YRC Public Data Repository - Protein Structure Prediction

Protein:	KEFC_ECOBW
Organism:	Escherichia coli BW2952
Length:	620 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for KEFC_ECOBW.

Description	E-value	Query Range	Subject Range
gi\|115511461, gi... - gi\|117622337\|ref\|YP_851250.1\| glutathione-regulated potassium-efflux system protein KefC [Escherichi...	405.0	[0..1]	[620..25]
gi\|253976282, gi... - gi\|254286975\|ref\|YP_003052723.1\| glutathione-regulated potassium-efflux system protein [Escherichia ... gi\|251783613, gi... - gi\|251783613\|ref\|YP_002997917.1\| hypothetical protein B21_00050 [Escherichia coli BL21], gi\|24237588... gi\|253972073, gi... - gi\|254160172\|ref\|YP_003043280.1\| glutathione-regulated potassium-efflux system protein [Escherichia ... gi\|226840570, gi... - gi\|254037464\|ref\|ZP_04871541.1\| potassium:proton antiporter [Escherichia sp. 1_1_43], gi\|226840570\|g... KEFC_ECO8A - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O8 (strain IAI1) GN=k... KEFC_ECOSE - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli (strain SE11) GN=kefC... KEFC_ECOHS - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O9:H4 (strain HS) GN=... KEFC_SHIBS - Glutathione-regulated potassium-efflux system protein KefC OS=Shigella boydii serotype 4 (strain Sb2... gi\|83584908, gi\|... - gi\|83584908\|ref\|ZP_00923565.1\| COG0475: Kef-type K+ transport systems, membrane components [Escheric... KEFC_SHIB3 - Glutathione-regulated potassium-efflux system protein KefC OS=Shigella boydii serotype 18 (strain CD...	398.0	[0..1]	[620..1]
KEFC_SHISS - Glutathione-regulated potassium-efflux system protein KefC OS=Shigella sonnei (strain Ss046) GN=kefC...	398.0	[0..1]	[620..1]
KEFC_ECO55 - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli (strain 55989 / EAEC)... KEFC_ECO24 - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O139:H28 (strain E243...	398.0	[0..1]	[620..1]
gi\|75255702, gi\|... - gi\|75255702\|ref\|ZP_00727468.1\| COG0475: Kef-type K+ transport systems, membrane components [Escheric...	398.0	[0..1]	[620..1]
gi\|194412085, gi... - gi\|75208903\|ref\|ZP_00709180.1\| COG0475: Kef-type K+ transport systems, membrane components [Escheric...	397.0	[0..1]	[620..1]
KEFC_ECOL6 - Glutathione-regulated potassium-efflux system protein kefC OS=Escherichia coli O6 GN=kefC PE=3 SV=1 KEFC_ECOL6 - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O6:H1 (strain CFT073 ... gi\|227885054, gi... - gi\|227885054\|ref\|ZP_04002859.1\| CPA2 family potassium:proton (K+:H+) antiporter [Escherichia coli 83...	397.0	[0..1]	[620..1]
KEFC_ECOUT - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli (strain UTI89 / UPEC)... KEFC_ECO45 - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O45:K1 (strain S88 / ... gi\|226902103, gi... - gi\|237704191\|ref\|ZP_04534672.1\| glutathione-regulated potassium-efflux system protein KefC [Escheric...	397.0	[0..1]	[620..1]
gi\|191174104, gi... - gi\|75237160\|ref\|ZP_00721208.1\| COG0475: Kef-type K+ transport systems, membrane components [Escheric... KEFC_ECOL5 - Glutathione-regulated potassium-efflux system protein KefC OS=Escherichia coli O6:K15:H31 (strain 53...	397.0	[0..1]	[620..1]
KEFC_SHIFL - Glutathione-regulated potassium-efflux system protein KefC OS=Shigella flexneri GN=kefC PE=3 SV=1 gi\|30039856, gi\|... - gi\|30061614\|ref\|NP_835785.1\| glutathione-regulated potassium-efflux system protein KefC [Shigella fl... gi\|24050245, gi\|... - gi\|24111493\|ref\|NP_706003.1\| glutathione-regulated potassium-efflux system protein KefC [Shigella fl... gi\|209746940 - gi\|209746940\|gb\|ACI71777.1\| glutathione-regulated K+ efflux antiporter [Escherichia coli]	397.0	[0..1]	[620..1]

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Predicted Domain #1

Region A:
Residues: [1-192]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSHTLIQAL IYLGSAALIV PIAVRLGLGS VLGYLIAGCI IGPWGLRLVT DAESILHFAE  60
   61 IGVVLMLFII GLELDPQRLW KLRAAVFGCG ALQMVICGGL LGLFCMLLGL RWQVAELIGM 120
  121 TLALSSTAIA MQAMNERNLM VTQMGRSAFA VLLFQDIAAI PLVAMIPLLA TSSASTTMGA 180
  181 FALSALKVAG AL

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [193-284]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VLVVLLGRYV TRPALRFVAR SGLREVFSAV ALFLVFGFGL LLEEVGLSMA MGAFLAGVLL  60
   61 ASSEYRHALE SDIEPFKGLL LGLFFIGVGM SI

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3

Region A:
Residues: [285-620]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DFGTLLENPL RIVILLLGFL IIKIAMLWLI ARPLQVPNKQ RRWFAVLLGQ GSEFAFVVFG  60
   61 AAQMANVLEP EWAKSLTLAV ALSMAATPIL LVILNRLEQS STEEAREADE IDEEQPRVII 120
  121 AGFGRFGQIT GRLLLSSGVK MVVLDHDPDH IETLRKFGMK VFYGDATRMD LLESAGAAKA 180
  181 EVLINAIDDP QTNLQLTEMV KEHFPHLQII ARARDVDHYI RLRQAGVEKP ERETFEGALK 240
  241 TGRLALESLG LGPYEARERA DVFRRFNIQM VEEMAMVEND TKARAAVYKR TSAMLSEIIT 300
  301 EDREHLSLIQ RHGWQGTEEG KHTGNMADEP ETKPSS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	17.69897
Match:	1lnqA
Description:	Potassium channel-related protein MthK
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
potassium channel activity	7.45561375804942	bayes_pls_golite062009
voltage-gated potassium channel activity	7.44058571110681	bayes_pls_golite062009
voltage-gated cation channel activity	7.3386698127529	bayes_pls_golite062009
voltage-gated ion channel activity	7.16469552587678	bayes_pls_golite062009
voltage-gated channel activity	7.14063840809955	bayes_pls_golite062009
metal ion transmembrane transporter activity	5.93188092092616	bayes_pls_golite062009
cation channel activity	5.49362429102263	bayes_pls_golite062009
cation transmembrane transporter activity	5.13805155650973	bayes_pls_golite062009
inward rectifier potassium channel activity	5.0997110076666	bayes_pls_golite062009
ion transmembrane transporter activity	5.04374327766634	bayes_pls_golite062009
ion channel activity	4.88752793243149	bayes_pls_golite062009
gated channel activity	4.87650815860853	bayes_pls_golite062009
substrate-specific channel activity	4.76774891791883	bayes_pls_golite062009
channel activity	4.7460548761362	bayes_pls_golite062009
passive transmembrane transporter activity	4.7460548761362	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	4.64120459994573	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	3.88411419181428	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.96809690970616	bayes_pls_golite062009
ligand-gated ion channel activity	2.94075163359893	bayes_pls_golite062009
ligand-gated channel activity	2.94075163359893	bayes_pls_golite062009
transporter activity	2.93091397928047	bayes_pls_golite062009
transmembrane transporter activity	2.82224868778929	bayes_pls_golite062009
substrate-specific transporter activity	2.79688978937497	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	2.13014428099026	bayes_pls_golite062009
potassium ion transmembrane transporter activity	1.34449386905866	bayes_pls_golite062009
catalytic activity	1.03099072397093	bayes_pls_golite062009
active transmembrane transporter activity	0.239024094835991	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: