YRC Public Data Repository - Protein Structure Prediction

Protein:	PKC1
Organism:	Saccharomyces cerevisiae
Length:	1151 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PKC1.

Description	E-value	Query Range	Subject Range
KPC1_CANAX - Protein kinase C-like 1 OS=Candida albicans GN=PKC1 PE=3 SV=1	0.0	[2..1148]	[11..1094]
gi\|6102720 - gi\|6102720\|emb\|CAB59301.1\| protein kinase C [Botryotinia fuckeliana]	0.0	[2..1147]	[4..1168]
gi\|12005625 - gi\|12005625\|gb\|AAG44542.1\|AF247001_1 protein kinase C [Blumeria graminis]	0.0	[2..1149]	[3..1157]
KPC1_COCHE - Protein kinase C-like OS=Cochliobolus heterostrophus GN=PKC1 PE=3 SV=1	0.0	[2..1147]	[3..1172]
gi\|172177 - gi\|172177\|gb\|AAA34878.1\| protein kinase C-like protein (PKC1)	0.0	[1..1151]	[1..1151]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[2..1148]	[11..1094]
PRKCA - protein kinase C, alpha gi\|190692009, gi... - gi\|254071371\|gb\|ACT64445.1\| protein kinase C, alpha protein [synthetic construct], gi\|190692009\|gb\|A... HGNC:9393\|MIM:17... - kinase C, alpha	0.0	[469..1149]	[23..663]
KPCA_RAT - Protein kinase C alpha type OS=Rattus norvegicus GN=Prkca PE=1 SV=3 gi\|56914 - gi\|56914\|emb\|CAA30266.1\| unnamed protein product [Rattus rattus]	0.0	[469..1149]	[23..663]

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Predicted Domain #1

Region A:
Residues: [1-95]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFSQLEQNI KKKIAVEENI IRGASALKKK TSNVMVIQKC NTNIREARQN LEYLEDSLKK  60
   61 LRLKTAQQSQ GENGSEDNER FNSKEYGFLS TKSPN

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	21.0
Match:	PF02185
Description:	Hr1 repeat

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [96-192]

      1          11         21         31         41         51         
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    1 EHIFSRLDLV KYDCPSLAQR IQYMLQQLEF KLQVEKQYQE ANTKLTKLYQ IDGDQRSSSA  60
   61 AEGGAMESKY RIQMLNKALK KYQAINVDFD QFKHQPN

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	9.8
Match:	1cxzB
Description:	Effector domain of the protein kinase pkn/prk1
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [193-333]

      1          11         21         31         41         51         
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    1 DIMDNQQPKF RRKQLTGVLT IGITAARDVD HIQSPMFARK PESYVTIKID DTIKARTKPS  60
   61 RNDRWSEDFQ IPVEKGNEIE ITVYDKVNDS LIPVAIMWLL LSDIAEEIRK KKAGQTNEQQ 120
  121 GWVNASNING GSSLASEEGS T

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	20.221849
Match:	1gmiA_
Description:	Domain from protein kinase C epsilon
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [334-387]

      1          11         21         31         41         51         
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    1 LTSTYSNSAI QSTSAKNVQG ENTSTSQIST NSWFVLEPSG QILLTLGFHK SSQI

