Protein Overview: NNT1

General Information:
Name(s) found:	NNT1 / YLR285W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Putative nicotinamide N-methyltransferase, has a role in rDNA silencing and in lifespan determination [SGD]
Organism:	Saccharomyces cerevisiae
Length:	261 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	nicotinamide metabolic process [ISS] chromatin silencing at rDNA [IMP]
Molecular Function:	nicotinamide N-methyltransferase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDIESLGEA AGLFEEPEDF LPPPPKPHFA EYQRSHITKE SKSDVKDIKL RLVGTSPLWG  60
   61 HLLWNAGIYT ANHLDSHPEL IKGKTVLELG AAAALPSVIC ALNGAQMVVS TDYPDPDLMQ 120
  121 NIDYNIKSNV PEDFNNVSTE GYIWGNDYSP LLAHIEKIGN NNGKFDLIIL SDLVFNHTEH 180
  181 HKLLQTTKDL LAEKGQALVV FSPHRPKLLE KDLEFFELAK NEFHLVPQLI EMVNWKPMFD 240
  241 EDEETIEVRS RVYAYYLTHE K

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HXK2	NNT1	1	Unpublished Fields Lab Data
View Screen	MET18	NNT1	1	Unpublished Fields Lab Data
View Screen	SPT21	NNT1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NNT1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..261]	PSI-BLAST	25.39794	Arginine methyltransferase, HMT1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
DNA-methyltransferase activity	1.4114792557994	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	1.07629237539972	bayes_pls_golite062009
transferase activity	1.07321114919891	bayes_pls_golite062009
methyltransferase activity	1.06467956103007	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	1.04881630872808	bayes_pls_golite062009
transcription regulator activity	0.978540537169703	bayes_pls_golite062009
DNA binding	0.905031018963259	bayes_pls_golite062009
nucleic acid binding	0.848300230574943	bayes_pls_golite062009
histone methyltransferase activity	0.718593815666209	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	0.488694756043017	bayes_pls_golite062009
RNA methyltransferase activity	0.473668177706488	bayes_pls_golite062009
N-methyltransferase activity	0.344664739778274	bayes_pls_golite062009
binding	0.336188540273603	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.92

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2