Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #05 Alpha Factor Prep2-TiO2 Flowthrough |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 132 | 291 | 65.5% | 0.0635 | 195140 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 5 | 98 | 16.0% | 0.0943 | 42826 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 23 | 42 | 32.0% | 0.0163 | 111782 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 10 | 28 | 17.3% | 0.0122 | 93289 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 10 | 14 | 24.9% | 0.00911 | 61495 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 6 | 13 | 19.7% | 0.0109 | 46914 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 9 | 13 | 15.9% | 0.00519 | 99619 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 6 | 12 | 21.8% | 0.0121 | 42271 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 7 | 10 | 79.7% | 0.0531 | 7729 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 6 | 9 | 10.5% | 0.00568 | 67400 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 2 | 8 | 9.5% | 0.0116 | 29280 |
[ |
HEF3 | Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... | 2 | 8 | 2.3% | 0.00281 | 115869 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 5 | 7 | 5.4% | 0.00447 | 66087 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 5 | 7 | 4.2% | 0.00137 | 218454 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 2 | 5 | 0.9% | 9.77E-4 | 214849 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 3 | 4 | 12.4% | 0.00335 | 46816 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 3 | 3 | 15.0% | 0.00345 | 37354 |
[ |
YTM1 | Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... | 2 | 3 | 10.7% | 0.00239 | 51359 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 3 | 3 | 5.6% | 0.00185 | 66953 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 3 | 3 | 5.4% | 0.00156 | 80900 |
[ |
TCP1 | Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... | 2 | 3 | 2.9% | 0.00197 | 60481 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 2 | 2 | 1.5% | 5.48E-4 | 151652 |