YRC Public Data Repository - Protein Structure Prediction

Protein:	FADJ_ECOBW
Organism:	Escherichia coli BW2952
Length:	714 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for FADJ_ECOBW.

Description	E-value	Query Range	Subject Range
FADJ_ECOHS - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O9:H4 (strain HS) GN=fadJ PE=3 SV=1	733.0	[0..1]	[708..1]
gi\|217327205, gi... - gi\|217327205\|ref\|ZP_03443288.1\| fatty oxidation complex, alpha subunit FadJ [Escherichia coli O157:H... gi\|189368241, gi... - gi\|189368241\|gb\|EDU86657.1\| fatty oxidation complex, alpha subunit FadJ [Escherichia coli O157:H7 st... gi\|13362694, gi\|... - gi\|15832478\|ref\|NP_311251.1\| multifunctional fatty acid oxidation complex subunit alpha [Escherichia... gi\|25291852, gi\|... - pir\|\|A85876 probable enzyme Z3604 [imported] - Escherichia coli (strain O157:H7, substrain EDL933),... gi\|15802887, gi\|... - gi\|15802887\|ref\|NP_288914.1\| multifunctional fatty acid oxidation complex subunit alpha [Escherichia... FADJ_ECO57 - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O157:H7 GN=fadJ PE=3 SV=1	718.0	[0..1]	[708..1]
gi\|193069764, gi... - gi\|75235545\|ref\|ZP_00719736.1\| COG1250: 3-hydroxyacyl-CoA dehydrogenase [Escherichia coli E110019], ...	718.0	[0..1]	[708..1]
gi\|191165478, gi... - gi\|75231394\|ref\|ZP_00717782.1\| COG1250: 3-hydroxyacyl-CoA dehydrogenase [Escherichia coli B7A], gi\|1...	717.0	[0..1]	[708..1]
gi\|47600725 - gi\|47600725\|emb\|CAE55846.1\| putative fatty acid oxidation complex alpha subunit [Escherichia coli Ni... FADJ_ECOL6 - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / ... FADJ_ECOL6 - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O6 GN=fadJ PE=3 SV=1 gi\|47600725 - gi\|47600725\|emb\|CAE55846.1\| putative fatty acid oxidation complex alpha subunit [Escherichia coli Ni... gi\|227835750, gi... - gi\|227887400\|ref\|ZP_04005205.1\| 3-hydroxybutyryl-CoA epimerase [Escherichia coli 83972], gi\|22783575...	716.0	[0..1]	[708..1]
FADJ_ECOK1 - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O1:K1 / APEC GN=fadJ PE=3 SV=1 gi\|226901186, gi... - gi\|237704820\|ref\|ZP_04535301.1\| multifunctional fatty acid oxidation complex subunit alpha [Escheric... FADJ_ECOUT - Fatty acid oxidation complex subunit alpha OS=Escherichia coli (strain UTI89 / UPEC) GN=fadJ PE=3 SV...	715.0	[0..1]	[708..1]
gi\|188491558, gi... - gi\|75515705\|ref\|ZP_00737840.1\| COG1250: 3-hydroxyacyl-CoA dehydrogenase [Escherichia coli 53638], gi...	715.0	[0..1]	[708..1]
gi\|30042017, gi\|... - gi\|30063762\|ref\|NP_837933.1\| multifunctional fatty acid oxidation complex subunit alpha [Shigella fl... FADJ_SHIFL - Fatty acid oxidation complex subunit alpha OS=Shigella flexneri GN=fadJ PE=3 SV=1 gi\|24052784, gi\|... - gi\|24113713\|ref\|NP_708223.1\| multifunctional fatty acid oxidation complex subunit alpha [Shigella fl... FADJ_SHISS - Fatty acid oxidation complex subunit alpha OS=Shigella sonnei (strain Ss046) GN=fadJ PE=3 SV=1	715.0	[0..1]	[708..1]
FADJ_SHIF8 - Fatty acid oxidation complex subunit alpha OS=Shigella flexneri serotype 5b (strain 8401) GN=fadJ PE...	715.0	[0..1]	[708..1]
FADJ_ECO24 - Fatty acid oxidation complex subunit alpha OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=f...	714.0	[0..1]	[708..1]

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Predicted Domain #1

Region A:
Residues: [1-714]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEMTSAFTLN VRLDNIAVIT IDVPGEKMNT LKAEFASQVR AIIKQLRENK ELRGVVFVSA  60
   61 KPDNFIAGAD INMIGNCKTA QEAEALARQG QQLMAEIHAL PIQVIAAIHG ACLGGGLELA 120
  121 LACHGRVCTD DPKTVLGLPE VQLGLLPGSG GTQRLPRLIG VSTALEMILT GKQLRAKQAL 180
  181 KLGLVDDVVP HSILLEAAVE LAKKERPSSR PLPVRERILA GPLGRALLFK MVGKKTEHKT 240
  241 QGNYPATERI LEVVETGLAQ GTSSGYDAEA RAFGELAMTP QSQALRSIFF ASTDVKKDPG 300
  301 SDAPPAPLNS VGILGGGLMG GGIAYVTACK AGIPVRIKDI NPQGINHALK YSWDQLEGKV 360
  361 RRRHLKASER DKQLALISGT TDYRGFAHRD LIIEAVFENL ELKQQMVAEV EQNCAAHTIF 420
  421 ASNTSSLPIG DIAAHATRPE QVIGLHFFSP VEKMPLVEII PHAGTSAQTI ATTVKLAKKQ 480
  481 GKTPIVVRDK AGFYVNRILA PYINEAIRML TQGERVEHID AALVKFGFPV GPIQLLDEVG 540
  541 IDTGTKIIPV LEAAYGERFS APANVVSSIL NDDRKGRKNG RGFYLYGQKG RKSKKQVDPA 600
  601 IYPLIGTQGQ GRISAPQVAE RCVMLMLNEA VRCVDEQVIR SVRDGDIGAV FGIGFPPFLG 660
  661 GPFRYIDSLG AGEVVAIMQR LATQYGSRFT PCERLVEMGA RGESFWKTTA TDLQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	175.0
Match:	1wdkA
Description:	fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native2)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
long-chain-3-hydroxyacyl-CoA dehydrogenase activity	4.80462645980977	bayes_pls_golite062009
3-hydroxybutyryl-CoA epimerase activity	4.30714786835318	bayes_pls_golite062009
3-hydroxyacyl-CoA dehydratase activity	4.29069790566708	bayes_pls_golite062009
long-chain-enoyl-CoA hydratase activity	4.09442487131529	bayes_pls_golite062009
racemase and epimerase activity, acting on hydroxy acids and derivatives	4.03462681763369	bayes_pls_golite062009
CoA carboxylase activity	3.89913842968597	bayes_pls_golite062009
acetyl-CoA carboxylase activity	3.85567083340773	bayes_pls_golite062009
acetyl-CoA C-acyltransferase activity	3.6090947563188	bayes_pls_golite062009
ligase activity, forming carbon-carbon bonds	3.4980371783229	bayes_pls_golite062009
oxidoreductase activity	3.09625241707629	bayes_pls_golite062009
3-hydroxyacyl-CoA dehydrogenase activity	3.06391460989761	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	3.02758902218302	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	1.65338499044318	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.47309297191111	bayes_pls_golite062009
propionyl-CoA carboxylase activity	1.20853698666984	bayes_pls_golite062009
phosphogluconate dehydrogenase (decarboxylating) activity	1.10855899772836	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.788034697021177	bayes_pls_golite062009
enoyl-CoA hydratase activity	0.439912268968251	bayes_pls_golite062009
binding	0.116896827329077	bayes_pls_golite062009
fatty acid synthase activity	0.0962368786590111	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: