Protein Overview: AI4

General Information:
Name(s) found:	AI4 / Q0065 [SGD]
Description(s) found: Found 10 descriptions. SHOW ALL	Endonuclease I-SceII, encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity [SGD]
Organism:	Saccharomyces cerevisiae
Length:	544 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [TAS]
Biological Process:	RNA splicing [IDA] intron homing [IMP]
Molecular Function:	endonuclease activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG NSQLFNVLVM  60
   61 PALIGGFGNY LLPLMIGATD TAFPRINNIA FWVLPMGLVC LVTSTLVESG AGTGWTVYPP 120
  121 LSSIQAHSGP SVDLAIFALH LTSISSLLGA INFIVTTLNM RTNGMTMHKL PLFVWSIFIT 180
  181 AFLLLLSLPV LSAGITMLLL DRNFNTSFFE VSGGGDPILY EHLFWFFGQT VATIIMLMMY 240
  241 NDMHFSKCWK LLKKWITNIM STLFKALFVK MFMSYNNQQD KMMNNTMLKK DNIKRSSETT 300
  301 RKMLNNSMNK KFNQWLAGLI DGDGYFGIVS KKYVSLEITV ALEDEMALKE IQNKFGGSIK 360
  361 LRSGVKAIRY RLTNKTGMIK LINAVNGNIR NTKRLVQFNK VCILLGIDFI YPIKLTKDNS 420
  421 WFVGFFDADG TINYSFKNNH PQLTISVTNK YLQDVQEYKN ILGGNIYFDK SQNGYYKWSI 480
  481 QSKDMVLNFI NDYIKMNPSR TTKMNKLYLS KEFYNLKELK AYNKSSDSMQ YKAWLNFENK 540
  541 WKNK

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..425]	PSI-BLAST	135.0	Mitochondrial cytochrome c oxidase, subunit I
2	View Details	[426..544]	PSI-BLAST	9.221849	The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
hydrogen ion transmembrane transporter activity	2.35288611494101	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.17205034949175	bayes_pls_golite062009
heme-copper terminal oxidase activity	2.00288767149295	bayes_pls_golite062009
catalytic activity	1.95966890211023	bayes_pls_golite062009
cytochrome-c oxidase activity	1.60542104698557	bayes_pls_golite062009
oxidoreductase activity, acting on heme group of donors	1.60542104698557	bayes_pls_golite062009
oxidoreductase activity, acting on heme group of donors, oxygen as acceptor	1.60542104698557	bayes_pls_golite062009
binding	0.733970829597091	bayes_pls_golite062009
oxidoreductase activity	0.5109577542055	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.423208299895845	bayes_pls_golite062009
protein binding	0.358081002328109	bayes_pls_golite062009

2

Term	Confidence	Notes
nuclease activity	1.30590086180382	bayes_pls_golite062009
catalytic activity	1.00294942392567	bayes_pls_golite062009
binding	0.947409481120466	bayes_pls_golite062009
nucleic acid binding	0.92351380308588	bayes_pls_golite062009
hydrolase activity	0.908706495902542	bayes_pls_golite062009
endonuclease activity	0.892525491806151	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	0.861094108940112	bayes_pls_golite062009
DNA binding	0.571084560404215	bayes_pls_golite062009
RNA binding	0.407564756021673	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

Philius confidence legend

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