YRC Public Data Repository - Protein Structure Prediction

Protein:	YPL141C
Organism:	Saccharomyces cerevisiae
Length:	865 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for YPL141C.

Description	E-value	Query Range	Subject Range
gi\|151942658 - gi\|151942658\|gb\|EDN61004.1\| conserved protein [Saccharomyces cerevisiae YJM789] YPL141C - Putative protein kinase; similar to Kin4p; green fluorescent protein (GFP)-fusion protein localizes ... FRK1_YEAST - Fatty acyl-CoA synthetase and RNA processing-associated kinase 1 OS=Saccharomyces cerevisiae (strain...	0.0	[1..865]	[1..865]
MARK1 - MAP/microtubule affinity-regulating kinase 1	0.0	[1..765]	[7..763]
gi\|27694575 - gi\|27694575\|gb\|AAH43730.1\| Mark2-prov protein [Xenopus laevis]	0.0	[2..765]	[18..744]
MARK1_MOUSE - Serine/threonine-protein kinase MARK1 OS=Mus musculus GN=Mark1 PE=1 SV=1	0.0	[1..765]	[7..763]
MARK1_RAT - Serine/threonine-protein kinase MARK1 OS=Rattus norvegicus GN=Mark1 PE=1 SV=1	0.0	[1..765]	[7..761]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[28..765]	[7..713]

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Predicted Domain #1

Region A:
Residues: [1-99]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSYTNKRHTY YGGFTNDLSD TFQYPQRTDE QRRKHVTFGP YILGSTLGEG EFGKVKLGWP  60
   61 KNFSNSSNST FDFPKQVAIK LIKRDSISND YRKEVKIYR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [116-130]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EEVLQNSRYI GIVLE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	450.264245
Match:	1ia8A_
Description:	Cell cycle checkpoint kinase chk1
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [100-115]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EINALKHLSH PNIVKL

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [131-140]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YACGGEFYKY

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [156-205]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FSQLISGVHY IHSKGLVHRD LKLENLLLDK NENLVITDFG FVNEFCSRNE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	450.264245
Match:	1ia8A_
Description:	Cell cycle checkpoint kinase chk1
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [141-155]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IQKKRRLKEM NACRL

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [206-370]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LMKTSCGSPC YAAPELVISA EPYEARKADI WSCGVILYAI LAGYLPWDDD PNNPEGSDIG  60
   61 RLYNYINSTP LKFPDYILPI PRDLLRRMLV SDPKKRINLK QIKKHEWLKP HSSFLSITPD 120
  121 EWDKLNNTQS VFRLAKPRRR YGSRPQSSCS TSSLGSRSDK RDSLV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	450.264245
Match:	1ia8A_
Description:	Cell cycle checkpoint kinase chk1
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [371-865]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IDSTLITFPA PPQESQNHII TRPASIASDQ RLSPIRRSNR HNRSNSAASV ALQAVVNADR  60
   61 EYVLSHEQSL SPVQNIRQTT GNMTASLSPP PAISPGDIII ETTPIKRNTI SGSSIVPSLE 120
  121 EESSTTMQTS KIQPNNMASS QNHQYNKNKT QNSLQSAKNF YRTSSSSHTK PRPTSYHPGS 180
  181 YTTPPYNSNT LSIYEINEKA KSSASSQTLN QRDTSPFDST PYLALDTCIT SSSSIESSPK 240
  241 LITHGQFSVA KPSVDLQSVS GDLIKYKRDA DVVTRIYDEK YKQKRKSLRY SGIFSDISCD 300
  301 TVTEESDELR PPESPLQQHE GQESIDKAKT EDTSEKGSKS SNIAKATAQK HVNNHLERSL 360
  361 NEAESTKKRF SFLSLYSYDT SKSSLYSSMD SKRKPSPPSQ RRPKKDDSYQ TNSKNHYITA 420
  421 SNMQTSHQVS KDLPAPTMVQ NKCTLETKKA VRSNRSSIMV SEVNKASVDN KAAQSPEHST 480
  481 AKRVLGFFKR RSMKI

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
protein kinase activity	4.05475891009228	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	4.04751809021925	bayes_pls_golite062009
kinase activity	3.88704262604357	bayes_pls_golite062009
protein serine/threonine kinase activity	3.7974128326421	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	3.55786569314106	bayes_pls_golite062009
transferase activity	2.750643926194	bayes_pls_golite062009
binding	2.41732321506551	bayes_pls_golite062009
protein binding	1.75479345161448	bayes_pls_golite062009
ATP binding	1.44435058039859	bayes_pls_golite062009
adenyl ribonucleotide binding	1.41005152726625	bayes_pls_golite062009
adenyl nucleotide binding	1.38971642420542	bayes_pls_golite062009
ribonucleotide binding	1.16863558166547	bayes_pls_golite062009
purine ribonucleotide binding	1.16859376715941	bayes_pls_golite062009
purine nucleotide binding	1.15633908642115	bayes_pls_golite062009
nucleotide binding	1.14964488472581	bayes_pls_golite062009
AMP-activated protein kinase activity	1.12923456567012	bayes_pls_golite062009
catalytic activity	0.836142537655721	bayes_pls_golite062009
magnesium ion binding	0.439688030503848	bayes_pls_golite062009

Predicted Domain #5

Region A:
Residues: [774-865]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KKDDSYQTNS KNHYITASNM QTSHQVSKDL PAPTMVQNKC TLETKKAVRS NRSSIMVSEV  60
   61 NKASVDNKAA QSPEHSTAKR VLGFFKRRSM KI

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: