Protein Overview: DUO1

General Information:
Name(s) found:	DUO1 / YGL061C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321377\|ref\|NP_011454.1\| Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Duo1p [Sa [NCBI NR] gi\|45270858\|gb\|AAS56810.1\| YGL061C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S64065 hypothetical protein YGL061c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1723843\|sp\|P53168.1\|DUO1_YEAST RecName: Full=DASH complex subunit DUO1; AltName: Full=Outer kinetochore protein DUO1; AltName: Full=Death upon overproduction protein 1 [NCBI NR] gi\|1322564\|emb\|CAA96764.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P53168) Hypothetical 27.5 kDa protein in PYC1-UBC2 intergenic region [Swiss-Prot] DASH complex subunit DUO1 OS=Saccharomyces cerevisiae GN=DUO1 PE=1 SV=1 [Swiss-Prot] DUO1 Outer kinetochore protein - part of Dam1 complex [mips] Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis [SGD] DUO1, Chr VII from 388966-389709, reverse complement [yeast_orfs2] Chr VII from 388966-389709, reverse complement [yeastorf3.fasta] Chr VII from 388969-389712, reverse complement [yeastorf4.fasta] Chr VII from 389712-388969, reverse complement [yeastorf5.fasta] DUO1 SGDID:S0003029, Chr VII from 389712-388969, reverse complement, Verified ORF [yeastflysequest.fasta] DUO1 SGDID:S0003029, Chr VII from 389714-388971, reverse complement, Verified ORF [10112005cervSGD.fasta] Duo1p [Saccharomyces cerevisiae]gi\|1322564\|emb\|CAA96764.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|45270858\|gb\|AAS56810.1\| YGL061C [Saccharomyces cerevisiae]gi\|2131574\|pir\|\|S64065 hypothetical protein YGL061c - yeast (Saccharomyces cerevis [nrdb_11152004_Chlamydomonas] DUO1 SGDID:S000003029, Chr VII from 389714-388971, reverse complement, Verified ORF, "Essential mitotic spindle protein required to maintain spindle integrity and kinetochore function, part of the multisubunit DASH complex which binds microtubules and is [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DUO1 SGDID:S000003029, Chr VII from 389714-388971, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is [yeast-contam.fasta] DASH complex subunit DUO1 (Outer kinetochore protein DUO1) (Death upon overproduction protein 1) [nr-271106-contam.fasta] Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis; Duo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YGL061C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DUO1 SGDID:S000003029, Chr VII from 389714-388971, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DASH complex subunit DUO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DUO1 SGDID:S000003029, Chr VII from 389711-388968, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis" [yeast-030211-contam.fasta] DUO1 SGDID:S000003029, Chr VII from 389711-388968, Genome Release 64-2-1, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis" [20141208_orf_trans.fasta] YGL061C SGDID:S000003029, Chr VII from 389711-388968, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	247 amino acids
Gene Ontology:
Cellular Component:	spindle microtubule [IDA] DASH complex [IDA ][IPI ] spindle pole body [IDA] spindle [IPI] condensed nuclear chromosome kinetochore [IPI]
Biological Process:	mitotic spindle organization in nucleus [IPI] regulation of microtubule polymerization or depolymerization [IDA][IPI]
Molecular Function:	structural constituent of cytoskeleton [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEQSQLDDS TIDKLIPQIF NEMRSNLNNT TNKFPKSTGG GASDNISANS NSIRSFNSIT  60
   61 TQSLLKESES LDKITAMIKN VTAALKNNLP VYVNQVHEVC KSTNSILDSW INIHSQAGYI 120
  121 HKLMSDQTYL KLINDRLHNE NVNTNDEDGS TLHNVIALKK KEILDLRQKL ENRKGEKDAA 180
  181 PAKPPNQGLN PRYGVQSGRR PVPSAGISNN GRVRKTHVPA SKRPSGIPRV TNRWTKPTAS 240
  241 SSRKMFR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ASK1 DAD1 DAD2 DAD3 DAM1 DUO1 HSK3 SPC19 SPC34
View Details	(MIPS) Mewes HW, et al. (2004)		ASK1 DAD1 DAD2 DAD3 DAD4 DAM1 DUO1 SPC19 SPC34
View Details	Krogan NJ, et al. (2006)		ASK1 DAD1 DAD4 DAM1 DUO1 HSK3 NCA2 SPC19 SPC34
View Details	Qiu et al. (2008)		ARP10 ASK1 BBP1 DAD1 DAD2 DAD3 DAD4 DAM1 DUO1 HSK3 NUF2 SPC19 SPC25 SPC34 SPC42

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	No Comments	Cheeseman IM, et al. (2002)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	DUO1 and SPC42	Huh WK, et al. (2003)
View Data	DUO1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..247]	deduced	N/A	No confident structure predictions are available.
2	View Details	[175..247]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: DUO1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: