Protein Overview: EEB1

General Information:
Name(s) found:	EEB1 / YPL095C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325162\|ref\|NP_015230.1\| Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short chain esterase activity; may be involved in lipid metabolism and d [NCBI NR] gi\|51012573\|gb\|AAT92580.1\| YPL095C [Saccharomyces cerevisiae] [NCBI NR] gi\|2501571\|sp\|Q02891.1\|MCFS1_YEAST RecName: Full=Medium-chain fatty acid ethyl ester synthase/esterase 1; AltName: Full=Alcohol O-acetyltransferase; AltName: Full=Ethyl ester biosynthesis protein 1 [NCBI NR] pir\|\|S61971 hypothetical protein YPL095c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1151231\|gb\|AAB68204.1\| Lpg13p [NCBI NR] (Q02891) Hypothetical UPF0017 protein YPL095c [Swiss-Prot] (Q02891) UPF0017 protein YPL095C [Swiss-Prot] Medium-chain fatty acid ethyl ester synthase/esterase 1 OS=Saccharomyces cerevisiae GN=EEB1 PE=1 SV=1 [Swiss-Prot] EEB1 Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of short-chain fatty acid ethyl ester production during fermentation [mips] Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification [SGD] (Q02891) Hypothetical protein YPL095C [4932.FASTACCERV.txt] Chr XVI from 369436-368066, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XVI from 368066-369436, reverse complement [yeastorf3.fasta] YPL095C, Chr XVI from 368066-369436, reverse complement [yeast_orfs2] YPL095C SGDID:S0006016, Chr XVI from 369436-368066, reverse complement, Uncharacterized ORF [yeastflysequest.fasta] YPL095C SGDID:S0006016, Chr XVI from 369438-368068, reverse complement, Uncharacterized ORF [10112005cervSGD.fasta] Ypl095cp [Saccharomyces cerevisiae]gi\|51012573\|gb\|AAT92580.1\| YPL095C [Saccharomyces cerevisiae]gi\|2501571\|sp\|Q02891\|YP95_YEAST Hypothetical UPF0017 protein YPL095cgi\|1151231\|gb\|AAB68204.1\| Lpg13pgi\|2132185\|pir\|\|S61971 hypothetical protein YPL095c - y [nrdb_11152004_Chlamydomonas] YPL095C SGDID:S000006016, Chr XVI from 369438-368068, reverse complement, Uncharacterized ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of short-chain fatty acid ethyl ester production during fermentation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] EEB1 SGDID:S000006016, Chr XVI from 369438-368068, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short chain es [yeast-contam.fasta] EEB1 SGDID:S000006016, Chr XVI from 369438-368068, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain es [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] YPL095C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Lpg13p [nr-271106-contam.fasta] UPF0017 protein YPL095C [nr-271106-contam.fasta] Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short chain esterase activity; may be involved in lipid metabolism and detoxification; Eeb1p [Saccha [nr-271106-contam.fasta] EEB1 SGDID:S000006016, Chr XVI from 369438-368068, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification" [yeast-bovrabprots.fasta] EEB1 SGDID:S000006016, Chr XVI from 369438-368068, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short chain esterase activity; may be involved in lipid metabolism and detoxification" [yeast-contam_custom.fasta] [chemostatdb.fasta] Medium-chain fatty acid ethyl ester synthase/esterase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EEB1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] EEB1 SGDID:S000006016, Chr XVI from 369441-368071, Genome Release 64-1-1, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification" [yeast-030211-contam.fasta] EEB1 SGDID:S000006016, Chr XVI from 369441-368071, Genome Release 64-2-1, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YPL095C SGDID:S000006016, Chr XVI from 369441-368071, Genome Release 64-1-1, reverse complement, Verified ORF, "Acyl-coenzymeA:ethanol O-acyltransferase responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	456 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	medium-chain fatty acid metabolic process [IMP]
Molecular Function:	hydrolase activity, acting on ester bonds [IDA] alcohol O-acetyltransferase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFRSGYYPTV TPSHWGYNGT VKHVLGEKGT KSLAFRDSKR QIPLHEFVTK HVPTLKDGAN  60
   61 FRLNSLLFTG YLQTLYLSAG DFSKKFQVFY GREIIKFSDG GVCTADWVMP EWEQTYSLNA 120
  121 EKASFNEKQF SNDEKATHPK GWPRLHPRTR YLSSEELEKC HSKGYSYPLV VVLHGLAGGS 180
  181 HEPLIRALSE DLSKVGDGKF QVVVLNARGC SRSKVTTRRI FTALHTGDVR EFLNHQKALF 240
  241 PQRKIYAVGT SFGAAMLTNY LGEEGDNCPL NAAVALSNPW DFVHTWDKLA HDWWSNHIFS 300
  301 RTLTQFLTRT VKVNMNELQV PENFEVSHKP TVEKPVFYTY TRENLEKAEK FTDILEFDNL 360
  361 FTAPSMGLPD GLTYYRKASS INRLPNIKIP TLIINATDDP VTGENVIPYK QARENPCVLL 420
  421 CETDLGGHLA YLDNESNSWL TKQAAEFLGS FDELVL

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUD2	EEB1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..130]	MSA	1.19799	View MSA. No confident structure predictions are available.
2	View Details	[131..456]	PSI-BLAST	56.69897	Chloroperoxidase F

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
catalytic activity	1.39629727416851	bayes_pls_golite062009
lipase activity	0.849473630399459	bayes_pls_golite062009
carboxylesterase activity	0.424330502936163	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	0.264183693934251	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2