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Complex Overview

From Publication: Ho Y. et al. (2002) Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature. 2002 Jan 10;415(6868):180-3.
Notes: This molecular complex record represents a population of complexes co-purified by immunoprecipitation of FLAG-tagged Sps1. The topolgies of protein complexes in this experiment are unknown.
Complex Size: 18 proteins

Complex Member Proteins

Protein

GO Cellular Component GO Biological Process GO Molecular Function
AHA1
  • cytoplasm
  • protein folding
  • response to stress
  • chaperone binding
  • chaperone activator activity
  • ATPase activator activity
  • ARP2
  • mitochondrion
  • Arp2/3 protein complex
  • mitochondrion inheritance
  • actin filament organization
  • ATP binding
  • actin binding
  • structural constituent of cytoskeleton
  • ATP3
  • mitochondrial proton-transporting ATP synthase, central stalk
  • mitochondrion
  • ATP synthesis coupled proton transport
  • hydrogen ion transporting ATP synthase activity, rotational mechanism
  • CPR6
  • cytoplasm
  • protein folding
  • peptidyl-prolyl cis-trans isomerase activity
  • unfolded protein binding
  • IDH1
  • mitochondrial matrix
  • mitochondrion
  • mitochondrial nucleoid
  • glutamate biosynthetic process
  • isocitrate metabolic process
  • tricarboxylic acid cycle
  • isocitrate dehydrogenase (NAD+) activity
  • NMD5
  • nucleus
  • cytoplasm
  • protein import into nucleus
  • protein transmembrane transporter activity
  • PET10
  • lipid particle
  • aerobic respiration
  • molecular_function
  • PHO84
  • integral to plasma membrane
  • phosphate transport
  • manganese ion transport
  • inorganic phosphate transmembrane transporter activity
  • manganese ion transmembrane transporter activity
  • PPH21
  • protein phosphatase type 2A complex
  • mitotic cell cycle spindle assembly checkpoint
  • G1/S transition of mitotic cell cycle
  • protein amino acid dephosphorylation
  • actin filament organization
  • budding cell bud growth
  • translation
  • protein serine/threonine phosphatase activity
  • phosphoprotein phosphatase activity
  • metal ion binding
  • hydrolase activity
  • PPH22
  • condensed nuclear chromosome, centromeric region
  • protein phosphatase type 2A complex
  • mitotic cell cycle spindle assembly checkpoint
  • G1/S transition of mitotic cell cycle
  • protein amino acid dephosphorylation
  • actin filament organization
  • budding cell bud growth
  • translation
  • protein serine/threonine phosphatase activity
  • phosphoprotein phosphatase activity
  • metal ion binding
  • hydrolase activity
  • PRB1
  • fungal-type vacuole lumen
  • fungal-type vacuole
  • cellular response to starvation
  • sporulation resulting in formation of a cellular spore
  • vacuolar protein catabolic process
  • serine-type endopeptidase activity
  • REP1
  • nucleus
  • 2-micrometer plasmid partitioning
  • molecular_function
  • RPN8
  • proteasome regulatory particle, lid subcomplex
  • ubiquitin-dependent protein catabolic process
  • molecular_function
  • SDH2
  • mitochondrial respiratory chain complex II
  • mitochondrion
  • tricarboxylic acid cycle
  • mitochondrial electron transport, succinate to ubiquinone
  • succinate dehydrogenase (ubiquinone) activity
  • SPS1
  • nucleus
  • prospore membrane
  • cytoplasm
  • ascospore formation
  • protein amino acid phosphorylation
  • ascospore wall assembly
  • protein serine/threonine kinase activity
  • VMA8
  • vacuolar proton-transporting V-type ATPase, V1 domain
  • fungal-type vacuole membrane
  • vacuolar acidification
  • proton-transporting ATPase activity, rotational mechanism
  • VPS74
  • nucleus
  • cytoplasm
  • protein targeting to vacuole
  • molecular_function
  • YHR033W
  • cytoplasm
  • biological_process
  • molecular_function
  • Cellular Component Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    protein phosphatase type 2A complex 1.1517E-4 18 6 2 6292
    proton-transporting two-sector ATPase complex, catalytic domain 5.9184E-4 18 13 2 6292
    protein serine/threonine phosphatase complex 1.0249E-3 18 17 2 6292
    proton-transporting two-sector ATPase complex 4.3495E-3 18 35 2 6292
    mitochondrial proton-transporting ATP synthase, central stalk 8.5592E-3 18 3 1 6292
    proton-transporting ATP synthase, central stalk 8.5592E-3 18 3 1 6292

    Biological Process Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    phosphorus metabolic process 3.5509E-4 18 229 5 6292
    phosphate metabolic process 3.5509E-4 18 229 5 6292
    tricarboxylic acid cycle 7.9401E-4 18 15 2 6292
    acetyl-CoA catabolic process 7.9401E-4 18 15 2 6292
    actin filament organization 8.7722E-4 18 68 3 6292
    acetyl-CoA metabolic process 1.4247E-3 18 20 2 6292
    coenzyme catabolic process 1.4247E-3 18 20 2 6292
    aerobic respiration 1.5656E-3 18 83 3 6292
    negative regulation of nuclear division 1.572E-3 18 21 2 6292
    negative regulation of mitotic metaphase/anaphase transition 1.572E-3 18 21 2 6292
    mitotic cell cycle spindle assembly checkpoint 1.572E-3 18 21 2 6292
    negative regulation of mitosis 1.572E-3 18 21 2 6292
    spindle assembly checkpoint 1.572E-3 18 21 2 6292
    negative regulation of cell cycle 1.7262E-3 18 22 2 6292
    cofactor catabolic process 1.7262E-3 18 22 2 6292
    generation of precursor metabolites and energy 1.9454E-3 18 195 4 6292
    spindle checkpoint 2.2305E-3 18 25 2 6292
    regulation of mitotic metaphase/anaphase transition 2.2305E-3 18 25 2 6292
    mitotic cell cycle spindle checkpoint 2.2305E-3 18 25 2 6292
    cellular respiration 2.4501E-3 18 97 3 6292
    mitotic cell cycle checkpoint 2.7962E-3 18 28 2 6292
    protein amino acid dephosphorylation 2.7962E-3 18 28 2 6292
    regulation of nuclear division 2.9982E-3 18 29 2 6292
    negative regulation of organelle organization 2.9982E-3 18 29 2 6292
    negative regulation of cellular component organization 2.9982E-3 18 29 2 6292
    regulation of mitosis 2.9982E-3 18 29 2 6292
    negative regulation of cell cycle process 3.6443E-3 18 32 2 6292
    actin cytoskeleton organization 3.7809E-3 18 113 3 6292
    budding cell bud growth 4.1079E-3 18 34 2 6292
    actin filament-based process 4.1707E-3 18 117 3 6292
    mitotic metaphase/anaphase transition 4.8521E-3 18 37 2 6292
    dephosphorylation 5.6539E-3 18 40 2 6292
    oxidative phosphorylation 7.4266E-3 18 46 2 6292
    energy derivation by oxidation of organic compounds 8.1798E-3 18 149 3 6292
    G1/S transition of mitotic cell cycle 8.7307E-3 18 50 2 6292
    regulation of organelle organization 9.4186E-3 18 52 2 6292

    Molecular Function Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    protein serine/threonine phosphatase activity 2.4123E-3 18 26 2 6292
    inorganic cation transmembrane transporter activity 2.7496E-3 18 101 3 6292
    cation transmembrane transporter activity 4.2718E-3 18 118 3 6292
    isocitrate dehydrogenase (NAD+) activity 5.7138E-3 18 2 1 6292
    substrate-specific transmembrane transporter activity 6.0556E-3 18 267 4 6292
    phosphoprotein phosphatase activity 6.5123E-3 18 43 2 6292
    ion transmembrane transporter activity 7.7338E-3 18 146 3 6292
    chaperone activator activity 8.5592E-3 18 3 1 6292
    transmembrane transporter activity 9.7638E-3 18 306 4 6292

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