Protein Overview: GYP8

General Information:
Name(s) found:	GYP8 / YFL027C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836727\|dbj\|BAA09211.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|51013725\|gb\|AAT93156.1\| YFL027C [Saccharomyces cerevisiae] [NCBI NR] gi\|16740526\|ref\|NP_444296.1\| GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport; Gyp8p [Saccharomyces cer [NCBI NR] gi\|1175935\|sp\|P43570.1\|GYP8_YEAST RecName: Full=GTPase-activating protein GYP8 [NCBI NR] pir\|\|S56227 probable membrane protein YFL027c - yeast (Saccharomyces cerevisiae) [NCBI NR] GTPase-activating protein GYP8 OS=Saccharomyces cerevisiae GN=GYP8 PE=1 SV=1 [Swiss-Prot] GYP8 GTPase-activating protein for yeast Rab family members, involved in the regulation of ER to Golgi vesicle transport [mips] GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport [SGD] YFL027C, Chr VI from 80417-81910, reverse complement [yeast_orfs2] Chr VI from 80417-81910, reverse complement [yeastorf3.fasta] Chr VI from 81910-80417, reverse complement [yeastorf5.fasta] (P43570) GTPase-activating protein GYP8 [4932.FASTACCERV.txt] GYP8 SGDID:S0001867, Chr VI from 81910-80417, reverse complement, Verified ORF [yeastflysequest.fasta] GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport [Saccharomyces cerevisiae]gi\|51013725\|gb\|AAT93156.1\| [nrdb_11152004_Chlamydomonas] GYP8 SGDID:S000001867, Chr VI from 81910-80417, reverse complement, Verified ORF, "GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of E [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YFL027C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport; Gyp8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTPase-activating protein GYP8 [nr-271106-contam.fasta] GYP8 SGDID:S000001867, Chr VI from 81910-80417, reverse complement, Verified ORF, "GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport" [yeast-bovrabprots.fasta] [chemostatdb.fasta] GTPase-activating protein GYP8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GYP8 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GYP8 SGDID:S000001867, Chr VI from 81912-80419, Genome Release 64-1-1, reverse complement, Verified ORF, "GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport" [yeast-030211-contam.fasta] GYP8 SGDID:S000001867, Chr VI from 81912-80419, Genome Release 64-2-1, reverse complement, Verified ORF, "GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport" [20141208_orf_trans.fasta] YFL027C SGDID:S000001867, Chr VI from 81912-80419, Genome Release 64-1-1, reverse complement, Verified ORF, "GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	497 amino acids
Gene Ontology:
Cellular Component:	intracellular [IDA]
Biological Process:	vesicle-mediated transport [IGI]
Molecular Function:	Rab GTPase activator activity [IGI][IPI][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPLRSLFHTN QSSHDKDALT RGGYNAYLES LSRCDSGKAE EQKGKVISKL LEKKDVRALR  60
   61 YIGLGPLGFV NNSLRKDCWY ELLASQLLID DATEYITPVE KHKDEGQVIL DAERSFGGIV 120
  121 DKNLKLQLRK LLVELITRVL RKYPTLNYYQ GYHDIVSVFI MCFSWNVTKE NGLELENLSL 180
  181 QEEIDMERLF YCIEAFTLLY LRDFMMNSLD FSFEQLRVIS SLIKESNMKF YNLFKFDENE 240
  241 PLFAIGSILT IFAHNLKPID SGDNNLHKIL FQIFDMTISM QSMRLPLIIY KNLLLQNASE 300
  301 ISKQIEANSD FFENDFDLRH GAIQTVLQKK LYDEALWEEV LQITRKDATT ASKKALKRVS 360
  361 LNKYSALLNT ACGKPGCFDM STIIFYLSEQ TKMNEHYKEE KYHGVAARSK TRALVQRLGH 420
  421 FLPSKYNRWS KISLLIGIVA ILYQLRTTRS LSLVLNLRYM ISTKLKDLSH ININLHHVSH 480
  481 IWVDPIRDIL KLGHPTR

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	GYP8	1	Unpublished Fields Lab Data
View Screen	SAM35	GYP8	1	Unpublished Fields Lab Data
View Screen	RCY1	GYP8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GYP8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..210] [272..311]	PSI-BLAST	37.0	Ypt/Rab-GAP domain of gyp1p
2	View Details	[211..271]	PSI-BLAST	37.0	Ypt/Rab-GAP domain of gyp1p
3	View Details	[312..497]	MSA	1.148993	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.76

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2