YRC Public Data Repository - Protein Structure Prediction

Protein:	GLN4
Organism:	Saccharomyces cerevisiae
Length:	809 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GLN4.

Description	E-value	Query Range	Subject Range
GLN4 - Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminyla... gi\|207341089 - gi\|207341089\|gb\|EDZ69240.1\| YOR168Wp-like protein [Saccharomyces cerevisiae AWRI1631] gi\|190407487 - gi\|190407487\|gb\|EDV10754.1\| glutamine-tRNA ligase [Saccharomyces cerevisiae RM11-1a] SYQ_YEAST - Glutamine--tRNA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GLN4 PE=1 SV=2	0.0	[1..809]	[1..809]
QARS - glutaminyl-tRNA synthetase gi\|168277804 - gi\|168277804\|dbj\|BAG10880.1\| glutaminyl-tRNA synthetase [synthetic construct] tr\|M0RDR7\|M0RDR7_RAT - Protein LOC100911132 OS=Rattus norvegicus GN=LOC100911132 PE=3 SV=1	0.0	[1..809]	[1..774]
SYQ_LUPLU - Glutamine--tRNA ligase OS=Lupinus luteus PE=2 SV=2	0.0	[2..809]	[9..791]
SYQ_ARATH - Glutamine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=OVA9 PE=2 SV=1	0.0	[5..809]	[9..792]
SPBC342.02 - glutaminyl-tRNA synthetase SYQ_SCHPO - Probable glutamine--tRNA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=qrs1 PE=3 ...	0.0	[3..809]	[4..808]
gi\|220943764 - gi\|220943764\|gb\|ACL84425.1\| Aats-gln-PA [synthetic construct] Aats-gln-PA - The gene Glutaminyl-tRNA synthetase is referred to in FlyBase by the symbol Dmel\Aats-gln (CG10506, ...	0.0	[5..809]	[4..777]
ers-1 - tRNA synthetases class I (E and Q) status:Partially_confirmed UniProt:O62431 protein_id:CAB08998.1	0.0	[1..806]	[1..780]
gi\|18606505 - gi\|18606505\|gb\|AAH23023.1\| Qars protein [Mus musculus]	0.0	[171..809]	[1..605]
SYQ_VIBCM - Glutamine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain M66-2) GN=glnS PE=3 SV=1 gi\|229357627, gi... - gi\|229505404\|ref\|ZP_04394914.1\| glutaminyl-tRNA synthetase [Vibrio cholerae BX 330286], gi\|229357627... SYQ_VIBCH - Glutamine--tRNA ligase OS=Vibrio cholerae GN=glnS PE=3 SV=1 gi\|229512441, gi... - gi\|229512441\|ref\|ZP_04401914.1\| glutaminyl-tRNA synthetase [Vibrio cholerae TMA 21], gi\|229350522\|gb... gi\|75823318, gi\|... - gi\|75823318\|ref\|ZP_00752828.1\| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Vibrio cholerae R... gi\|229518047, gi... - gi\|229518047\|ref\|ZP_04407491.1\| glutaminyl-tRNA synthetase [Vibrio cholerae RC9], gi\|229344762\|gb\|EE... gi\|254848127, gi... - gi\|75829808\|ref\|ZP_00759100.1\| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Vibrio cholerae M... gi\|229523212, gi... - gi\|229523212\|ref\|ZP_04412619.1\| glutaminyl-tRNA synthetase [Vibrio cholerae TM 11079-80], gi\|2293395... gi\|125621264, gi... - gi\|75818712\|ref\|ZP_00748842.1\| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Vibrio cholerae V... gi\|229529909, gi... - gi\|229529909\|ref\|ZP_04419299.1\| glutaminyl-tRNA synthetase [Vibrio cholerae 12129(1)], gi\|229333683\|... gi\|121629583, gi... - gi\|75816357\|ref\|ZP_00746830.1\| COG0008: Glutamyl- and glutaminyl-tRNA synthetases [Vibrio cholerae V... gi\|229371078, gi... - gi\|229608423\|ref\|YP_002879071.1\| glutaminyl-tRNA synthetase [Vibrio cholerae MJ-1236], gi\|229371078\|... gi\|254291870, gi... - gi\|254291870\|ref\|ZP_04962653.1\| glutaminyl-tRNA synthetase [Vibrio cholerae AM-19226], gi\|197750805\|... gi\|149740045, gi... - gi\|153826185\|ref\|ZP_01978852.1\| glutaminyl-tRNA synthetase [Vibrio cholerae MZO-2], gi\|149740045\|gb\|... gi\|121587357, gi... - gi\|121587357\|ref\|ZP_01677128.1\| glutaminyl-tRNA synthetase [Vibrio cholerae 2740-80], gi\|121548440\|g... gi\|153216044, gi... - gi\|153216044\|ref\|ZP_01950249.1\| glutaminyl-tRNA synthetase [Vibrio cholerae 1587], gi\|124114494\|gb\|E... gi\|153222787, gi... - gi\|153222787\|ref\|ZP_01953446.1\| glutaminyl-tRNA synthetase [Vibrio cholerae MAK 757], gi\|124118661\|g... gi\|126510845, gi... - gi\|153818619\|ref\|ZP_01971286.1\| glutaminyl-tRNA synthetase [Vibrio cholerae NCTC 8457], gi\|126510845... gi\|126518828, gi... - gi\|229510926\|ref\|ZP_04400405.1\| glutaminyl-tRNA synthetase [Vibrio cholerae B33], gi\|229350891\|gb\|EE... SYQ_VIBCH - Glutamine--tRNA ligase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN=g...	0.0	[232..809]	[7..552]

