Protein Overview: EPS1

General Information:
Name(s) found:	EPS1 / YIL005W [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family [SGD]
Organism:	Saccharomyces cerevisiae
Length:	701 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum membrane [IDA]
Biological Process:	protein retention in ER lumen [IMP]
Molecular Function:	protein disulfide isomerase activity [IMP][ISS] protein disulfide oxidoreductase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKMNLKRLVV TFFSCITFLL KFTIAAAEPP EGFPEPLNPT NFKEELSKGL HIIDFYSPYC  60
   61 PHCKHLAPVW METWEEFKEE SKTLNITFSQ VNCIESADLC GDENIEYFPE IRLYNPSGYI 120
  121 KSFTETPRTK ESLIAFARRE SMDPNNLDTD LDSAKSESQY LEGFDFLELI AGKATRPHLV 180
  181 SFWPTKDMKN SDDSLEFKNC DKCHEFQRTW KIISRQLAVD DINTGHVNCE SNPTICEELG 240
  241 FGDLVKITNH RADREPKVAL VLPNKTSNNL FDYPNGYSAK SDGYVDFARR TFTNSKFPNI 300
  301 TEGELEKKAN RDIDFLQERG RVTNNDIHLV FSYDPETVVI EDFDILEYLI EPLSKIPNIY 360
  361 LHQIDKNLIN LSRNLFGRMY EKINYDASQT QKVFNKEYFT MNTVTQLPTF FMFKDGDPIS 420
  421 YVFPGYSTTE MRNIDAIMDW VKKYSNPLVT EVDSSNLKKL ISFQTKSYSD LAIQLISSTD 480
  481 HKHIKGSNKL IKNLLLASWE YEHIRMENNF EEINERRARK ADGIKKIKEK KAPANKIVDK 540
  541 MREEIPHMDQ KKLLLGYLDI SKEKNFFRKY GITGEYKIGD VIIIDKSNNY YYNKDNFGNS 600
  601 LTSNNPQLLR EAFVSLNIPS KALYSSKLKG RLINSPFHNV LSFLDIIHGN GMPGYLIVIV 660
  661 LFIAILKGPS IYRRYKVRKH YRAKRNAVGI LGNMEKKKNQ D

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ASF1	3829 - low filter	Green EM, et al (2005)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CAB3	EPS1	2	Hazbun TR, et al. (2003)
View Screen	FRT2	EPS1	1	Unpublished Fields Lab Data
View Screen	SDP1	EPS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	EPS1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..165]	PSI-BLAST	86.274545	Protein disulfide isomerase, PDI
2	View Details	[166..400]	PSI-BLAST	4.30103	Calsequestrin
3	View Details	[401..597]	PSI-BLAST	5.522879	Membrane-anchored thioredoxin-like protein TlpA, soluble domain
4	View Details	[598..701]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2