Protein Overview: GUA1

General Information:
Name(s) found:	GUA1 / YMR217W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854469\|emb\|CAA89932.1\| Gua1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323873\|ref\|NP_013944.1\| GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; Gua1p [NCBI NR] gi\|1708073\|sp\|P38625.4\|GUAA_YEAST RecName: Full=GMP synthase [glutamine-hydrolyzing]; AltName: Full=Glutamine amidotransferase; AltName: Full=GMP synthetase [NCBI NR] pir\|\|S55099 GMP synthase (glutamine-hydrolyzing) (EC 6.3.5.2) [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR] GMP synthase [glutamine-hydrolyzing] OS=Saccharomyces cerevisiae GN=GUA1 PE=1 SV=4 [Swiss-Prot] GUA1 GMP synthase (glutamine-hydrolyzing) [mips] GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation [SGD] GUA1, Chr XIII from 701789-703366 [yeast_orfs2] Chr XIII from 701789-703366 [yeastorf3.fasta] GUA1 SGDID:S0004830, Chr XIII from 701789-703366, Verified ORF [yeastflysequest.fasta] GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation [Saccharomyces cerevisiae]gi\|85446 [nrdb_11152004_Chlamydomonas] GUA1 SGDID:S000004830, Chr XIII from 701789-703366, Verified ORF, "GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively r [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Gua1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GMP synthase [glutamine-hydrolyzing] (Glutamine amidotransferase) (GMP synthetase) [nr-271106-contam.fasta] GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; Gua1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GUA1 SGDID:S000004830, Chr XIII from 701789-703366, Verified ORF, "GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] GMP synthase [glutamine-hydrolyzing] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GUA1 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GUA1 SGDID:S000004830, Chr XIII from 701790-703367, Genome Release 64-1-1, Verified ORF, "GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA" [yeast-030211-contam.fasta] GUA1 SGDID:S000004830, Chr XIII from 701790-703367, Genome Release 64-2-1, Verified ORF, "GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA" [20141208_orf_trans.fasta] TR4159\|c0_g1_i1\|g.2977 ORF TR4159\|c0_g1_i1\|g.2977 TR4159\|c0_g1_i1\|m.2977 type:complete len:526 (+) TR4159\|c0_g1_i1:62-1639(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR5523\|c0_g1_i1\|g.3944 ORF TR5523\|c0_g1_i1\|g.3944 TR5523\|c0_g1_i1\|m.3944 type:complete len:526 (+) TR5523\|c0_g1_i1:64-1641(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YMR217W SGDID:S000004830, Chr XIII from 701790-703367, Genome Release 64-1-1, Verified ORF, "GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4247\|g.3189 ORF asmbl_4247\|g.3189 asmbl_4247\|m.3189 type:complete len:526 (+) asmbl_4247:60-1637(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4256\|g.2458 ORF ID=asmbl_4256\|g.2458 ID=asmbl_4256\|m.2458 type:complete len:526 (+) ID=asmbl_4256:201-1778(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4252\|g.2395 ORF ID=asmbl_4252\|g.2395 ID=asmbl_4252\|m.2395 type:complete len:526 (+) ID=asmbl_4252:60-1637(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	525 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	GMP metabolic process [TAS]
Molecular Function:	GMP synthase (glutamine-hydrolyzing) activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL GWTPKGVILS  60
   61 GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING KQVGRGDKRE YGPATLKVID 120
  121 DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK TIATSDNSPY CGIVHETKPI YGIQFHPEVT 180
  181 HSTQGKTLLK NFAVDLCHAK QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS 240
  241 KLMTEAIGDR FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP 300
  301 EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG PSQTIKTHHN 360
  361 VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR HPFPGPGIAI RVLGEVTKEQ 420
  421 VEIARKADNI YIEEIKKAGL YNQISQAFAC LLPVKSVGVM GDQRTYDQVI ALRAIETTDF 480
  481 MTADWFPFEH SFLKKVASRI VNEVDGVARV TYDITSKPPA TVEWE

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	APC5	GUA1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..203]	PSI-BLAST	2510.0	GMP synthetase; GMP synthetase, central domain; GMP synthetase C-terminal dimerisation domain
2	View Details	[204..403]	PSI-BLAST	2510.0	GMP synthetase; GMP synthetase, central domain; GMP synthetase C-terminal dimerisation domain
3	View Details	[404..525]	PSI-BLAST	2510.0	GMP synthetase; GMP synthetase, central domain; GMP synthetase C-terminal dimerisation domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	2.70451256281397	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	2.33781404450996	bayes_pls_golite062009
phosphoribosylformylglycinamidine synthase activity	1.83575136996743	bayes_pls_golite062009
carbamoyl-phosphate synthase activity	1.74828881793189	bayes_pls_golite062009
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	1.74029540439985	bayes_pls_golite062009
ligase activity	1.70348758031146	bayes_pls_golite062009
catalytic activity	1.30391542469987	bayes_pls_golite062009
GMP synthase activity	0.413068109408727	bayes_pls_golite062009
anthranilate synthase activity	0.243205818526006	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2