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View Structure Prediction Details

Protein: PRK1
Organism: Saccharomyces cerevisiae
Length: 810 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PRK1.

Description E-value Query
Range
Subject
Range
PRK1 - Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton t...
PRK1_YEAST - Actin-regulating kinase PRK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRK1 PE=1 S...
gi|151943072 - gi|151943072|gb|EDN61407.1| serine/threonine protein kinase [Saccharomyces cerevisiae YJM789]
0.0 [1..810] [1..810]
gi|9845487, gi|1... - ref|NP_059672.1| MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens], gi|1749794|...
0.0 [20..718] [18..739]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
0.0 [20..718] [18..739]
MRK2_MOUSE - (Q05512) MAP/microtubule affinity-regulating kinase 2 (EC 2.7.1.37) (Serine/threonine-protein kinase...
0.0 [20..673] [51..716]
MARK3_RAT - MAP/microtubule affinity-regulating kinase 3 OS=Rattus norvegicus GN=Mark3 PE=1 SV=1
0.0 [20..673] [54..739]

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Predicted Domain #1
Region A:
Residues: [1-70]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNTPQISLYE PGTILTVGSH HAKIIKYLTS GGFAQVYTAE ISPPDPYSNA NIACLKRVIV  60
   61 PHKQGLNTLR 

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [89-109]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IDSHAARSVN GIAYEVFVLM E

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 222.201249
Match: 2phkA_
Description: gamma-subunit of glycogen phosphorylase kinase (Phk)
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [71-88]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AEVDAMKLLR NNKHVVSY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [110-351]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FCERGGLIDF MNTRLQNRLQ ESEILEIMSQ TVQGITAMHA LQPPLIHRDI KIENVLISHD  60
   61 GLYKVCDFGS VSGVIRPPRN TQEFNYVQHD ILTNTTAQYR SPEMIDLYRG LPIDEKSDIW 120
  121 ALGVFLYKIC YYTTPFEKSG EAGILHARYQ YPSFPQYSDR LKNLIRLMLM EAPSQRPNIC 180
  181 QVLEEVSRLQ NKPCPIRNFY LLRAMNQNAN TQLAGEPSST TYVPTQKFIP VQSLQSINQP 240
  241 PN

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 222.201249
Match: 2phkA_
Description: gamma-subunit of glycogen phosphorylase kinase (Phk)
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [352-810]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMPVTHVSTT PNLGTFPISI NDNNKTEVTA HAGLQVGSHS NLTSPLMKTK SVPLSDEFAS  60
   61 LYYKELHPFQ KSQTFKSVES FQSPQRKSMP PLSLTPVNND IFDRVSAINR PNNYVDSETQ 120
  121 TIDNMAVPNL KLSPTITSKS LSSTKEIAAP DNINGSKIVR SLSSKLKKVI TGESRGNSPI 180
  181 KSRQNTGDSI RSAFGKLRHG FTGNSVNNSR SASFDNNNVN GNGNNTNRRL VSSSTSSFPK 240
  241 FNSDTKRKEE SDKNQRLEKR RSMPPSILSD FDQHERNNSR TGSRDYYRSH SPVKKTQASA 300
  301 KTTSKPTLIP DNGNVNINQE KKESIQRRVH NLLKSSDDPV TYKSASGYGK YTDIGTETSN 360
  361 RHSSVRITPI TEEKFKKTLK DGVLDIKTKS NGKDKSRPPR PPPKPLHLRT EIQKIRNFSR 420
  421 LQSKKLPIER ISSEATETIV DVNVDDLEAD FRKRFPSKV

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term Confidence Notes
protein kinase activity 4.05475891009228 bayes_pls_golite062009
protein serine/threonine kinase activity 3.7974128326421 bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor 3.59230737331367 bayes_pls_golite062009
kinase activity 3.41737435609334 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 3.2342722637405 bayes_pls_golite062009
binding 2.41732321506551 bayes_pls_golite062009
transferase activity 2.04713540681686 bayes_pls_golite062009
protein binding 1.75479345161448 bayes_pls_golite062009
ATP binding 1.44435058039859 bayes_pls_golite062009
adenyl ribonucleotide binding 1.41005152726625 bayes_pls_golite062009
adenyl nucleotide binding 1.38971642420542 bayes_pls_golite062009
ribonucleotide binding 1.16863558166547 bayes_pls_golite062009
purine ribonucleotide binding 1.16859376715941 bayes_pls_golite062009
purine nucleotide binding 1.15633908642115 bayes_pls_golite062009
nucleotide binding 1.14964488472581 bayes_pls_golite062009
catalytic activity 0.836142537655721 bayes_pls_golite062009
AMP-activated protein kinase activity 0.496765880715405 bayes_pls_golite062009
magnesium ion binding 0.439688030503848 bayes_pls_golite062009

Predicted Domain #4
Region A:
Residues: [709-810]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TSNRHSSVRI TPITEEKFKK TLKDGVLDIK TKSNGKDKSR PPRPPPKPLH LRTEIQKIRN  60
   61 FSRLQSKKLP IERISSEATE TIVDVNVDDL EADFRKRFPS KV

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle