Protein Overview: BUD16

General Information:
Name(s) found:	BUD16 / YEL029C [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) [SGD]
Organism:	Saccharomyces cerevisiae
Length:	312 amino acids
Gene Ontology:
Cellular Component:	cellular_component [ND]
Biological Process:	cellular bud site selection [IMP] telomere maintenance [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPRLLATQSH VVHGYVGNKA ATFPLQCLGW DVDCCNSVQF SNHTGYGLDK VFGTITRETD  60
   61 LKELLSGLFD NFSQDYQALL SGYLPNKNSV RCMGTYYAKF KEANPEMIWL MDPVMGDEGQ 120
  121 LYVSEDVIPE YRKLALSPKQ LVDIITPNQF ELEILYGGEI KTKEHLKKAL KKLHQTIPVI 180
  181 IVTSCDCKMF DDKDFIYCVA SMEGKTPIVY RVPFIDSYFT GVGDLFSALL LDRVYKILSN 240
  241 PTTTLKFEDQ VNNVLNVIQK VLKITRSYAS GKMKAKMGSA LEMKEMELRL IESRDIYETI 300
  301 NIHQTDYIYA RL

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC7	BUD16	1	Unpublished Fields Lab Data
View Screen	CUS1	BUD16	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUD16	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..312]	PSI-BLAST	46.09691	4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (HMP-phosphate kinase, ThiD)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
kinase activity	3.96411195020495	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	3.67035256855428	bayes_pls_golite062009
transferase activity	3.30865420017814	bayes_pls_golite062009
lithium ion binding	3.00550221878163	bayes_pls_golite062009
sodium ion binding	2.93046843966773	bayes_pls_golite062009
pyridoxal kinase activity	2.62798458074822	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	2.56927916030784	bayes_pls_golite062009
phosphomethylpyrimidine kinase activity	2.12104666683074	bayes_pls_golite062009
pyridoxal binding	2.04719269680858	bayes_pls_golite062009
catalytic activity	1.74474587246986	bayes_pls_golite062009
binding	1.4216897826135	bayes_pls_golite062009
carbohydrate kinase activity	1.32862180479506	bayes_pls_golite062009
nucleic acid binding	1.23363144435362	bayes_pls_golite062009
transcription regulator activity	1.21529398967164	bayes_pls_golite062009
potassium ion binding	1.18018952731159	bayes_pls_golite062009
alkali metal ion binding	1.18018952731159	bayes_pls_golite062009
DNA binding	0.954497955488766	bayes_pls_golite062009
transcription factor activity	0.376784274163604	bayes_pls_golite062009
protein binding	0.117280046908929	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2