Protein Overview: DBR1

General Information:
Name(s) found:	DBR1 / YKL149C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322700\|ref\|NP_012773.1\| RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition; Dbr1p [Saccharomyces cerevisiae] [NCBI NR] gi\|486256\|emb\|CAA81990.1\| DBR1 [Saccharomyces cerevisiae] [NCBI NR] gi\|407498\|emb\|CAA81504.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|172552\|gb\|AAA35023.1\| putative debranching enzyme [Saccharomyces cerevisiae] [NCBI NR] gi\|171382\|gb\|AAA34560.1\| debranching enzyme [NCBI NR] gi\|1582555\|prf\|\|2118404R ORF [NCBI NR] gi\|118288\|sp\|P24309.1\|DBR1_YEAST RecName: Full=Lariat debranching enzyme [NCBI NR] pir\|\|A38491 lariat-debranching enzyme - yeast (Saccharomyces cerevisiae) [NCBI NR] Lariat debranching enzyme OS=Saccharomyces cerevisiae GN=DBR1 PE=1 SV=1 [Swiss-Prot] DBR1 Lariat-debranching enzyme [mips] RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition [SGD] DBR1, Chr XI from 167614-168831, reverse complement [yeast_orfs2] Chr XI from 167614-168831, reverse complement [yeastorf3.fasta] Chr XI from 167613-168830, reverse complement [yeastorf4.fasta] Chr XI from 168830-167613, reverse complement [yeastorf5.fasta] (P24309) Lariat debranching enzyme (EC 3.1.-.-) [4932.FASTACCERV.txt] DBR1 SGDID:S0001632, Chr XI from 168834-167617, reverse complement, Verified ORF [yeastflysequest.fasta] Dbr1p [Saccharomyces cerevisiae]gi\|407498\|emb\|CAA81504.1\| unknown [Saccharomyces cerevisiae]gi\|486256\|emb\|CAA81990.1\| DBR1 [Saccharomyces cerevisiae]gi\|101276\|pir\|\|A38491 lariat-debranching enzyme - yeast (Saccharomyces cerevisiae)gi\|118288\|sp\|P24309\| [nrdb_11152004_Chlamydomonas] DBR1 SGDID:S000001632, Chr XI from 168834-167617, reverse complement, Verified ORF, "RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DBR1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition; Dbr1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORF [nr-271106-contam.fasta] debranching enzyme [nr-271106-contam.fasta] Lariat debranching enzyme [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Lariat debranching enzyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DBR1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DBR1 SGDID:S000001632, Chr XI from 168829-167612, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition" [yeast-030211-contam.fasta] DBR1 SGDID:S000001632, Chr XI from 168829-167612, Genome Release 64-2-1, reverse complement, Verified ORF, "RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies" [20141208_orf_trans.fasta] YKL149C SGDID:S000001632, Chr XI from 168829-167612, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	405 amino acids
Gene Ontology:
Cellular Component:	nucleus [IC]
Biological Process:	transposition, RNA-mediated [IMP] snoRNA metabolic process [IMP] pseudohyphal growth [IMP] RNA catabolic process [IDA][IMP]
Molecular Function:	RNA lariat debranching enzyme activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTKLRIAVQG CCHGQLNQIY KEVSRIHAKT PIDLLIILGD FQSIRDGQDF KSIAIPPKYQ  60
   61 RLGDFISYYN NEIEAPVPTI FIGGNHESMR HLMLLPHGGY VAKNIFYMGY SNVIWFKGIR 120
  121 IGSLSGIWKE WDFNKQRPDW NDLENNNWKA NIRNLYHVRI SDIAPLFMIK HRIDIMLSHD 180
  181 WPNGVVYHGD TKHLLKLKPF FEQDIKEGKL GSPVTWQLLR DLRPQWWLSA HLHVRFMASI 240
  241 KHNKRSHEPP NKSTSKTKKN NNEIDLDLSS DEDERSGIMN CQEENEYDSK YGETRFLALD 300
  301 KCLPRRRWLE ILEIEPDTSH ASWKDENHRM FWDPEFINNL VICQKNKNLL SNKPFNSVNW 360
  361 IELSQSNREE GRDIDWENYA IPAYTLDIQK DEVRQTKAFI SKFMT

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPT23	DBR1	1	Unpublished Fields Lab Data
View Screen	COG4	DBR1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	DBR1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..260]	ORFEUS	7.05	Purple acid phosphatase, N-terminal domain; Plant purple acid phosphatase, catalytic domain
2	View Details	[261..405]	MSA	1.007986	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
hydrolase activity	2.53080794424314	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	2.41348361064083	bayes_pls_golite062009
binding	1.30309680165893	bayes_pls_golite062009
catalytic activity	1.30037658165549	bayes_pls_golite062009
phosphoprotein phosphatase activity	0.768201004870964	bayes_pls_golite062009
protein serine/threonine phosphatase activity	0.634242884531037	bayes_pls_golite062009
phosphoric ester hydrolase activity	0.463083693607652	bayes_pls_golite062009
phosphatase activity	0.325160962990544	bayes_pls_golite062009
protein binding	0.20380491058914	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2