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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: Sample: HX13 - trans-SNARE complex forms but fusion is blocked.

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 13 116 52.3% 0.0365 32498
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 14 77 46.5% 0.0217 36711
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 11 37 42.1% 0.0113 32803
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 7 11 35.2% 0.00387 28964
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 6 28 30.0% 0.0115 24668
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 5 9 21.9% 0.00219 40054
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 6 17 18.7% 0.00267 63004
[View Peptides]   PDX1 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... 3 6 16.8% 0.0013 45362
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 8 18 16.4% 0.00333 51818
[View Peptides]   HYP2 Translation elongation factor eIF-5A, previously thought to function in translation initiation; simi... 2 2 14.0% 0.00113 17114
[View Peptides]   SEC18 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... 6 13 13.9% 0.00153 84056
[View Peptides]   HOC1 Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localize... 2 3 13.6% 6.75E-4 46297
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 6 18 13.3% 0.00235 74468
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 12 19 12.6% 0.00115 166749
[View Peptides]   KTR4 Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltran... 3 3 12.5% 5.76E-4 54556
[View Peptides]   PRM5 Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrit... 2 2 11.9% 5.6E-4 34651
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 3 3 11.4% 5.34E-4 54545
[View Peptides]   PCL1 Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved... 2 2 10.4% 6.38E-4 32095
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 5 6 9.5% 6.36E-4 95529
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 3 6 9.4% 9.28E-4 64597
[View Peptides]   PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... 3 7 9.3% 0.00148 46343
[View Peptides]   ENA5 Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family 5 6 9.2% 4.9E-4 120296
[View Peptides]   ENA2 P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li... 5 6 9.2% 4.9E-4 120317
[View Peptides]   ENA1 P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance 5 6 9.2% 4.9E-4 120357
[View Peptides]   VPS16 Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... 4 4 9.0% 4.46E-4 92741
[View Peptides]   ZRT3 Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm whe... 2 2 8.2% 3.54E-4 55403
[View Peptides]   VBA4 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... 3 5 8.1% 5.8E-4 85683
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 5 6 7.8% 5.39E-4 113412
[View Peptides]   YCK3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... 2 3 7.8% 5.1E-4 60261
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 7 13 7.7% 6.17E-4 214849
[View Peptides]   IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... 2 3 6.8% 5.9E-4 49976
[View Peptides]   VTC3 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 4 17 6.6% 0.00181 96553
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 4 6 6.5% 5.09E-4 122881
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 3 6 6.5% 9.1E-4 64382
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 2 5 6.5% 8.53E-4 58227
[View Peptides]   PEP5 Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... 4 6 6.4% 5.19E-4 117477
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 3 6.4% 7.12E-4 41690
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 3 7 6.0% 7.17E-4 98712
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 7 10 5.6% 5.88E-4 171120
[View Peptides]   DAP2 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... 3 4 5.3% 4.36E-4 93404
[View Peptides]   HXT3 Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low ... 2 2 5.3% 3.14E-4 62558
[View Peptides]   KRE6 Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functional... 2 2 5.0% 2.47E-4 80123
[View Peptides]   SHM2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... 4 9 4.9% 0.00171 52219
[View Peptides]   HMG1 One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, whi... 3 3 4.3% 2.53E-4 115625
[View Peptides]   TFP1 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... 2 2 4.0% 1.66E-4 118637
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 2 3 4.0% 3.85E-4 77267
[View Peptides]   YBT1 Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... 3 4 3.3% 2.14E-4 189161
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 3 4 3.2% 3.68E-4 109736
[View Peptides]   YGR125W Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal ... 2 2 3.0% 1.72E-4 116971
[View Peptides]   PUF2 Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that ... 2 2 2.6% 1.66E-4 119508
[View Peptides]   PEP3 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... 2 2 2.6% 1.94E-4 107398
[View Peptides]   SEC27 Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport... 2 2 2.6% 2.0E-4 99445
[View Peptides]   VTH2 Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in ... 2 2 2.1% 1.15E-4 174401
[View Peptides]   VTH1 Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in ... 2 2 2.1% 1.15E-4 174427
[View Peptides]   VAC7 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... 2 2 1.6% 1.53E-4 128140

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