Protein Overview: EFR3

General Information:
Name(s) found:	EFR3 / YMR212C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854464\|emb\|CAA89927.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323868\|ref\|NP_013939.1\| Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies and is phosphorylate [NCBI NR] gi\|2497190\|sp\|Q03653.1\|EFR3_YEAST RecName: Full=Protein EFR3 [NCBI NR] pir\|\|S55094 probable membrane protein YMR212c - yeast (Saccharomyces cerevisiae) [NCBI NR] (Q03653) Uncharacterized protein YMR212C [Swiss-Prot] Protein EFR3 OS=Saccharomyces cerevisiae GN=EFR3 PE=1 SV=1 [Swiss-Prot] EFR3 Protein required for cell viability [mips] Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies and is phosphorylated [SGD] EFR3, Chr XIII from 690694-693042, reverse complement [yeast_orfs2] Chr XIII from 690694-693042, reverse complement [yeastorf3.fasta] Chr XIII from 693042-690694, reverse complement [yeastorf5.fasta] (Q03653) Hypothetical 89.2 kDa protein in RAR1-SCJ1 intergenic region [4932.FASTACCERV.txt] EFR3 SGDID:S0004825, Chr XIII from 693042-690694, reverse complement, Verified ORF [yeastflysequest.fasta] Efr3p [Saccharomyces cerevisiae]gi\|854464\|emb\|CAA89927.1\| unknown [Saccharomyces cerevisiae]gi\|2497190\|sp\|Q03653\|YM62_YEAST Hypothetical 89.2 kDa protein in RAR1-SCJ1 intergenic regiongi\|1078543\|pir\|\|S55094 probable membrane protein YMR212c - yeast (Sa [nrdb_11152004_Chlamydomonas] EFR3 SGDID:S000004825, Chr XIII from 693042-690694, reverse complement, Verified ORF, "Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; green fluorescent protein (GFP)-fusion protein localizes to the ce [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] EFR3 SGDID:S000004825, Chr XIII from 693042-690694, reverse complement, Verified ORF, "Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; the authentic, non-tagged protein is detected in highly purified m [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; Efr3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized protein YMR212C [nr-271106-contam.fasta] EFR3 SGDID:S000004825, Chr XIII from 693042-690694, reverse complement, Verified ORF, "Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies and is phosphorylated" [yeast-bovrabprots.fasta] EFR3 SGDID:S000004825, Chr XIII from 693042-690694, reverse complement, Verified ORF, "Non-essential protein of unknown function; exhibits synthetic lethal genetic interactions with PHO85; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" [yeast-contam_custom.fasta] [chemostatdb.fasta] Protein EFR3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EFR3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] EFR3 SGDID:S000004825, Chr XIII from 693043-690695, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein required for Stt4-containing phosphoinositide kinase patch assembly at the plasma membrane; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene" [yeast-030211-contam.fasta] EFR3 SGDID:S000004825, Chr XIII from 693043-690695, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein required for Stt4-containing PI kinase complex localization; required for Stt4-containing phosphoinositide (PI) kinase patch assembly at the plasma membrane; recruited to the plasma membrane via a conserved basic patch near its N-terminus; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene" [20141208_orf_trans.fasta] YMR212C SGDID:S000004825, Chr XIII from 693043-690695, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein required for Stt4-containing phosphoinositide kinase patch assembly at the plasma membrane; exhibits synthetic lethal genetic interactions with PHO85; has sequence similarity to the Drosophila rolling blackout (RBO) gene" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	782 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ]
Biological Process:	cell morphogenesis [IGI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQLSMRMMFT PKHQKLVNQC YPTGRTTDKK PKSSETSYLL YYVNSRRSKL EKVSTYLIKR  60
   61 STSDLNHRRI GNIAVTLDLM NKIVLHCKEN LNVFVKDFLY IMNKVLSNNN FNNDVSVVEL 120
  121 IELAFSSICQ NLDDVLCNGD MEFVQLYQNF VDLFFKIVTE RIHNDDMLLK CCIDISNTNS 180
  181 VSSNPQLNHF VSKSVAYTIS KFQERNPKFK TLSLEAALES NLGKRLSRTQ TRTIGLDKAA 240
  241 EDNHDLSVKA LQSYFNTTET DKLNLSIRTL LRCLQSTPNK ELLEFVCNGI PVQLRYIVIL 300
  301 LLVRQLSDKD KNVNPIVSLK LMSSLLVSDV SIVGLSVLDI MRKLLNFQLK NATNKEVVAQ 360
  361 SCITMTDLNH KTYYAEQTSD MLYELLLKLK SDTVKDVEKN AVVEDIDFLV EHITQPSISL 420
  421 ELFIDLAHYM KNHIICLFNI VETEVPSSIL FSKLYSLLRE LDSHGVQKEM MEEIFDKYGK 480
  481 MALLSGLNYF LENVSEPEYT YYSYHLQAAN FLKLNDYKSQ TEYKMQTRTL FTKEDLLSYY 540
  541 SDTGSNKYSK KGAQILLSRD NQISTSDLLS DSQVRTTPLE YKNVPNAIFS NGKAVYDNND 600
  601 FAAKQNKFDN SIDDNIEEAN DTVISDANAK GSIYRFVAED ARSWKTMRAT APKVSDLKKT 660
  661 MNEKNIPNNM KRDGSFRGSQ SVKSRVTNIT FLLNELKTFS DDANKIKDPD EENIVGLDKI 720
  721 DVARSNSLRL APISSLSDRS SIGNRKSFLQ KTATGENQND DFKDANEDLH SLSSRGKIFS 780
  781 ST

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YPP1	EFR3	1	Unpublished Fields Lab Data
View Screen	YKU80	EFR3	1	Unpublished Fields Lab Data
View Screen	NAF1	EFR3	1	Hazbun TR, et al. (2003)
View Screen	PPG1	EFR3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	EFR3	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..416]	MSA	4.012968	View MSA. No confident structure predictions are available.
2	View Details	[417..782]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2