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Protein Overview: FLO1

General Information:
Name(s) found: FLO1 / YAR050W [SGD]
Description(s) found:
Found 24 descriptions.
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  • Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1537 amino acids

    Gene Ontology:
    Cellular Component: fungal-type cell wall [IDALink to PubMed articleLink to PubMed article]
    Biological Process: flocculation via cell wall protein-carbohydrate interaction [IMPLink to PubMed article]
    Molecular Function: mannose binding [IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTMPHRYMFL AVFTLLALTS VASGATEACL PAGQRKSGMN INFYQYSLKD SSTYSNAAYM  60
   61 AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED SYGNWGCKGM GACSNSQGIA 120
  121 YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG SYTFKFATVD DSAILSVGGA TAFNCCAQQQ 180
  181 PPITSTNFTI DGIKPWGGSL PPNIEGTVYM YAGYYYPMKV VYSNAVSWGT LPISVTLPDG 240
  241 TTVSDDFEGY VYSFDDDLSQ SNCTVPDPSN YAVSTTTTTT EPWTGTFTST STEMTTVTGT 300
  301 NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT DETIIVIRTP 360
  361 TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND 420
  421 TFTSTSTELT TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STELTTVTGT 480
  481 NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP 540
  541 TTATTAMTTP QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 600
  601 TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST STEMTTVTGT 660
  661 NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTTGLPT DETIIVIRTP 720
  721 TTATTAMTTT QPWNDTFTST STEMTTVTGT NGVPTDETVI VIRTPTSEGL ISTTTEPWTG 780
  781 TFTSTSTEMT TVTGTNGQPT DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT 840
  841 NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TITGTNGQPT DETVIVIRTP 900
  901 TSEGLISTTT EPWTGTFTST STEMTHVTGT NGVPTDETVI VIRTPTSEGL ISTTTEPWTG 960
  961 TFTSTSTEVT TITGTNGQPT DETVIVIRTP TSEGLISTTT EPWTGTFTST STEMTTVTGT1020
 1021 NGQPTDETVI VIRTPTSEGL VTTTTEPWTG TFTSTSTEMS TVTGTNGLPT DETVIVVKTP1080
 1081 TTAISSSLSS SSSGQITSSI TSSRPIITPF YPSNGTSVIS SSVISSSVTS SLFTSSPVIS1140
 1141 SSVISSSTTT STSIFSESSK SSVIPTSSST SGSSESETSS AGSVSSSSFI SSESSKSPTY1200
 1201 SSSSLPLVTS ATTSQETASS LPPATTTKTS EQTTLVTVTS CESHVCTESI SPAIVSTATV1260
 1261 TVSGVTTEYT TWCPISTTET TKQTKGTTEQ TTETTKQTTV VTISSCESDV CSKTASPAIV1320
 1321 STSTATINGV TTEYTTWCPI STTESRQQTT LVTVTSCESG VCSETASPAI VSTATATVND1380
 1381 VVTVYPTWRP QTANEESVSS KMNSATGETT TNTLAAETTT NTVAAETITN TGAAETKTVV1440
 1441 TSSLSRSNHA ETQTASATDV IGHSSSVVSV SETGNTKSLT SSGLSTMSQQ PRSTPASSMV1500
 1501 GYSTASLEIS TYAGSANSLL AGSGLSVFIA SLLLAII

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Qiu et al. (2008) FLO1 FLO5 FLO9

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..259] MSA 1.089987 View MSA. No confident structure predictions are available.
    2 View Details [260..316] Pfam 22.148742 Flocculin repeat No confident structure predictions are available.
    3 View Details [317..361] Pfam 22.886057 Flocculin repeat No confident structure predictions are available.
    4 View Details [362..406] Pfam 22.167491 Flocculin repeat No confident structure predictions are available.
    5 View Details [407..451] Pfam 22.657577 Flocculin repeat No confident structure predictions are available.
    6 View Details [452..496] Pfam 22.657577 Flocculin repeat No confident structure predictions are available.
    7 View Details [497..541] Pfam 22.236572 Flocculin repeat No confident structure predictions are available.
    8 View Details [542..586] Pfam 22.229148 Flocculin repeat No confident structure predictions are available.
    9 View Details [587..631] Pfam 22.508638 Flocculin repeat No confident structure predictions are available.
    10 View Details [632..676] Pfam 22.677781 Flocculin repeat No confident structure predictions are available.
    11 View Details [677..721] Pfam 20.337242 Flocculin repeat No confident structure predictions are available.
    12 View Details [722..766] Pfam 22.958607 Flocculin repeat No confident structure predictions are available.
    13 View Details [767..811] Pfam 23.769551 Flocculin repeat No confident structure predictions are available.
    14 View Details [812..856] Pfam 22.886057 Flocculin repeat No confident structure predictions are available.
    15 View Details [857..901] Pfam 23.60206 Flocculin repeat No confident structure predictions are available.
    16 View Details [902..946] Pfam 23.013228 Flocculin repeat No confident structure predictions are available.
    17 View Details [947..991] Pfam 22.920819 Flocculin repeat No confident structure predictions are available.
    18 View Details [992..1036] Pfam 23.769551 Flocculin repeat No confident structure predictions are available.
    19 View Details [1037..1094] Pfam 21.958607 Flocculin repeat No confident structure predictions are available.
    20 View Details [1095..1184] deduced N/A No confident structure predictions are available.
    21 View Details [1185..1537] MSA 7.163996 View MSA. No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
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