Protein Overview: MLS1

General Information:
Name(s) found:	MLS1 / YNL117W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324212\|ref\|NP_014282.1\| Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium; Mls [NCBI NR] gi\|400226\|sp\|P30952.1\|MASY_YEAST RecName: Full=Malate synthase 1, glyoxysomal [NCBI NR] gi\|3965\|emb\|CAA45750.1\| malate synthase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S26645 malate synthase (EC 4.1.3.2) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1302043\|emb\|CAA95997.1\| MLS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|125863508\|gb\|ABN58558.1\| MLS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|125863488\|gb\|ABN58540.1\| MLS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1183956\|emb\|CAA93390.1\| Malate synthase [Saccharomyces cerevisiae] [NCBI NR] Malate synthase 1, glyoxysomal OS=Saccharomyces cerevisiae GN=MLS1 PE=1 SV=1 [Swiss-Prot] MLS1 Malate synthase 1 [mips] Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium [SGD] MLS1, Chr XIV from 406355-408019 [yeast_orfs2] Chr XIV from 406355-408019 [yeastorf3.fasta] (P30952) Malate synthase 1, glyoxysomal (EC 2.3.3.9) [4932.FASTACCERV.txt] MLS1 SGDID:S0005061, Chr XIV from 406358-408022, Verified ORF [yeastflysequest.fasta] Mls1p [Saccharomyces cerevisiae]gi\|3965\|emb\|CAA45750.1\| malate synthase [Saccharomyces cerevisiae]gi\|1183956\|emb\|CAA93390.1\| Malate synthase [Saccharomyces cerevisiae]gi\|1302043\|emb\|CAA95997.1\| MLS1 [Saccharomyces cerevisiae]gi\|283194\|pir\|\|S26645 mala [nrdb_11152004_Chlamydomonas] MLS1 SGDID:S000005061, Chr XIV from 406360-408024, Verified ORF, "Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes d [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium; Mls1p [Saccharomyces cerevisiae [nr-271106-contam.fasta] Malate synthase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MLS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] malate synthase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Malate synthase 1, glyoxysomal [nr-271106-contam.fasta] MLS1 SGDID:S000005061, Chr XIV from 406360-408024, Verified ORF, "Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Malate synthase 1, glyoxysomal OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MLS1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MLS1 SGDID:S000005061, Chr XIV from 406358-408022, Genome Release 64-1-1, Verified ORF, "Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium" [yeast-030211-contam.fasta] MLS1 SGDID:S000005061, Chr XIV from 406358-408022, Genome Release 64-2-1, Verified ORF, "Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA" [20141208_orf_trans.fasta] YNL117W SGDID:S000005061, Chr XIV from 406358-408022, Genome Release 64-1-1, Verified ORF, "Malate synthase, enzyme of the glyoxylate cycle, involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth in oleic acid medium" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	554 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] peroxisomal matrix [TAS]
Biological Process:	glyoxylate cycle [TAS]
Molecular Function:	malate synthase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV  60
   61 VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV 120
  121 NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG 180
  181 WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF 240
  241 CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN 300
  301 DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK 360
  361 VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN 420
  421 GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD 480
  481 TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE 540
  541 IVSTKATPTD LSKL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		CAT2 CIT2 MDH3 MLS1 POT1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SOG2	MLS1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..104]	ORFEUS	9.93	Malate synthase G
2	View Details	[105..421]	PSI-BLAST	138.228787	Malate synthase G
3	View Details	[422..554]	PSI-BLAST	138.228787	Malate synthase G

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer	4.27762948199878	bayes_pls_golite062009
transferase activity, transferring acyl groups	2.61158006337608	bayes_pls_golite062009
catalytic activity	1.23634637968745	bayes_pls_golite062009
transferase activity	0.263512092168681	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2