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.764	b.34.2	SH3-domain
View	Download	0.673	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.658	b.34.2	SH3-domain
View	Download	0.607	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.568	d.9.2	Description not found.
View	Download	0.551	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.550	a.35.1	lambda repressor-like DNA-binding domains
View	Download	0.529	d.9.2	Description not found.
View	Download	0.510	b.34.2	SH3-domain
View	Download	0.503	d.17.3	Disulfide bond isomerase, DsbC, N-terminal domain
View	Download	0.499	d.9.2	Description not found.
View	Download	0.492	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.491	b.34.3	Myosin S1 fragment, N-terminal domain
View	Download	0.477	d.40.1	CI-2 family of serine protease inhibitors
View	Download	0.465	d.9.2	Description not found.
View	Download	0.452	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.441	b.34.7	DNA-binding domain of retroviral integrase
View	Download	0.417	d.9.2	Description not found.
View	Download	0.415	b.34.11	GW domain
View	Download	0.414	d.9.1	Interleukin 8-like chemokines
View	Download	0.413	b.34.3	Myosin S1 fragment, N-terminal domain
View	Download	0.375	b.34.5	Translation proteins SH3-like domain
View	Download	0.371	g.14.1	Kringle-like
View	Download	0.370	d.40.1	CI-2 family of serine protease inhibitors
View	Download	0.357	b.34.1	C-terminal domain of transcriptional repressors
View	Download	0.314	g.27.1	Fibronectin type I module
View	Download	0.280	b.11.1	gamma-Crystallin-like
View	Download	0.268	b.34.1	C-terminal domain of transcriptional repressors
View	Download	0.247	b.34.2	SH3-domain
View	Download	0.231	b.5.1	alpha-Amylase inhibitor tendamistat
View	Download	0.229	b.34.2	SH3-domain
View	Download	0.226	b.34.1	C-terminal domain of transcriptional repressors
View	Download	0.212	b.5.1	alpha-Amylase inhibitor tendamistat

Predicted functions:

Term	Confidence	Notes
binding	2.71288992341677	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	2.07238897772131	bayes_pls_golite062009
transcription regulator activity	2.02888738926089	bayes_pls_golite062009
kinase activity	2.00903309297046	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.83466699022067	bayes_pls_golite062009
protein kinase activity	1.61903873760762	bayes_pls_golite062009
nucleic acid binding	1.54948980710962	bayes_pls_golite062009
transferase activity	1.45394179515146	bayes_pls_golite062009
DNA binding	1.42056177729872	bayes_pls_golite062009
molecular transducer activity	1.25050856744516	bayes_pls_golite062009
signal transducer activity	1.25050856744516	bayes_pls_golite062009
protein binding	1.21002143294224	bayes_pls_golite062009
GTPase regulator activity	1.12412426198961	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.10707685935826	bayes_pls_golite062009
protein serine/threonine kinase activity	0.93378879973188	bayes_pls_golite062009
transcription factor activity	0.839889809493374	bayes_pls_golite062009
enzyme regulator activity	0.717794523788476	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.6091533221871	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.507633651875793	bayes_pls_golite062009
small GTPase regulator activity	0.442062495186646	bayes_pls_golite062009
kinase binding	0.409606963040614	bayes_pls_golite062009
catalytic activity	0.216721136299042	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.132870203047992	bayes_pls_golite062009

Predicted Domain #5

Region A:
Residues: [388-464]

      1          11         21         31         41         51         
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    1 ERKQLMGGLH RHGAIINRKE EIFEQHGHHF VQKSFYNIMC CAYCGDFLRY TGFQCQDCKF  60
   61 LCHKKCYTNV VTKCIAK

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	17.30103
Match:	PF00130
Description:	Phorbol esters/diacylglycerol binding domain (C1 domain)

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

Found no structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
binding	2.79128751898373	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	2.10625597744594	bayes_pls_golite062009
transcription regulator activity	2.07497340861199	bayes_pls_golite062009
kinase activity	2.03632850156074	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.8531437045627	bayes_pls_golite062009
protein kinase activity	1.62921423829738	bayes_pls_golite062009
nucleic acid binding	1.54797684894025	bayes_pls_golite062009
transferase activity	1.4550521488922	bayes_pls_golite062009
DNA binding	1.42215436462553	bayes_pls_golite062009
GTPase regulator activity	1.39167263636091	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.37139209958305	bayes_pls_golite062009
protein binding	1.29100556128076	bayes_pls_golite062009
signal transducer activity	1.25507073225414	bayes_pls_golite062009
molecular transducer activity	1.25507073225414	bayes_pls_golite062009
protein serine/threonine kinase activity	0.956859696337723	bayes_pls_golite062009
enzyme regulator activity	0.911061212692491	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.907368864863833	bayes_pls_golite062009
transcription factor activity	0.85055461881385	bayes_pls_golite062009
small GTPase regulator activity	0.661240850692236	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.6452191274371	bayes_pls_golite062009
kinase binding	0.494958567742569	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.38335246506929	bayes_pls_golite062009
catalytic activity	0.194464246602439	bayes_pls_golite062009
lipid binding	0.170968368793169	bayes_pls_golite062009

