Protein Overview: URA4

General Information:
Name(s) found:	URA4 / YLR420W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|632670\|gb\|AAB67491.1\| Ura4p: dihydroorotase [Saccharomyces cerevisiae] [NCBI NR] gi\|6323452\|ref\|NP_013524.1\| Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate; Ura4p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|DEBYO dihydroorotase (EC 3.5.2.3) URA4 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1709957\|sp\|P20051.2\|PYRC_YEAST RecName: Full=Dihydroorotase; Short=DHOase [NCBI NR] Dihydroorotase OS=Saccharomyces cerevisiae GN=URA4 PE=1 SV=2 [Swiss-Prot] URA4 Dihydroorotase [mips] Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate [SGD] URA4, Chr XII from 963780-964874 [yeast_orfs2] Chr XII from 963780-964874 [yeastorf3.fasta] (P20051) Dihydroorotase (EC 3.5.2.3) (DHOase) [4932.FASTACCERV.txt] URA4 SGDID:S0004412, Chr XII from 963781-964875, Verified ORF [yeastflysequest.fasta] Third step in pyrimidine biosynthesis pathway; dihydrooratase [Saccharomyces cerevisiae]gi\|1709957\|sp\|P20051\|PYRC_YEAST Dihydroorotase (DHOase)gi\|2144514\|pir\|\|DEBYO dihydroorotase (EC 3.5.2.3) URA4 - yeast (Saccharomyces cerevisiae)gi\|632670\|gb\|AAB6749 [nrdb_11152004_Chlamydomonas] URA4 SGDID:S000004412, Chr XII from 963781-964875, Verified ORF, "Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] URA4 SGDID:S000004412, Chr XII from 963782-964876, Verified ORF, "Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate" [yeast-contam.fasta] Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate; Ura4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Dihydroorotase (DHOase) [nr-271106-contam.fasta] Ura4p: dihydroorotase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Dihydroorotase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=URA4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] URA4 SGDID:S000004412, Chr XII from 963785-964879, Genome Release 64-1-1, Verified ORF, "Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate" [yeast-030211-contam.fasta] URA4 SGDID:S000004412, Chr XII from 963785-964879, Genome Release 64-2-1, Verified ORF, "Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate" [20141208_orf_trans.fasta] YLR420W SGDID:S000004412, Chr XII from 963785-964879, Genome Release 64-1-1, Verified ORF, "Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	364 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	'de novo' pyrimidine base biosynthetic process [TAS] pyrimidine nucleotide biosynthetic process [IMP]
Molecular Function:	dihydroorotase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVQEIDLGLT CDMHVHVREG AMCELVTPKI RDGGVSIAYI MPNLQPPITT LDRVIEYKKT  60
   61 LQKLAPKTTF LMSFYLSKDL TPDLIHEAAQ QHAIRGVKCY PAGVTTNSAA GVDPNDFSAF 120
  121 YPIFKAMQEE NLVLNLHGEK PSVHDGDKEP IHVLNAEEAF LPALKKLHND FPNLKIILEH 180
  181 CTSESAIKTI EDINKNVKKA TDVKVAATLT AHHLFLTIDD WAGNPVNFCK PVAKLPNDKK 240
  241 ALVKAAVSGK PYFFFGSDSA PHPVQNKANY EGVCAGVYSQ SFAIPYIAQV FEEQNALENL 300
  301 KGFVSDFGIS FYEVKDSEVA SSDKAILFKK EQVIPQVISD GKDISIIPFK AGDKLSWSVR 360
  361 WEPR

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	URA4	1	Unpublished Fields Lab Data
View Screen	CUS1	URA4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	URA4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..364]	PSI-BLAST	444.54902	Dihydroorotase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
dihydroorotase activity	2.32070383330692	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides	2.20489529944693	bayes_pls_golite062009
deaminase activity	1.79186203771221	bayes_pls_golite062009
catalytic activity	1.70199291046899	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1.60895003474033	bayes_pls_golite062009
nucleic acid binding	0.629962267754185	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	0.573107361273483	bayes_pls_golite062009
transcription regulator activity	0.506159010274668	bayes_pls_golite062009
DNA binding	0.462348875991261	bayes_pls_golite062009
binding	0.459041534336076	bayes_pls_golite062009
hydrolase activity	0.24275130580417	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2