLPNS 480
  481 TKDMDNWYVC HLCDIEKTGN PFVRINPNRP EAAGKSEIFR ILHSNFLSHP NIDEFSESLL 540
  541 SGILFSLHRI FSHFQ

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [556-974]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PPKLTDGNGQ INKSFKLVQK CFMNSNRYLR LLSTRIIPLF NISDSHNSED EHTATLIKFL  60
   61 QSQKLPVVKE NLVIAWTQLT LTTSNDVFDT LLLKLIDIFN SDDYSLRIMM TLQIKNMAKI 120
  121 LKKTPYQLLS PILPVLLRQL GKNLVERKVG FQNLIELLGY SSKTILDIFQ RYIIPYAIIQ 180
  181 YKSDVLSEIA KIMCDGDTSL INQMKVNLLK KNSRQIFAVA LVKHGLFSLD ILETLFLNRA 240
  241 PTFDKGYITA YLPDYKTLAE ITKLYKNSVT KDASDSENAN MILCSLRFLI TNFEKDKRHG 300
  301 SKYKNINNWT DDQEQAFQKK LQDNILGIFQ VFSSDIHDVE GRTTYYEKLR VINGISFLII 360
  361 YAPKKSIISA LAQISICLQT GLGLKEVRYE AFRCWHLLVR HLNDEELSTV IDSLIAFIL

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3

Region A:
Residues: [975-1241]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QKWSEFNGKL RNIVYSILDT LIKEKSDLIL KLKPYTTLAL VGKPELGILA RDGQFARMVN  60
   61 KIRSTTDLIP IFANNLKSSN KYVINQNLDD IEVYLRRKQT ERSIDFTPKK VGQTSDITLV 120
  121 LGALLDTSHK FRNLDKDLCE KCAKCISMIG VLDVTKHEFK RTTYSENEVY DLNDSVQTIK 180
  181 FLIWVINDIL VPAFWQSENP SKQLFVALVI QESLKYCGLS SESWDMNHKE LYPNEAKLWE 240
  241 KFNSVSKTTI YPLLSSLYLA QSWKEYV

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4

Region A:
Residues: [1242-1376]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PLKYPSNNFK EGYKIWVKRF TLDLLKTGTT ENHPLHVFSS LIREDDGSLS NFLLPYISLD  60
   61 IIIKAEKGTP YADILNGIII EFDSIFTCNL EGMNNLQVDS LRMCYESIFR VFEYCKKWAT 120
  121 EFKQNYSKLH GTFII

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

		MCM Score	GO Score	GO Term	SCOP Match	SCOP Description
View	Download	0.834	0.233	DNA damage checkpoint	a.25.1	Ferritin-like

Predicted functions:

Term	Confidence	Notes
binding	2.96084803796407	bayes_pls_golite062009
protein binding	2.66176697577398	bayes_pls_golite062009
nucleic acid binding	2.39117996950187	bayes_pls_golite062009
transcription regulator activity	1.93307188158984	bayes_pls_golite062009
DNA binding	1.77969838181048	bayes_pls_golite062009
protein transporter activity	1.51072955431931	bayes_pls_golite062009
protein kinase activity	1.40981221970766	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.19667256014399	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.18159704569055	bayes_pls_golite062009
kinase activity	1.155617721412	bayes_pls_golite062009
transcription factor activity	1.02508422532466	bayes_pls_golite062009
substrate-specific transporter activity	0.68280791022469	bayes_pls_golite062009
protein serine/threonine kinase activity	0.647706371567682	bayes_pls_golite062009
transferase activity	0.567560860286018	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.19278880370842	bayes_pls_golite062009
hydrolase activity	0.0962597719675237	bayes_pls_golite062009
clathrin binding	0.0923079381755934	bayes_pls_golite062009
transcription activator activity	0.061709024440191	bayes_pls_golite062009

Predicted Domain #5

Region A:
Residues: [1377-1817]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KDTKTTNMLL RIDEFLRTTP SDLLAQRSLE TDSFERSALY LEQCYRQNPH DKNQNGQLLK  60
   61 NLQITYEEIG DIDSLDGVLR TFATGNLVSK IEELQYSENW KLAQDCFNVL GKFSDDPKTT 120
  121 TRMLKSMYDH QLYSQIISNS SFHSSDGKIS LSPDVKEWYS IGLEAANLEG NVQTLKNWVE 180
  181 QIESLRNIDD REVLLQYNIA KALIAISNED PLRTQKYIHN SFRLIGTNFI TSSKETTLLK 240
  241 KQNLLMKLHS LYDLSFLSSA KDKFEYKSNT TILDYRMERI GADFVPNHYI LSMRKSFDQL 300
  301 KMNEQADADL GKTFFTLAQL ARNNARLDIA SESLMHCLER RLPQAELEFA EILWKQGEND 360
  361 RALKIVQEIH EKYQENSSVN ARDRAAVLLK FTEWLDLSNN SASEQIIKQY QDIFQIDSKW 420
  421 DKPYYSIGLY YSRLLERKKA E

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	68.026872
Match:	PF02259
Description:	FAT domain

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #6

Region A:
Residues: [1818-1972]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GYITNGRFEY RAISYFLLAF EKNTAKVREN LPKVITFWLD IAAASISEAP GNRKEMLSKA  60
   61 TEDICSHVEE ALQHCPTYIW YFVLTQLLSR LLHSHQSSAQ IIMHILLSLA VEYPSHILWY 120
  121 ITALVNSNSS KRVLRGKHIL EKYRQHSQNP HDLVS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	166.9897
Match:	1e8yA_
Description:	Phoshoinositide 3-kinase (PI3K) helical domain; Phoshoinositide 3-kinase (PI3K); Phoshoinositide 3-kinase (PI3K), catalytic domain
Matching Structure (courtesy of the PDB):

Predicted Domain #7

Region A:
Residues: [1973-2133]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SALDLTKALT RVCLQDVKSI TSRSGKSLEK DFKFDMNVAP SAMVVPVRKN LDIISPLESN  60
   61 SMRGYQPFRP VVSIIRFGSS YKVFSSLKKP KQLNIIGSDG NIYGIMCKKE DVRQDNQYMQ 120
  121 FATTMDFLLS KDIASRKRSL GINIYSVLSL REDCGILEMV P

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [2294-2322]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KNEVALMNVI ETIMYDRNMD HSIQKALKV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	166.9897
Match:	1e8yA_
Description:	Phoshoinositide 3-kinase (PI3K) helical domain; Phoshoinositide 3-kinase (PI3K); Phoshoinositide 3-kinase (PI3K), catalytic domain
Matching Structure (courtesy of the PDB):

Predicted Domain #8

Region A:
Residues: [2134-2293]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NVVTLRSILS TKYESLKIKY SLKSLHDRWQ HTAVDGKLEF YMEQVDKFPP ILYQWFLENF  60
   61 PDPINWFNAR NTYARSYAVM AMVGHILGLG DRHCENILLD IQTGKVLHVD FDCLFEKGKR 120
  121 LPVPEIVPFR LTPNLLDALG IIGTEGTFKK SSEVTLALMR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [2323-2368]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LRNKIRGIDP QDGLVLSVAG QTETLIQEAT SEDNLSKMYI GWLPFW

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	166.9897
Match:	1e8yA_
Description:	Phoshoinositide 3-kinase (PI3K) helical domain; Phoshoinositide 3-kinase (PI3K); Phoshoinositide 3-kinase (PI3K), catalytic domain
Matching Structure (courtesy of the PDB):

Predicted Domain #9

Region A:
Residues: [1644-1820]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SNTTILDYRM ERIGADFVPN HYILSMRKSF DQLKMNEQAD ADLGKTFFTL AQLARNNARL  60
   61 DIASESLMHC LERRLPQAEL EFAEILWKQG ENDRALKIVQ EIHEKYQENS SVNARDRAAV 120
  121 LLKFTEWLDL SNNSASEQII KQYQDIFQID SKWDKPYYSI GLYYSRLLER KKAEGYI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	5.69897
Match:	2fo7A
Description:	Crystal structure of an 8 repeat consensus TPR superhelix (trigonal crystal form)
Matching Structure (courtesy of the PDB):

Predicted Domain #10

Region A:
Residues: [1821-2368]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TNGRFEYRAI SYFLLAFEKN TAKVRENLPK VITFWLDIAA ASISEAPGNR KEMLSKATED  60
   61 ICSHVEEALQ HCPTYIWYFV LTQLLSRLLH SHQSSAQIIM HILLSLAVEY PSHILWYITA 120
  121 LVNSNSSKRV LRGKHILEKY RQHSQNPHDL VSSALDLTKA LTRVCLQDVK SITSRSGKSL 180
  181 EKDFKFDMNV APSAMVVPVR KNLDIISPLE SNSMRGYQPF RPVVSIIRFG SSYKVFSSLK 240
  241 KPKQLNIIGS DGNIYGIMCK KEDVRQDNQY MQFATTMDFL LSKDIASRKR SLGINIYSVL 300
  301 SLREDCGILE MVPNVVTLRS ILSTKYESLK IKYSLKSLHD RWQHTAVDGK LEFYMEQVDK 360
  361 FPPILYQWFL ENFPDPINWF NARNTYARSY AVMAMVGHIL GLGDRHCENI LLDIQTGKVL 420
  421 HVDFDCLFEK GKRLPVPEIV PFRLTPNLLD ALGIIGTEGT FKKSSEVTLA LMRKNEVALM 480
  481 NVIETIMYDR NMDHSIQKAL KVLRNKIRGI DPQDGLVLSV AGQTETLIQE ATSEDNLSKM 540
  541 YIGWLPFW

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	105.0
Match:	1e8yA
Description:	Phoshoinositide 3-kinase (PI3K) helical domain; Phoshoinositide 3-kinase (PI3K); Phoshoinositide 3-kinase (PI3K), catalytic domain
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: