Protein Overview: NAR1

General Information:
Name(s) found:	NAR1 / YNL240C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324089\|ref\|NP_014159.1\| Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf; Nar1p [Saccharomyces cerevi [NCBI NR] gi\|51013435\|gb\|AAT93011.1\| YNL240C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S63206 LET1 protein homolog YNL240c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1730836\|sp\|P23503.2\|NAR1_YEAST RecName: Full=Nuclear architecture-related protein 1 [NCBI NR] gi\|1302274\|emb\|CAA96145.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1183974\|emb\|CAA93358.1\| N1114 [Saccharomyces cerevisiae] [NCBI NR] Cytosolic Fe-S cluster assembly factor NAR1 OS=Saccharomyces cerevisiae GN=NAR1 PE=1 SV=2 [Swiss-Prot] NAR1 Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins [mips] Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf [SGD] Chr XIV from 198501-199976, reverse complement [yeastorf3.fasta] Chr XIV from 199976-198501, reverse complement [yeastorf5.fasta] (P23503) Nuclear architecture related protein 1 [4932.FASTACCERV.txt] NAR1, Chr XIV from 198501-199976, reverse complement [yeast_orfs2] NAR1 SGDID:S0005184, Chr XIV from 199976-198501, reverse complement, Verified ORF [yeastflysequest.fasta] Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf (Nuclear prelamin A Recognition Factor); Nuclear architecture related protein with homology to [nrdb_11152004_Chlamydomonas] NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS prote [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxida [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] N1114 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf; Nar1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YNL240C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nuclear architecture-related protein 1 [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Nuclear architecture related protein; component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins; homologous to human Narf" [yeast-bovrabprots.fasta] NAR1 SGDID:S000005184, Chr XIV from 199978-198503, reverse complement, Verified ORF, "Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf" [scerevisihumanrev.fasta] [chemostatdb.fasta] Cytosolic Fe-S cluster assembly factor NAR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAR1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NAR1 SGDID:S000005184, Chr XIV from 199977-198502, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf" [yeast-030211-contam.fasta] NAR1 SGDID:S000005184, Chr XIV from 199977-198502, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery; required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; deficiency results in shortened lifespan and sensitivity to paraquat; homologous to human Narf" [20141208_orf_trans.fasta] YNL240C SGDID:S000005184, Chr XIV from 199977-198502, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	491 amino acids
Gene Ontology:
Cellular Component:	membrane [IDA] cytosol [IDA]
Biological Process:	iron-sulfur cluster assembly [IMP]
Molecular Function:	ferredoxin hydrogenase activity [ISS] metal ion binding [IEA] 4 iron, 4 sulfur cluster binding [IEA] iron-sulfur cluster binding [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSALLSESDL NDFISPALAC VKPTQVSGGK KDNVNMNGEY EVSTEPDQLE KVSITLSDCL  60
   61 ACSGCITSSE EILLSSQSHS VFLKNWGKLS QQQDKFLVVS VSPQCRLSLA QYYGLTLEAA 120
  121 DLCLMNFFQK HFQCKYMVGT EMGRIISISK TVEKIIAHKK QKENTGADRK PLLSAVCPGF 180
  181 LIYTEKTKPQ LVPMLLNVKS PQQITGSLIR ATFESLAIAR ESFYHLSLMP CFDKKLEASR 240
  241 PESLDDGIDC VITPREIVTM LQELNLDFKS FLTEDTSLYG RLSPPGWDPR VHWASNLGGT 300
  301 CGGYAYQYVT AVQRLHPGSQ MIVLEGRNSD IVEYRLLHDD RIIAAASELS GFRNIQNLVR 360
  361 KLTSGSGSER KRNITALRKR RTGPKANSRE MAAATAATAD PYHSDYIEVN ACPGACMNGG 420
  421 GLLNGEQNSL KRKQLVQTLN KRHGEELAMV DPLTLGPKLE EAAARPLSLE YVFAPVKQAV 480
  481 EKDLVSVGST W

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CIA1	NAR1	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..491]	PSI-BLAST	334.0103	Fe-only hydrogenase, catalytic domain; Fe-only hydrogenase, N-terminal domain; Fe-only hydrogenase, second domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	1.47874003627413	bayes_pls_golite062009
NADH dehydrogenase (ubiquinone) activity	1.31458462821219	bayes_pls_golite062009
iron-sulfur cluster binding	1.18054918348653	bayes_pls_golite062009
metal cluster binding	1.18054918348653	bayes_pls_golite062009
NADH dehydrogenase activity	1.10428419414285	bayes_pls_golite062009
lamin binding	0.863386378061341	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	0.778342926116907	bayes_pls_golite062009
catalytic activity	0.488483980994331	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	0.312816353615077	bayes_pls_golite062009
ferredoxin hydrogenase activity	0.278768281741128	bayes_pls_golite062009
oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor	0.278768281741128	bayes_pls_golite062009
protein binding	0.221871950373022	bayes_pls_golite062009
electron carrier activity	0.0554226204149908	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2