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Predicted Domain #1

Region A:
Residues: [1-198]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSVEELTQL FSQVGFEDKK VKEIVKNKKV SDSLYKLIKE TPSDYQWNKS TRALVHNLAS  60
   61 FVKGTDLPKS ELIVNGIING DLKTSLQVDA AFKYVKANGE ASTKMGMNEN SGVGIEITED 120
  121 QVRNYVMQYI QENKERILTE RYKLVPGIFA DVKNLKELKW ADPRSFKPII DQEVLKLLGP 180
  181 KDERDLIKKK TKNNEKKK

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [199-488]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TNSAKKSSDN SASSGPKRTM FNEGFLGDLH KVGENPQAYP ELMKEHLEVT GGKVRTRFPP  60
   61 EPNGYLHIGH SKAIMVNFGY AKYHNGTCYL RFDDTNPEKE APEYFESIKR MVSWLGFKPW 120
  121 KITYSSDYFD ELYRLAEVLI KNGKAYVCHC TAEEIKRGRG IKEDGTPGGE RYACKHRDQS 180
  181 IEQNLQEFRD MRDGKYKPGE AILRMKQDLN SPSPQMWDLI AYRVLNAPHP RTGTKWRIYP 240
  241 TYDFTHCLVD SMENITHSLC TTEFYLSRES YEWLCDQVHV FRPAQREYGR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1306.0
Match:	1qrtA_
Description:	Gln-tRNA synthetase (GlnRS), C-terminal (anticodon-binding) domain; Glutaminyl-tRNA synthetase (GlnRS)
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [489-559]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LNITGTVLSK RKIAQLVDEK FVRGWDDPRL FTLEAIRRRG VPPGAILSFI NTLGVTTSTT  60
   61 NIQVVRFESA V

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [707-729]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NQLFKSENPS SHPEGFLKDI NPE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1306.0
Match:	1qrtA_
Description:	Gln-tRNA synthetase (GlnRS), C-terminal (anticodon-binding) domain; Glutaminyl-tRNA synthetase (GlnRS)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
glutamine-tRNA ligase activity	7.66556935228325	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
lysine-tRNA ligase activity	4.64665624411373	bayes_pls_golite062009
structural constituent of ribosome	4.61484562096458	bayes_pls_golite062009
structural molecule activity	4.261982318682	bayes_pls_golite062009
glutamate-tRNA ligase activity	2.63681738794687	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
5S rRNA binding	2.25646775578129	bayes_pls_golite062009
rRNA binding	2.12571610259264	bayes_pls_golite062009
methionine-tRNA ligase activity	2.01261843164558	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
arginine-tRNA ligase activity	1.13743634264647	bayes_pls_golite062009
nucleotidyltransferase activity	1.06535308346284	bayes_pls_golite062009
cysteine-tRNA ligase activity	0.844943947059557	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009
binding	0.47302762432927	bayes_pls_golite062009
nucleic acid binding	0.161799496958314	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [560-706]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RKYLEDTTPR LMFVLDPVEV VVDNLSDDYE ELATIPYRPG TPEFGERTVP FTNKFYIERS  60
   61 DFSENVDDKE FFRLTPNQPV GLIKVSHTVS FKSLEKDEAG KIIRIHVNYD NKVEEGSKPK 120
  121 KPKTYIQWVP ISSKYNSPLR VTETRVY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [730-809]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SEVVYKESVM EHNFGDVVKN SPWVVDSVKN SEFYVEEDKD SKEVCRFQAM RVGYFTLDKE  60
   61 STTSKVILNR IVSLKDATSK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	1306.0
Match:	1qrtA_
Description:	Gln-tRNA synthetase (GlnRS), C-terminal (anticodon-binding) domain; Glutaminyl-tRNA synthetase (GlnRS)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: