Protein Overview: DFG5

General Information:
Name(s) found:	DFG5 / YMR238W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|887619\|emb\|CAA90209.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323894\|ref\|NP_013965.1\| Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p; Dfg5p [Saccharomyces cerevisi [NCBI NR] gi\|2497199\|sp\|Q05031.1\|DFG5_YEAST RecName: Full=Mannan endo-1,6-alpha-mannosidase DFG5; AltName: Full=Endo-alpha-1->6-D-mannanase DFG5; Flags: Precursor [NCBI NR] pir\|\|S57605 probable membrane protein YMR238w - yeast (Saccharomyces cerevisiae) [NCBI NR] Mannan endo-1,6-alpha-mannosidase DFG5 OS=Saccharomyces cerevisiae GN=DFG5 PE=1 SV=1 [Swiss-Prot] DFG5 Protein required for filamentous growth, cell polarity, and cellular elongation [mips] Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p [SGD] DFG5, Chr XIII from 746352-747728 [yeast_orfs2] Chr XIII from 746352-747728 [yeastorf3.fasta] (Q05031) Hypothetical 50.5 kDa protein in RNA1-RNT1 intergenic region [4932.FASTACCERV.txt] DFG5 SGDID:S0004851, Chr XIII from 746352-747728, Verified ORF [yeastflysequest.fasta] Dfg5p [Saccharomyces cerevisiae]gi\|887619\|emb\|CAA90209.1\| unknown [Saccharomyces cerevisiae]gi\|1362402\|pir\|\|S57605 probable membrane protein YMR238w - yeast (Saccharomyces cerevisiae)gi\|2497199\|sp\|Q05031\|DFG5_YEAST Mannan endo-1,6-alpha-mannosidase DFG [nrdb_11152004_Chlamydomonas] (Q05031) Mannan endo-1,6-alpha-mannosidase DFG5 precursor (EC 3.2.1.101) (Endo-alpha-1->6-D-mannanase DFG5) [cerv4932.FASTAC] DFG5 SGDID:S000004851, Chr XIII from 746352-747728, Verified ORF, "Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mannan endo-1,6-alpha-mannosidase DFG5 precursor (Endo-alpha-1->6-D-mannanase DFG5) [nr-271106-contam.fasta] Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p; Dfg5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DFG5 SGDID:S000004851, Chr XIII from 746352-747728, Verified ORF, "Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Mannan endo-1,6-alpha-mannosidase DFG5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DFG5 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DFG5 SGDID:S000004851, Chr XIII from 746353-747729, Genome Release 64-1-1, Verified ORF, "Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p" [yeast-030211-contam.fasta] DFG5 SGDID:S000004851, Chr XIII from 746353-747729, Genome Release 64-2-1, Verified ORF, "Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p" [20141208_orf_trans.fasta] TR6947\|c0_g1_i1\|g.4979 ORF TR6947\|c0_g1_i1\|g.4979 TR6947\|c0_g1_i1\|m.4979 type:complete len:459 (+) TR6947\|c0_g1_i1:3415-4791(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YMR238W SGDID:S000004851, Chr XIII from 746353-747729, Genome Release 64-1-1, Verified ORF, "Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_4244\|g.2437 ORF ID=asmbl_4244\|g.2437 ID=asmbl_4244\|m.2437 type:complete len:459 (+) ID=asmbl_4244:139-1515(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	458 amino acids
Gene Ontology:
Cellular Component:	anchored to plasma membrane [IDA]
Biological Process:	fungal-type cell wall biogenesis [IDA] pseudohyphal growth [IMP] budding cell bud growth [IGI]
Molecular Function:	mannosidase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIVNISAKMI LSICFTFLSF FKATHAMDLD TTSKTSICDA TALIQGGMLD YYEGTRYGGT  60
   61 VGMFQSPYYW WHAGEAFGGM LENWFLCEND TYQELLYDAL LAQTGSNYDY IPSNQTMVEG 120
  121 NDDQGIWGIT VMGAVERNFT DPGDGKPGWL AMVQAVFNTM YSRWDSEHCG GGLRWQIFTW 180
  181 NSGYNYKNTV SNACLFQIAA RLGRYTGNTT YLEVAEQVFD WLVDVGYVVL NDTANVFDGA 240
  241 EIDTNCTDIT KIEWTYNHGI VLGGLAYMYN ATNGTGEWET SLTKILNGAK SYFFKDSIMY 300
  301 ESACQDYGTC NTDQRTFKSI FSRMLGLTSV MAPFTRDTID DLIKTSAEAA AKSCNGGTDG 360
  361 HTCGLNWQKQ TNDGYYGLGE QMSALEVIQN LLIHDRPAPY KEDNGGTSKG DANAGMNSST 420
  421 TNVLQNNLNI KKGDRAGAAI ITAVILSVLT GGAVWMLF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		DCW1 DFG5 RHO1 YLR194C YPS3

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GIC2	DFG5	2	Unpublished Fields Lab Data
View Screen	GIC1	DFG5	2	Drees BL, et al. (2001)
View Screen	DCN1	DFG5	2	Unpublished Fields Lab Data
View Screen	VPS5	DFG5	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DFG5	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..458]	Pfam	259.080922	Glycosyl hydrolase family 76 No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
trehalase activity	4.23942428290504	bayes_pls_golite062009
hydrolase activity, acting on glycosyl bonds	3.78681366425382	bayes_pls_golite062009
alpha,alpha-trehalase activity	3.55712700159445	bayes_pls_golite062009
hydrolase activity, hydrolyzing O-glycosyl compounds	3.06084463737498	bayes_pls_golite062009
hydrolase activity	2.4324152075641	bayes_pls_golite062009
glucosidase activity	1.29725707798041	bayes_pls_golite062009
binding	1.09492132758288	bayes_pls_golite062009
catalytic activity	1.0882760267763	bayes_pls_golite062009
glucan 1,4-alpha-glucosidase activity	0.459801253504163	bayes_pls_golite062009
protein binding	0.208998323911823	bayes_pls_golite062009
intramolecular oxidoreductase activity	0.0402220178011703	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2