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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 95 110 95.6% 0.123 42271
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 65 69 95.3% 0.111 29280
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 29 30 92.8% 0.177 7729
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 300 358 89.8% 0.0869 195140
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 12 15 85.0% 0.0416 16135
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 164 193 83.5% 0.0833 111782
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 19 20 81.4% 0.0506 18751
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 82 97 73.5% 0.068 67400
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 35 38 63.8% 0.027 66087
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 16 16 55.8% 0.0204 37354
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 58 61 41.2% 0.0292 94104
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 9 9 29.2% 0.00947 44585
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 12 12 27.5% 0.0127 45384
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 6 6 22.3% 0.00358 74468
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 3 3 21.2% 0.00448 29995
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 7 7 19.0% 0.00458 72105
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 4 4 13.7% 0.00178 99619
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 4 4 13.2% 0.00376 50653
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 16 16 12.9% 0.00348 218454
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 2 2 12.3% 0.00172 52628
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 3 10.7% 0.00145 93289
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 4 4 9.0% 0.00193 98227
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 4 4 8.3% 0.00122 151652
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 7 7 7.9% 0.00187 175459
[View Peptides]   YTM1 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... 2 2 7.8% 0.00177 51359
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 4 4 7.7% 0.00231 80900
[View Peptides]   HEF3 Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... 3 3 6.1% 0.00117 115869
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 3 3 5.8% 0.00148 96825
[View Peptides]   SPB1 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... 3 3 3.2% 0.00145 96485
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 2 2 2.1% 4.34E-4 214849

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