Predicted Domain #6

Region A:
Residues: [465-546]

      1          11         21         31         41         51         
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    1 TSTDTDPDEA KLNHRIPHRF LPTSNRGTKW CCHCGYILPW GRHKVRKCSE CGIMCHAQCA  60
   61 HLVPDFCGMS MEMANKILKT IQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	47.522879
Match:	1tbn__
Description:	Protein kinase c-gamma
Matching Structure (courtesy of the PDB):

Predicted Domain #7

Region A:
Residues: [547-627]

      1          11         21         31         41         51         
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    1 DTKRNQEKKK RTVPSAQLGS SIGTANGSDL SPSKLAERAN APLPPQPRKH DKTPSPQKVG  60
   61 RDSPTKQHDP IIDKKISLQT H

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.202	a.4.5	"Winged helix" DNA-binding domain

Predicted functions:

Term	Confidence	Notes
binding	2.77482286467294	bayes_pls_golite062009
phospholipid binding	2.74013385479058	bayes_pls_golite062009
neurexin binding	1.99616093461872	bayes_pls_golite062009
calcium-dependent phospholipid binding	1.85892769686753	bayes_pls_golite062009
lipid binding	1.79802914584378	bayes_pls_golite062009
protein binding	1.33917246836907	bayes_pls_golite062009
transporter activity	1.2628067945512	bayes_pls_golite062009
signal transducer activity	1.10701462579828	bayes_pls_golite062009
molecular transducer activity	1.10701462579828	bayes_pls_golite062009
substrate-specific transporter activity	0.816751946072324	bayes_pls_golite062009
receptor activity	0.516117489846513	bayes_pls_golite062009
SNARE binding	0.3731056408292	bayes_pls_golite062009
profilin binding	0.32399339703241	bayes_pls_golite062009

Predicted Domain #8

Region A:
Residues: [628-797]

      1          11         21         31         41         51         
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    1 GREKLNKFID ENEAYLNFTE GAQQTAEFSS PEKTLDPTSN RRSLGLTDLS IEHSQTWESK  60
   61 DDLMRDELEL WKAQREEMEL EIKQDSGEIQ EDLEVDHIDL ETKQKLDWEN KNDFREADLT 120
  121 IDSTHTNPFR DMNSETFQIE QDHASKEVLQ ETVSLAPTST HPSRTTDQQS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	12.154902
Match:	1dsyA_
Description:	C2 domain from protein kinase c (alpha)
Matching Structure (courtesy of the PDB):

Predicted Domain #9

Region A:
Residues: [798-873]

      1          11         21         31         41         51         
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    1 PQKSQTSTSA KHKKRAAKRR KVSLDNFVLL KVLGKGNFGK VILSKSKNTD RLCAIKVLKK  60
   61 DNIIQNHDIE SARAEK

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [890-906]

      1          11         21         31         41         51         
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    1 LYCSFQTENR IYFAMEF

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [1104-1151]

      1          11         21         31         41         51         
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    1 IKSPEDTSYF EQEFTSAPPT LTPLPSVLTT SQQEEFRGFS FMPDDLDL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	690.228787
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #10

Region A:
Residues: [874-889]

      1          11         21         31         41         51         
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    1 KVFLLATKTK HPFLTN

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [907-1103]

      1          11         21         31         41         51         
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    1 IGGGDLMWHV QNQRLSVRRA KFYAAEVLLA LKYFHDNGVI YRDLKLENIL LTPEGHIKIA  60
   61 DYGLCKDEMW YGNRTSTFCG TPEFMAPEIL KEQEYTKAVD WWAFGVLLYQ MLLCQSPFSG 120
  121 DDEDEVFNAI LTDEPLYPID MAGEIVQIFQ GLLTKDPEKR LGAGPRDADE VMEEPFFRNI 180
  181 NFDDILNLRV KPPYIPE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	690.228787
Match:	1fotA_
Description:	cAMP-dependent PK, catalytic subunit
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions:

Predicted functions: