YRC Public Data Repository - Protein Structure Prediction

Protein:	AFG1_YEAST
Organism:	Saccharomyces cerevisiae S288c
Length:	509 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for AFG1_YEAST.

Description	E-value	Query Range	Subject Range
AFG1_YEAST - Protein AFG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AFG1 PE=1 SV=1 AFG1 - Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochr...	0.0	[1..509]	[1..509]
N2B_HAEIR - Putative ATPase N2B OS=Haematobia irritans PE=2 SV=1	0.0	[9..506]	[11..459]
gi\|21593548, gi\|... - gi\|23198114\|gb\|AAN15584.1\| putative protein [Arabidopsis thaliana], gi\|21593548\|gb\|AAM65515.1\| unkno...	2.0E-93	[36..505]	[90..489]
CE16882 - status:Confirmed UniProt:Q95YE1 protein_id:AAK85448.1	5.0E-93	[24..503]	[8..431]
YBQ2_SCHPO - Uncharacterized protein C115.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC115.0... SPBC115.02c - AFG1 family mitochondrial ATPase	9.0E-90	[35..507]	[35..454]
gi\|25355785 - pir\|\|AC3269 probable ATPase N2B BMEI0136 [imported] - Brucella melitensis (strain 16M) gi\|17986420, gi\|... - gi\|17986420\|ref\|NP_539054.1\| putative ATPase n2B [Brucella melitensis 16M], gi\|17982014\|gb\|AAL51318.... gi\|225628128, gi... - gi\|225628128\|ref\|ZP_03786163.1\| ATPase n2B [Brucella ceti str. Cudo], gi\|225616953\|gb\|EEH14000.1\| AT...	2.0E-78	[28..458]	[21..395]
gi\|200388618, gi... - gi\|200388618\|ref\|ZP_03215230.1\| ATPase, AFG1 family [Salmonella enterica subsp. enterica serovar Vir... gi\|16766641, gi\|... - gi\|16766641\|ref\|NP_462256.1\| putative ATPase [Salmonella typhimurium LT2], gi\|16421905\|gb\|AAL22215.1... gi\|205328108, gi... - gi\|205328108\|gb\|EDZ14872.1\| ATPase, AFG1 family [Salmonella enterica subsp. enterica serovar 4,[5],1... lcl\|NC_016856.1_... - [gene=yhcM] [protein=putative ATPase] [protein_id=YP_005239530.1] [location=complement(3527441..3528... gi\|62129487, gi\|... - gi\|62181854\|ref\|YP_218271.1\| putative ATPase [Salmonella enterica subsp. enterica serovar Choleraesu...	4.0E-78	[34..461]	[4..371]

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Predicted Domain #1

Region A:
Residues: [1-252]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR DDTYQRGIIS  60
   61 SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG KPKNIGAYVD VSKIGNSIPR 120
  121 GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK RIHFHQFMQY VHKRSHEIVR EQNLKELGDA 180
  181 KGKEIDTVPF LAAEIANNSH VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR 240
  241 HPDELYINGV QR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	56.30103
Match:	1e32A_
Description:	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [253-356]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QSFIPCIELI KHRTKVIFLN SPTDYRKIPR PVSSVYYFPS DTSIKYASKE CKTRRETHIK  60
   61 EWYNYFAQAS HTDDSTDSHT VHKTFYDYPL TIWGREFKVP KCTP

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	56.30103
Match:	1e32A_
Description:	Membrane fusion atpase p97 N-terminal domain , P97-Nn; Membrane fusion atpase p97, D1 domain; Membrane fusion atpase p97 domain 2, P97-Nc
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.74389108884083	bayes_pls_golite062009
pyrophosphatase activity	2.67097555143215	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.65161658635465	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.64849912301318	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
primary active transmembrane transporter activity	2.39629638016861	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.39600931083427	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.36051733655256	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.35906629925754	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.35679406876731	bayes_pls_golite062009
hydrolase activity	2.32318595170495	bayes_pls_golite062009
ATPase activity	2.2625504298178	bayes_pls_golite062009
ATPase activity, coupled	2.24901251684901	bayes_pls_golite062009
binding	2.0681607836408	bayes_pls_golite062009
substrate-specific transporter activity	1.65609907941094	bayes_pls_golite062009
protein transporter activity	1.37245678118001	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.3182108176243	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
motor activity	0.70874549510465	bayes_pls_golite062009
ion transmembrane transporter activity	0.704934361725919	bayes_pls_golite062009
guanyl nucleotide binding	0.640772201693186	bayes_pls_golite062009
guanyl ribonucleotide binding	0.63468044350616	bayes_pls_golite062009
GTP binding	0.621689261700018	bayes_pls_golite062009
protein transmembrane transporter activity	0.46552249844591	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
nucleic acid binding	0.318452816722389	bayes_pls_golite062009
cation transmembrane transporter activity	0.282397764628576	bayes_pls_golite062009
protein binding	0.193768631623192	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [357-509]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PRVAQFTFKQ LCGEPLAAGD YLTLAKNFEA FIVTDIPYLS IYVRDEVRRF ITFLDAVYDS  60
   61 GGKLATTGAA DFSSLFVEPE QILNDFELRP TTKEPDSVDT GMVDEMVEKH GFSKEIAKKS 120
  121 QMFALDEERF AFARALSRLS QMSSTDWVTK PTY

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: